##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088532_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1425616
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812420034567513	31.0	31.0	34.0	30.0	34.0
2	31.97386673550241	31.0	31.0	34.0	30.0	34.0
3	32.087654740126375	33.0	31.0	34.0	30.0	34.0
4	35.74080678106868	37.0	35.0	37.0	35.0	37.0
5	35.662496773324655	37.0	35.0	37.0	33.0	37.0
6	35.7179584123635	37.0	35.0	37.0	33.0	37.0
7	35.57555190177439	37.0	35.0	37.0	33.0	37.0
8	35.6180570363969	37.0	35.0	37.0	33.0	37.0
9	37.23167318548613	39.0	37.0	39.0	34.0	39.0
10	37.10232769553652	39.0	37.0	39.0	33.0	39.0
11	37.16162557098124	39.0	37.0	39.0	33.0	39.0
12	37.01679554662686	39.0	37.0	39.0	33.0	39.0
13	37.066130711215365	39.0	37.0	39.0	33.0	39.0
14	38.1023522463272	40.0	37.0	41.0	33.0	41.0
15	38.10836227988462	40.0	37.0	41.0	33.0	41.0
16	38.01645043265508	40.0	37.0	41.0	33.0	41.0
17	38.05995653808599	40.0	37.0	41.0	33.0	41.0
18	38.035066946498915	40.0	37.0	41.0	33.0	41.0
19	38.10985566940887	40.0	37.0	41.0	33.0	41.0
20	38.015739862627804	40.0	37.0	41.0	33.0	41.0
21	37.8874121783145	40.0	37.0	41.0	32.0	41.0
22	37.7801469680475	40.0	37.0	41.0	32.0	41.0
23	37.61141850259818	40.0	36.0	41.0	32.0	41.0
24	37.74955527996319	40.0	37.0	41.0	32.0	41.0
25	37.71180668567132	40.0	37.0	41.0	32.0	41.0
26	37.5154915489164	40.0	37.0	41.0	32.0	41.0
27	37.47452469669252	40.0	36.0	41.0	32.0	41.0
28	37.32133056868049	39.0	36.0	41.0	32.0	41.0
29	37.10662338245362	39.0	36.0	41.0	31.0	41.0
30	36.94285557962312	39.0	35.0	41.0	31.0	41.0
31	36.68976638870495	39.0	35.0	41.0	30.0	41.0
32	36.54144734626996	39.0	35.0	40.0	30.0	41.0
33	36.376770462733305	38.0	35.0	40.0	30.0	41.0
34	36.184916555369746	38.0	35.0	40.0	30.0	41.0
35	35.98938213375832	38.0	35.0	40.0	29.0	41.0
36	35.90814216450994	38.0	35.0	40.0	29.0	41.0
37	35.764792903558885	38.0	35.0	40.0	27.0	41.0
38	35.62912733863818	38.0	35.0	40.0	27.0	41.0
39	35.480442840147695	38.0	35.0	40.0	26.0	41.0
40	35.296462020628276	38.0	35.0	40.0	25.0	41.0
41	35.15169933558546	38.0	34.0	40.0	24.0	41.0
42	34.86115686131469	38.0	34.0	40.0	23.0	41.0
43	34.46931221310648	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	4.0
16	10.0
17	52.0
18	68.0
19	161.0
20	423.0
21	817.0
22	1607.0
23	2683.0
24	4511.0
25	7135.0
26	10371.0
27	14708.0
28	20493.0
29	27073.0
30	34229.0
31	42164.0
32	52299.0
33	65551.0
34	81522.0
35	102077.0
36	136884.0
37	202750.0
38	267223.0
39	350797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86317774211288	21.63955791741956	15.159481936229671	22.33778240423789
2	18.17852773818476	24.117293857532463	36.20771652394474	21.496461880338043
3	18.70973670329177	24.373183241489997	34.39909484742034	22.517985207797892
4	12.982598399569028	16.481226361095835	36.89829519309548	33.63788004623966
5	11.69922335327325	39.29227786444597	35.24707915736075	13.761419624920034
6	31.164913974029474	38.72592619611452	13.71828037844693	16.390879451409074
7	24.562504910158133	34.896564011627255	21.304544842369893	19.236386235844716
8	26.988403609387102	34.887234711170464	19.133272915006565	18.991088764435865
9	24.30352914108708	14.719812347785096	22.624184913749566	38.35247359737826
10	15.988106194094343	28.78713482452498	33.13472912761922	22.09002985376146
11	32.78077687119112	23.109729408199684	23.955819800002246	20.153673920606952
12	21.186981627591162	28.372366752337236	31.03135767275339	19.40929394731821
13	31.74880192141502	21.910458356247407	26.320060942076967	20.020678780260603
14	19.909288335708915	23.728339188112365	29.12789979910439	27.234472677074333
15	21.66936959181154	31.07561924108596	25.92528422801091	21.329726939091593
16	20.782244307022367	29.284744278964318	26.961467884759994	22.971543529253317
17	20.87729093949563	27.972399299671157	27.58842493350243	23.561884827330783
18	22.36149145351904	26.155430354317012	30.69985185351455	20.783226338649396
