FastQCFastQC Report
Wed 25 May 2016
SRR2088531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840900
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164711.958734689023665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117191.3936258770365084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT113681.3518848852420027No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.5382328457604948No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32310.38423118087763114No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26610.31644666428826257No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26600.31632774408371983No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25560.30396004281127365No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23610.28077060292543704No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21200.25211083363063386No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG19190.22820787251754074No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16140.19193721013200143No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.18099655131406828No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12740.1515043405874658No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT12410.147579973837555No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT12360.14698537281484123No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12200.14508264954215722No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG10540.12534189558806041No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG10240.12177428945177786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9890.11761208229278154No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9770.11618503983826853No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9660.11487691758829824No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT9330.11095255083838744No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT9260.11012010940658819No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.10655250327030563No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA8860.1053633012248781No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC8780.1044119395885361No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA8670.10310381733856583No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8540.10155785467951006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT250.00549551629.5999986
ATTGGAC2950.024.4576263
GGTATCA42900.022.769231
TATTGGA3300.022.4242422
GTATTGG3450.021.9855081
CGAACGT656.899316E-519.92307712
GGACCCT4200.019.8214286
TTGGACC4700.018.54
CGCCGGA902.1508185E-618.514
GTGTATG752.0664357E-417.2666661
ACTATAG650.001579457517.0769233
CCGCTAT650.001579457517.07692325
GACCCTC4900.016.9897987
CTCTAAT1107.801482E-716.8181821
CCGCCGG1005.874019E-616.6513
TGGACCC5200.016.3653855
GTACTGA1251.6560443E-716.2799996
AGGAGTG1059.337058E-615.8571425
TCTAATA1059.337058E-615.8571422
GTCTTAT700.002591718515.8571421
Produced by FastQC (version 0.11.5)