##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1331908
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77649357162807	31.0	31.0	34.0	30.0	34.0
2	31.943821194857303	31.0	31.0	34.0	30.0	34.0
3	32.07008667265307	33.0	31.0	34.0	30.0	34.0
4	35.72862915456623	37.0	35.0	37.0	35.0	37.0
5	35.634734531213866	37.0	35.0	37.0	33.0	37.0
6	35.67924661463104	37.0	35.0	37.0	33.0	37.0
7	35.53919039453176	37.0	35.0	37.0	33.0	37.0
8	35.57081194797238	37.0	35.0	37.0	33.0	37.0
9	37.19011523318427	39.0	37.0	39.0	33.0	39.0
10	37.05318685674987	39.0	37.0	39.0	33.0	39.0
11	37.115667898984015	39.0	37.0	39.0	33.0	39.0
12	36.97027422314454	39.0	37.0	39.0	33.0	39.0
13	37.02229733585202	39.0	37.0	39.0	33.0	39.0
14	38.069847166621116	40.0	37.0	41.0	33.0	41.0
15	38.07621697594729	40.0	37.0	41.0	33.0	41.0
16	37.984408082239916	40.0	37.0	41.0	33.0	41.0
17	38.006173099042876	40.0	37.0	41.0	33.0	41.0
18	37.97754424479769	40.0	37.0	41.0	33.0	41.0
19	38.037593437384565	40.0	37.0	41.0	33.0	41.0
20	37.935265048336674	40.0	37.0	41.0	32.0	41.0
21	37.80239776320887	40.0	37.0	41.0	32.0	41.0
22	37.689887739994056	40.0	37.0	41.0	32.0	41.0
23	37.508021575063744	39.0	36.0	41.0	32.0	41.0
24	37.65439504830664	40.0	37.0	41.0	32.0	41.0
25	37.620018800097306	40.0	37.0	41.0	32.0	41.0
26	37.418678317121	39.0	36.0	41.0	32.0	41.0
27	37.372618078726155	39.0	36.0	41.0	32.0	41.0
28	37.223881829675925	39.0	36.0	41.0	31.0	41.0
29	37.009356502100744	39.0	36.0	41.0	31.0	41.0
30	36.86041453313592	39.0	35.0	41.0	30.0	41.0
31	36.642962576994805	39.0	35.0	40.0	30.0	41.0
32	36.48377590644399	39.0	35.0	40.0	30.0	41.0
33	36.33019022334876	38.0	35.0	40.0	30.0	41.0
34	36.134372644356816	38.0	35.0	40.0	30.0	41.0
35	35.95436096186824	38.0	35.0	40.0	29.0	41.0
36	35.897742937199865	38.0	35.0	40.0	28.0	41.0
37	35.763803505947855	38.0	35.0	40.0	27.0	41.0
38	35.63131387453188	38.0	35.0	40.0	27.0	41.0
39	35.49006387828589	38.0	35.0	40.0	26.0	41.0
40	35.3243474774534	38.0	34.0	40.0	25.0	41.0
41	35.20769302384249	38.0	34.0	40.0	24.0	41.0
42	34.94383621090946	38.0	34.0	40.0	23.0	41.0
43	34.57433396300645	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	5.0
16	11.0
17	29.0
18	95.0
19	214.0
20	423.0
21	828.0
22	1622.0
23	2651.0
24	4340.0
25	6756.0
26	9966.0
27	14263.0
28	19146.0
29	25742.0
30	32507.0
31	40125.0
32	49579.0
33	61912.0
34	77658.0
35	96919.0
36	127746.0
37	186042.0
38	252234.0
39	321087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2039720461173	21.184270985683696	14.632767428380939	22.978989539818066
2	18.15936235836109	23.633238932418756	35.90142862720248	22.305970082017677
3	18.843568775020497	24.740672779200963	33.247942050051506	23.16781639572703
4	13.389288149031314	16.555197506134057	36.594269273853754	33.46124507098088
5	12.330356150725125	38.80425675046625	34.96172408304478	13.90366301576385
6	31.56929757911207	38.42262378482598	14.091063346717641	15.917015289344308
7	25.286731515990596	33.981175877012525	21.71824180048472	19.01385080651216
8	26.894950702300758	34.805256819540084	19.359069845665015	18.940722632494136
9	24.675728353610012	14.72173753742751	22.026070869759774	38.57646323920271
10	16.183550215180027	28.426963423900148	33.12781363277343	22.26167272814639
11	33.2792505188046	22.999936932580926	23.503800562801636	20.21701198581283
12	21.122555011307085	27.864462109995586	30.97969229105914	20.033290587638184
13	31.523348459503207	21.505464341380936	26.072521525510773	20.898665673605084
14	20.374530372968703	22.96134567853035	28.623298305888994	28.04082564261195
15	22.490817684104307	30.280019340675178	25.416094805346916	21.813068169873596