19	22.093887835153364	25.587465348312588	32.11993973131615	20.198707085217897
20	22.905957845590958	25.244245294665603	31.423468872403227	20.42632798734021
21	21.664669869025037	26.013667074443607	32.28218538512475	20.039477671406605
22	20.343556750204826	26.4939506851775	31.76984545627995	21.39264710833773
23	21.60020650722214	26.49900113354508	32.14435023175947	19.756442127473317
24	20.970022783133746	26.631645548310345	30.487382296494992	21.91094937206092
25	21.03280266214745	26.785614078405406	31.693036554022964	20.48854670542418
26	20.35990056228325	27.94721720294946	31.51311433092782	20.179767903839462
27	19.382708948272185	27.75985959753538	31.186308234475487	21.67112321971695
28	20.09201636345271	27.432983355966822	31.06453631272376	21.410463967856703
29	20.26366146283431	27.192736332925556	30.38013041380007	22.163471790440063
30	20.493807589140413	27.65892077529994	32.132285271770236	19.714986363789407
31	21.408850630183725	27.430387986666815	31.642742505695782	19.518018877453677
32	20.575877375113635	27.375183780204488	30.82821741619062	21.220721428491263
33	20.12582630946903	26.97956532474383	31.68447884984456	21.210129515942583
34	19.338798105520702	27.44069931875063	32.41714458872516	20.803357987003512
35	18.932166866814065	29.48199234576492	31.197812033534976	20.38802875388604
36	21.27375113635088	27.2193914770878	30.92235216215306	20.584505224408257
37	20.312412318604732	28.05159313587951	30.969559825366716	20.666434720149045
38	19.909639061289997	26.490794154947757	32.395329457581845	21.204237326180404
39	19.340341298077462	26.288916510476874	33.20326090616267	21.167481285282992
40	18.223771338144353	26.36362105924737	33.20178785872213	22.21081974388615
41	19.300849597647613	25.638881718499228	33.436002401768775	21.624266282084374
42	18.593436100604933	27.312544191423214	32.57412935881752	21.51989034915433
43	18.40614864030707	27.231105711495946	32.132215126654025	22.230530521542967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	552.0
1	537.0
2	522.0
3	1065.5
4	1609.0
5	1609.0
6	2377.0
7	3145.0
8	3410.5
9	3676.0
10	5364.5
11	7053.0
12	7053.0
13	12714.5
14	18376.0
15	31910.0
16	45444.0
17	42227.0
18	39010.0
19	39010.0
20	39817.5
21	40625.0
22	33172.5
23	25720.0
24	28640.5
25	31561.0
26	31561.0
27	34448.5
28	37336.0
29	39311.0
30	41286.0
31	44754.5
32	48223.0
33	48223.0
34	53317.5
35	58412.0
36	62815.5
37	67219.0
38	70101.0
39	72983.0
40	72983.0
41	74139.0
42	75295.0
43	78528.0
44	81761.0
45	87216.5
46	92672.0
47	92672.0
48	121250.0
49	149828.0
50	136687.5
51	123547.0
52	109858.5
53	96170.0
54	96170.0
55	84699.5
56	73229.0
57	62502.5
58	51776.0
59	46366.0
60	40956.0
61	40956.0
62	35819.0
63	30682.0
64	26571.0
65	22460.0
66	19102.0
67	15744.0
68	15744.0
69	13356.0
70	10968.0
71	9155.0
72	7342.0
73	5974.5
74	4607.0
75	4607.0
76	3628.5
77	2650.0
78	2070.0
79	1490.0
80	1169.0
81	848.0
82	848.0
83	639.5
84	431.0
85	320.0
86	209.0
87	152.5
88	96.0
89	96.0
90	79.0
91	62.0
92	42.0
93	22.0
94	18.5
95	15.0
96	15.0
97	8.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1425616.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.30796845280938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.85757051729227	45.826838962468294
2	10.202826201352428	11.285951793477938
3	2.9110981734982655	4.830207778286192
4	1.2304761877179466	2.7222055268894936
5	0.6478905677136045	1.7916755539988398
6	0.4225116691774346	1.4020957241885632
7	0.2686463790420367	1.0400799819912905
8	0.2094977919525496	0.926951781459587
9	0.15663391176933641	0.7796793105700726
>10	0.9486384135724194	10.055932420244554
>50	0.08499399119781205	3.2285283647035574
>100	0.04932574138781428	4.950485448486776
>500	0.0054524299490418685	2.1281218331463307
>1k	0.003930821591169719	4.36648942413435
>5k	1.268006964893458E-4	0.469736695943784
