FastQCFastQC Report
Wed 25 May 2016
SRR2088532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425616
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT244211.7130138831214015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178121.2494248100470253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT175841.2334317235496797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66980.4698319884176384No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG48150.33774873458210347No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38260.2683752146440556No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA38090.26718274766837635No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38010.2666215867386449No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA36680.2572922862818599No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30510.21401274957632352No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28220.19794951796276136No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC24640.17283756635727995No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.14870764637882852No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT21120.1481464854490971No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT19010.13334586592743067No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17850.12520903244632497No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17690.1240867105868621No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG16730.11735277943008496No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG15870.1113202994354721No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA15820.11096957385438996No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT15260.10704144734626996No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15190.10655043153275495No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT14600.10241186967598567No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14350.10065824177057496No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14330.10051795153814211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.025.089041
GTATTGG4300.022.8023241
GGACCCT6200.019.395166
TGGACCC6350.019.2283465
ATTGGAC5150.018.6796113
GTTCTAC902.152954E-618.51
AACCCGC609.238299E-418.56
CCCGCGT609.238299E-418.58
TTGGACC6900.018.2318844
CTAGGCA1750.017.971434
GACCCTC6400.017.343757
GACCGTG650.001580186117.0769237
TATTGGA6200.016.7096772
CGAACTA1005.8797814E-616.65000224
GTATTAG2100.015.8571421
ACCCTCG7250.015.5655178
ACTCTGT6900.015.55072437
ACTATGA1557.212293E-915.5161298
ACGTTCG855.3652754E-415.23529322
AAGACCG855.3652754E-415.2352935
Produced by FastQC (version 0.11.5)