##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840900
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76055178974908	31.0	31.0	34.0	30.0	34.0
2	31.91782257105482	31.0	31.0	34.0	30.0	34.0
3	32.03264954215721	33.0	31.0	34.0	30.0	34.0
4	35.6975288381496	37.0	35.0	37.0	35.0	37.0
5	35.61922582946843	37.0	35.0	37.0	33.0	37.0
6	35.681392555595195	37.0	35.0	37.0	33.0	37.0
7	35.5196230229516	37.0	35.0	37.0	33.0	37.0
8	35.574083719823996	37.0	35.0	37.0	33.0	37.0
9	37.17099060530384	39.0	37.0	39.0	33.0	39.0
10	37.05209894161018	39.0	37.0	39.0	33.0	39.0
11	37.10962540135569	39.0	37.0	39.0	33.0	39.0
12	36.96223332144131	39.0	37.0	39.0	33.0	39.0
13	37.01142585325247	39.0	37.0	39.0	33.0	39.0
14	38.01058270900226	40.0	37.0	41.0	33.0	41.0
15	38.022207158996316	40.0	37.0	41.0	33.0	41.0
16	37.92250683791176	40.0	37.0	41.0	33.0	41.0
17	37.99192769651564	40.0	37.0	41.0	33.0	41.0
18	37.96696515638007	40.0	37.0	41.0	33.0	41.0
19	38.04213580687359	40.0	37.0	41.0	33.0	41.0
20	37.94184445237246	40.0	37.0	41.0	33.0	41.0
21	37.8188559876323	40.0	37.0	41.0	32.0	41.0
22	37.71208823879177	40.0	37.0	41.0	32.0	41.0
23	37.523320252110835	39.0	36.0	41.0	32.0	41.0
24	37.65766321798073	40.0	37.0	41.0	32.0	41.0
25	37.605465572600785	40.0	37.0	41.0	32.0	41.0
26	37.398559876322985	39.0	36.0	41.0	32.0	41.0
27	37.35764775835415	39.0	36.0	41.0	32.0	41.0
28	37.17933285765252	39.0	36.0	41.0	31.0	41.0
29	36.93874895944821	39.0	35.0	41.0	31.0	41.0
30	36.75534784159829	39.0	35.0	41.0	30.0	41.0
31	36.4824711618504	39.0	35.0	40.0	30.0	41.0
32	36.31937804733024	38.0	35.0	40.0	30.0	41.0
33	36.13704602211916	38.0	35.0	40.0	30.0	41.0
34	35.914144369128316	38.0	35.0	40.0	29.0	41.0
35	35.71339398263765	38.0	35.0	40.0	28.0	41.0
36	35.62349387560947	38.0	35.0	40.0	27.0	41.0
37	35.452352241645855	38.0	35.0	40.0	26.0	41.0
38	35.29619217505054	38.0	34.0	40.0	25.0	41.0
39	35.127905815198005	38.0	34.0	40.0	24.0	41.0
40	34.91367106671424	38.0	34.0	40.0	24.0	41.0
41	34.751335473897015	38.0	34.0	40.0	23.0	41.0
42	34.42278154358426	38.0	33.0	40.0	21.0	41.0
43	34.02302057319539	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	2.0
15	3.0
16	6.0
17	20.0
18	38.0
19	117.0
20	246.0
21	538.0
22	1010.0
23	1873.0
24	2881.0
25	4516.0
26	6724.0
27	9434.0
28	13118.0
29	17244.0
30	21822.0
31	26208.0
32	32600.0
33	40208.0
34	50414.0
35	62196.0
36	81960.0
37	122053.0
38	154926.0
39	190740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.263408253062195	21.14389344749673	15.590557735759306	22.002140563681767
2	18.28647877274349	24.168747770246167	36.127839219883455	21.41693423712689
3	19.129028421928886	24.311333095492923	34.20275894874539	22.3568795338328
4	13.293257224402428	16.302176239743133	35.86776073254846	34.53680580330598
5	11.885717683434416	39.23891069092639	34.68605066000713	14.18932096563206
6	31.571768343441555	38.12248781067903	13.19062908788203	17.115114757997386
7	24.48602687596623	34.896777262456894	20.96848614579617	19.64870971578071
8	27.31799262694732	34.56213580687359	18.814841241526935	19.305030324652158
9	24.119039124747292	14.869544535616603	22.372339160423355	38.63907717921275
10	16.445712926626232	28.660244975621357	32.29944107503865	22.59460102271376
11	32.75740278273279	23.026400285408492	23.624568914258532	20.59162801760019
12	21.60756332500892	28.55012486621477	30.46283743608039	19.37947437269592
13	32.48531335473897	21.585325246759425	26.15388274467832	19.775478653823285
14	20.0172434296587	23.736234986324174	28.93245332381972	27.314068260197406
15	21.79605184920918	30.893923177547865	25.97371863479605	21.336306338446903
16	20.823165655844928	29.043405874658106	26.84576049470805	23.287667974788917
17	21.203234629563564	27.50909739564752	27.3707931977643	23.916874777024617
18	22.82792246402664	25.65560708764419	30.706742775597572	20.809727672731597
19	22.441075038649068	25.046022119158046	32.11452015697467	20.39838268521822
20	23.215364490426925	24.674515400166488	31.506600071352125	20.603520038054466