16	21.501710328340998	28.652429447078926	26.60731822318058	23.238542001399495
17	21.39194298705316	27.44979382960385	27.63456635142968	23.523696831913316
18	22.719437078236634	26.001270358012718	29.86467533793625	21.414617225814396
19	22.663352123419937	25.434414388981825	31.12234478657685	20.77988870102139
20	23.248527676085736	25.18537316391222	30.61607858801058	20.95002057199146
21	22.09094021508993	25.858317541451814	31.156806626283494	20.893935617174762
22	21.184946708030886	26.289278238436893	30.753325304750778	21.772449748781447
23	21.93041861750211	26.246857891085572	31.1752012901792	20.64752220123312
24	21.71201013883842	26.375094976529912	29.837045801962297	22.075849082669375
25	21.620111899620696	26.47893097721464	30.68162365568793	21.219333467476734
26	21.158893857533702	27.362775807338046	30.61022232766828	20.868108007459973
27	20.399757340597098	27.23558984554489	30.368313727374563	21.996339086483452
28	20.818104553767977	27.04060640825042	30.35607564486436	21.785213393117242
29	21.02547623409425	26.947807205903114	29.697846998441335	22.32886956156131
30	21.08824333212204	27.196247788886318	31.121819224751256	20.593689654240382
31	21.750225991585005	26.904035413857414	30.835688350847057	20.510050243710527
32	21.016766923841587	26.929487622268205	30.163419695654653	21.890325758235555
33	21.0097844595873	26.505584469798215	30.799349504620437	21.685281565994046
34	20.248770936130725	26.751397243653464	31.48107827267349	21.51875354754232
35	19.901449649675502	28.300603344975777	30.60519195019476	21.19275505515396
36	21.697895049808245	26.497625962153542	30.510365580805882	21.29411340723233
37	20.80091117404505	26.95035993477027	30.55699042276193	21.691738468422745
38	20.394351561819583	25.820176768966025	31.827573676259924	21.957897992954468
39	20.022779351126356	25.506416359087865	32.44585962393799	22.024944665847794
40	19.06392934046496	25.805461037849458	32.61156175952093	22.519047862164655
41	19.86803893362004	25.0871681827874	32.68243752571499	22.362355357877572
42	19.035549001883012	26.61415052691327	32.007390900873034	22.342909570330683
43	19.05829832090505	26.467443697312426	31.6735840613616	22.80067392042093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	763.0
1	661.0
2	559.0
3	1111.5
4	1664.0
5	1664.0
6	2317.5
7	2971.0
8	3089.0
9	3207.0
10	4853.0
11	6499.0
12	6499.0
13	11062.5
14	15626.0
15	26749.0
16	37872.0
17	34945.0
18	32018.0
19	32018.0
20	32636.0
21	33254.0
22	27174.0
23	21094.0
24	23516.0
25	25938.0
26	25938.0
27	28843.0
28	31748.0
29	34053.0
30	36358.0
31	39409.5
32	42461.0
33	42461.0
34	47027.5
35	51594.0
36	56058.0
37	60522.0
38	63850.5
39	67179.0
40	67179.0
41	69725.0
42	72271.0
43	75584.5
44	78898.0
45	83797.5
46	88697.0
47	88697.0
48	110306.0
49	131915.0
50	121952.0
51	111989.0
52	101927.0
53	91865.0
54	91865.0
55	83207.5
56	74550.0
57	65311.5
58	56073.0
59	50601.0
60	45129.0
61	45129.0
62	39696.5
63	34264.0
64	29400.5
65	24537.0
66	20848.5
67	17160.0
68	17160.0
69	14685.5
70	12211.0
71	10342.0
72	8473.0
73	6912.5
74	5352.0
75	5352.0
76	4330.0
77	3308.0
78	2556.5
79	1805.0
80	1425.5
81	1046.0
82	1046.0
83	781.5
84	517.0
85	386.0
86	255.0
87	194.0
88	133.0
89	133.0
90	106.0
91	79.0
92	54.0
93	29.0
94	20.0
95	11.0
96	11.0
97	11.0
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1331908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.23342011104431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9326911480214	47.46521553414195
2	9.950137696670748	11.389608219125913
3	2.8454086807219197	4.885574112341098
4	1.2780384330927403	2.9258604223703037
5	0.7056275376718393	2.0192738652747058