>10k+	3.804020894680373E-4	4.1950194000103505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24421	1.7130138831214015	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17812	1.2494248100470253	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17584	1.2334317235496797	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6698	0.4698319884176384	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4815	0.33774873458210347	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3826	0.2683752146440556	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3809	0.26718274766837635	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3801	0.2666215867386449	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3668	0.2572922862818599	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3051	0.21401274957632352	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2822	0.19794951796276136	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2464	0.17283756635727995	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2120	0.14870764637882852	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2112	0.1481464854490971	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1901	0.13334586592743067	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1785	0.12520903244632497	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1769	0.1240867105868621	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1673	0.11735277943008496	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1587	0.1113202994354721	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1582	0.11096957385438996	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1526	0.10704144734626996	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1519	0.10655043153275495	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1460	0.10241186967598567	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1435	0.10065824177057496	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1433	0.10051795153814211	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.014511621642854E-5	0.0	0.0	0.0	0.0
9	7.014511621642854E-5	0.0	0.0	0.0	0.0
10	7.014511621642854E-5	0.0	0.0	0.0	0.0
11	7.014511621642854E-5	0.0	0.0	2.805804648657142E-4	0.0
12	7.014511621642854E-5	0.0	0.0	7.71596278380714E-4	0.0
13	7.014511621642854E-5	0.0	0.0	7.71596278380714E-4	0.0
14	7.014511621642854E-5	0.0	0.0	0.0011223218594628567	0.0
15	7.014511621642854E-5	0.0	0.0	0.0014730474405449995	0.0
16	7.014511621642854E-5	0.0	0.0	0.0018939181378435708	0.0
17	7.014511621642854E-5	0.0	0.0	0.002314788835142142	0.0
18	7.014511621642854E-5	0.0	0.0	0.0028759497648735703	0.0
19	7.014511621642854E-5	0.0	0.0	0.003577400927037856	0.0
20	7.014511621642854E-5	0.0	0.0	0.006242915343262141	0.0
21	7.014511621642854E-5	0.0	0.0	0.011012783245979282	0.0
22	7.014511621642854E-5	0.0	0.0	0.01936005207573428	0.0
23	7.014511621642854E-5	0.0	0.0	0.023779194397369276	0.0
24	7.014511621642854E-5	0.0	0.0	0.02953109392711642	0.0
25	7.014511621642854E-5	0.0	0.0	0.032898059505504985	0.0
26	7.014511621642854E-5	0.0	0.0	0.04145576368390927	0.0
27	7.014511621642854E-5	0.0	0.0	0.10584898037059068	0.0
28	7.014511621642854E-5	0.0	0.0	0.25834446302510633	0.0
29	7.014511621642854E-5	0.0	0.0	0.4739705502744077	0.0
30	7.014511621642854E-5	0.0	0.0	0.7680890225698926	0.0
31	7.014511621642854E-5	0.0	0.0	1.1536767264116004	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5110	0.0	25.08904	1
GTATTGG	430	0.0	22.802324	1
GGACCCT	620	0.0	19.39516	6
TGGACCC	635	0.0	19.228346	5
ATTGGAC	515	0.0	18.679611	3
GTTCTAC	90	2.152954E-6	18.5	1
AACCCGC	60	9.238299E-4	18.5	6
CCCGCGT	60	9.238299E-4	18.5	8
TTGGACC	690	0.0	18.231884	4
CTAGGCA	175	0.0	17.97143	4
GACCCTC	640	0.0	17.34375	7
GACCGTG	65	0.0015801861	17.076923	7
TATTGGA	620	0.0	16.709677	2
CGAACTA	100	5.8797814E-6	16.650002	24
GTATTAG	210	0.0	15.857142	1
ACCCTCG	725	0.0	15.565517	8
ACTCTGT	690	0.0	15.550724	37
ACTATGA	155	7.212293E-9	15.516129	8
ACGTTCG	85	5.3652754E-4	15.235293	22
AAGACCG	85	5.3652754E-4	15.235293	5
>>END_MODULE