21	21.909977405161136	25.651088119871567	32.442502081103584	19.996432393863717
22	20.32714948269711	26.09561184445237	31.79521940777738	21.782019265073135
23	21.72125104055179	26.141633963610417	32.48424307289808	19.652871922939706
24	21.152336782019265	26.233321441312878	30.4815079081936	22.132833868474254
25	21.08169818052087	26.586276608395764	31.724105125460817	20.607920085622546
26	20.428945177785707	27.761327149482696	31.68391009632537	20.12581757640623
27	19.40789630158164	27.451777857057913	31.34011178499227	21.800214056368176
28	20.28053276251635	26.985610655250326	31.188845284813887	21.545011297419432
29	20.253062195266978	26.850992983707933	30.39457723867285	22.50136758235224
30	20.471280770602927	27.24628374360804	32.59281721964562	19.68961826614342
31	21.568081817100726	26.888215007729816	31.980259246045904	19.563443929123558
32	20.738018789392317	26.878701391366395	30.954691402069212	21.42858841717208
33	20.25234867403972	26.459507670353194	31.881912236889047	21.40623141871804
34	19.298014032584135	27.018075871090495	32.80104649779997	20.88286359852539
35	18.746699964323938	29.210964442858845	31.371506718991554	20.670828873825663
36	21.325722440242597	26.59460102271376	31.284813889879892	20.794862647163754
37	20.2739921512665	27.72826733261981	31.113568795338324	20.88417172077536
38	19.883696039957186	25.841955048162685	32.84136044713997	21.43298846474016
39	19.444166963967177	25.594838863122842	33.59020097514568	21.3707931977643
40	17.949696753478417	25.991675585682007	33.57723867285052	22.48138898798906
41	19.17588298251873	25.278630039243666	33.81983589011773	21.72565108811987
42	18.319538589606374	27.31965750981092	32.78725175407302	21.57355214650969
43	18.23510524438102	27.372814841241528	32.03769770484005	22.354382209537402
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	327.0
1	318.0
2	309.0
3	660.0
4	1011.0
5	1011.0
6	1557.0
7	2103.0
8	2283.0
9	2463.0
10	3692.5
11	4922.0
12	4922.0
13	8651.5
14	12381.0
15	21680.5
16	30980.0
17	28153.5
18	25327.0
19	25327.0
20	24904.0
21	24481.0
22	19290.0
23	14099.0
24	15325.5
25	16552.0
26	16552.0
27	17664.0
28	18776.0
29	19642.5
30	20509.0
31	22258.5
32	24008.0
33	24008.0
34	26459.0
35	28910.0
36	31274.0
37	33638.0
38	36086.5
39	38535.0
40	38535.0
41	39812.5
42	41090.0
43	43758.0
44	46426.0
45	51614.5
46	56803.0
47	56803.0
48	76783.5
49	96764.0
50	87757.0
51	78750.0
52	69857.0
53	60964.0
54	60964.0
55	53399.0
56	45834.0
57	38993.5
58	32153.0
59	28476.0
60	24799.0
61	24799.0
62	21604.5
63	18410.0
64	15893.5
65	13377.0
66	11276.5
67	9176.0
68	9176.0
69	7741.5
70	6307.0
71	5360.0
72	4413.0
73	3583.5
74	2754.0
75	2754.0
76	2170.5
77	1587.0
78	1236.5
79	886.0
80	706.0
81	526.0
82	526.0
83	397.0
84	268.0
85	209.5
86	151.0
87	108.0
88	65.0
89	65.0
90	57.0
91	49.0
92	30.0
93	11.0
94	7.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	840900.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.68596441364811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.44889133924808	47.87066849228652
2	8.928760358612523	10.122707838926027
3	2.5830346035321137	4.392654228451291
4	1.165388094677433	2.6424459225189656
5	0.6623058179256281	1.8771722012942131
6	0.4338113346829243	1.475460832804406
7	0.30928427051327206	1.227245400641152
8	0.22530588048855302	1.0217344898847815
9	0.16804531430080824	0.8573229635702334
>10	0.9570482585527414	9.881839629959671
>50	0.06515825198625855	2.5779753966986294
>100	0.041616516454797046	4.576242962585604
>500	0.0073564549235950495	2.92465401231645
>1k	0.00336295082221488	3.8387349828013075
>5k	0.0	0.0
>10k+	6.3055327916529E-4	4.7131406452606885