6	0.45266872529309476	1.5544667595498358
7	0.311231156958083	1.2468976490479835
8	0.22518781782227176	1.0310615185049112
9	0.16490658840832179	0.8494351248107296
>10	1.0073163367716482	10.838719715187706
>50	0.07798600721601355	3.0844118796142266
>100	0.04169694733127744	4.388819320078972
>500	0.003814533270350865	1.533952954145588
>1k	0.002762248230254074	2.7042169251012793
>5k	1.3153563001209879E-4	0.4294857481056429
>10k+	3.9460689003629633E-4	3.6530002525991616
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19742	1.4822345086897895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14432	1.0835583238481936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14350	1.0774017424626925	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5705	0.42833288785711926	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3419	0.2566994116710764	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2770	0.20797232241265914	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2665	0.20008889502878577	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2577	0.1934818320784919	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2575	0.19333167155689432	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2163	0.16239860410779122	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2137	0.1604465173270226	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.1343185865690423	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1690	0.1268856407499617	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1430	0.1073647729422753	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1411	0.10593824798709821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.508026079879392E-5	0.0
10	0.0	0.0	0.0	1.5016052159758783E-4	0.0
11	0.0	0.0	0.0	6.757223471891452E-4	0.0
12	0.0	0.0	0.0	8.25882868786733E-4	0.0
13	0.0	7.508026079879392E-5	0.0	8.25882868786733E-4	0.0
14	0.0	7.508026079879392E-5	0.0	9.00963129585527E-4	0.0
15	0.0	7.508026079879392E-5	0.0	0.0010511236511831148	0.0
16	0.0	7.508026079879392E-5	0.0	0.0012763644335794964	0.0
17	0.0	7.508026079879392E-5	0.0	0.0021022473023662295	0.0
18	0.0	7.508026079879392E-5	0.0	0.0024025683455614053	0.0
19	0.0	7.508026079879392E-5	0.0	0.0030782906927505505	0.0
20	0.0	7.508026079879392E-5	0.0	0.006607062950293864	0.0
21	0.0	7.508026079879392E-5	0.0	0.012238082510203408	0.0
22	0.0	7.508026079879392E-5	0.0	0.01959594806848521	0.0
23	0.0	7.508026079879392E-5	0.0	0.02455124528120561	0.0
24	0.0	1.5016052159758783E-4	0.0	0.03235959240428018	0.0
25	0.0	1.5016052159758783E-4	0.0	0.0370145685738054	0.0
26	0.0	1.5016052159758783E-4	0.0	0.04429735387128841	0.0
27	0.0	1.5016052159758783E-4	0.0	0.10683921111668374	0.0
28	0.0	1.5016052159758783E-4	0.0	0.26593428374932804	0.0
29	0.0	1.5016052159758783E-4	0.0	0.4874961333665689	0.0
30	0.0	1.5016052159758783E-4	0.0	0.792397072470471	0.0
31	7.508026079879392E-5	1.5016052159758783E-4	0.0	1.2402508281352767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	375	0.0	23.186665	1
GGTATCA	4320	0.0	22.996529	1
ATTGGAC	395	0.0	21.075949	3
TCTATAC	125	1.8189894E-11	20.72	3
ACGTTCG	155	0.0	20.290321	22
CGCACTA	65	6.903184E-5	19.923077	29
CGTTCGC	180	0.0	19.527779	23
TAAACGT	95	1.6766171E-7	19.473684	4
TTGGACC	565	0.0	19.318584	4
CGTCTTA	50	0.0070354533	18.5	33
GACCCTC	575	0.0	18.33913	7
GGACCCT	595	0.0	18.033613	6
ACCCTCG	570	0.0	17.850876	8
TAGGTCG	65	0.0015801124	17.076923	21
TGGACCC	670	0.0	16.843285	5
TATTGGA	555	0.0	16.666666	2
TTCGTTA	185	1.8189894E-11	16.000002	30
CGCGCAA	70	0.0025927837	15.857142	9
ATTATAC	225	0.0	15.622222	3
CAGTTCG	215	0.0	15.488372	13
>>END_MODULE