FastQCFastQC Report
Wed 25 May 2016
SRR2088530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497484
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT514361.4706571924274707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT388431.1105983615650565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT374541.070884098397591No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144250.41243934210992816No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95950.27434006846064196No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG91330.2611305727202755No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75290.21526903339657877No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74880.2140967621295766No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA67720.1936249029302207No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67090.19182360805653434No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59250.1694074940728821No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC51330.1467626442322538No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46390.13263820506398313No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.12569035340833581No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39810.11382468082770357No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA38210.10924996368818271No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT38150.10907841179545066No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT36620.10470383853078385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130850.021.8295761
GTATTGG9800.021.7091831
TATACCG2050.019.853665
ATTGGAC10600.018.8490543
GGACCCT13900.017.3021586
TTGGACC15200.016.6743434
GACCCTC14700.016.2346947
TGGACCC15600.015.89102655
CCGTATA2300.015.2826092
AACGGTA1406.004466E-714.53571418
ACGTTCG3650.014.19178122
TAGTACT5250.014.0952384
GTATTAG6350.013.9842521
TATACAC14050.013.82562337
TATTGGA13950.013.7921142
GTATCAA206850.013.782212
CCCTCGC16300.013.73312959
ACCCTCG17000.013.4941188
CTAGTAC4450.013.3033713
CTAATAC6450.013.1937993
Produced by FastQC (version 0.11.5)