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16471	1.958734689023665	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11719	1.3936258770365084	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11368	1.3518848852420027	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4526	0.5382328457604948	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3231	0.38423118087763114	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2661	0.31644666428826257	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2660	0.31632774408371983	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2556	0.30396004281127365	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2361	0.28077060292543704	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2120	0.25211083363063386	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1919	0.22820787251754074	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1614	0.19193721013200143	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1522	0.18099655131406828	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1274	0.1515043405874658	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1241	0.147579973837555	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1236	0.14698537281484123	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1220	0.14508264954215722	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1054	0.12534189558806041	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1024	0.12177428945177786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.11761208229278154	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	977	0.11618503983826853	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	966	0.11487691758829824	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	933	0.11095255083838744	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	926	0.11012010940658819	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.10655250327030563	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	886	0.1053633012248781	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	878	0.1044119395885361	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	867	0.10310381733856583	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	854	0.10155785467951006	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1892020454275182E-4	0.0
9	0.0	0.0	0.0	2.3784040908550363E-4	0.0
10	0.0	0.0	0.0	3.5676061362825543E-4	0.0
11	0.0	0.0	0.0	0.0011892020454275182	0.0
12	0.0	0.0	0.0	0.001902723272684029	0.0
13	0.0	0.0	0.0	0.0022594838863122845	0.0
14	1.1892020454275182E-4	0.0	0.0	0.0022594838863122845	0.0
15	1.1892020454275182E-4	0.0	0.0	0.0029730051135687953	0.0
16	1.1892020454275182E-4	0.0	0.0	0.00392436674991081	0.0
17	1.1892020454275182E-4	0.0	0.0	0.005351409204423832	0.0
18	1.1892020454275182E-4	0.0	0.0	0.006421691045308598	0.0
19	1.1892020454275182E-4	0.0	0.0	0.008205494113449875	0.0
20	1.1892020454275182E-4	0.0	0.0	0.015935307408728745	0.0
21	1.1892020454275182E-4	0.0	0.0	0.025686764181234393	0.0
22	1.1892020454275182E-4	0.0	0.0	0.041027470567249376	0.0
23	1.1892020454275182E-4	0.0	0.0	0.05066000713521227	0.0
24	1.1892020454275182E-4	0.0	0.0	0.06314662861220122	0.0
25	1.1892020454275182E-4	0.0	0.0	0.06814127720299679	0.0
26	1.1892020454275182E-4	0.0	0.0	0.07908193602092996	0.0
27	1.1892020454275182E-4	0.0	0.0	0.15186110120109406	0.0
28	1.1892020454275182E-4	0.0	0.0	0.33773338090141514	0.0
29	1.1892020454275182E-4	0.0	0.0	0.6026875966226662	0.0
30	1.1892020454275182E-4	0.0	0.0	1.003805446545368	0.0
31	1.1892020454275182E-4	0.0	0.0	1.5684385777143537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACCGT	25	0.005495516	29.599998	6
ATTGGAC	295	0.0	24.457626	3
GGTATCA	4290	0.0	22.76923	1
TATTGGA	330	0.0	22.424242	2
GTATTGG	345	0.0	21.985508	1
CGAACGT	65	6.899316E-5	19.923077	12
GGACCCT	420	0.0	19.821428	6
TTGGACC	470	0.0	18.5	4
CGCCGGA	90	2.1508185E-6	18.5	14
GTGTATG	75	2.0664357E-4	17.266666	1
ACTATAG	65	0.0015794575	17.076923	3
CCGCTAT	65	0.0015794575	17.076923	25
GACCCTC	490	0.0	16.989798	7
CTCTAAT	110	7.801482E-7	16.818182	1
CCGCCGG	100	5.874019E-6	16.65	13
TGGACCC	520	0.0	16.365385	5
GTACTGA	125	1.6560443E-7	16.279999	6
AGGAGTG	105	9.337058E-6	15.857142	5
TCTAATA	105	9.337058E-6	15.857142	2
GTCTTAT	70	0.0025917185	15.857142	1
>>END_MODULE