##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3497484
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807506767722167	31.0	31.0	34.0	30.0	34.0
2	31.97300916887683	31.0	31.0	34.0	30.0	34.0
3	32.09708407529527	33.0	31.0	34.0	30.0	34.0
4	35.7546687847607	37.0	35.0	37.0	35.0	37.0
5	35.66449996626146	37.0	35.0	37.0	33.0	37.0
6	35.70923440964991	37.0	35.0	37.0	33.0	37.0
7	35.57624366544636	37.0	35.0	37.0	33.0	37.0
8	35.606602632063506	37.0	35.0	37.0	33.0	37.0
9	37.229839507485956	39.0	37.0	39.0	34.0	39.0
10	37.095321093677626	39.0	37.0	39.0	33.0	39.0
11	37.15173393216381	39.0	37.0	39.0	33.0	39.0
12	37.0118450863535	39.0	37.0	39.0	33.0	39.0
13	37.07090068174722	39.0	37.0	39.0	33.0	39.0
14	38.13182676461136	40.0	37.0	41.0	33.0	41.0
15	38.127452763186334	40.0	37.0	41.0	33.0	41.0
16	38.019616673014085	40.0	37.0	41.0	33.0	41.0
17	38.04682937791853	40.0	37.0	41.0	33.0	41.0
18	38.01101248783411	40.0	37.0	41.0	33.0	41.0
19	38.07170897708181	40.0	37.0	41.0	33.0	41.0
20	37.972869925923895	40.0	37.0	41.0	33.0	41.0
21	37.84441901664168	40.0	37.0	41.0	32.0	41.0
22	37.72911984729594	40.0	37.0	41.0	32.0	41.0
23	37.58387686691347	40.0	37.0	41.0	32.0	41.0
24	37.741153640731454	40.0	37.0	41.0	32.0	41.0
25	37.702606788194025	40.0	37.0	41.0	32.0	41.0
26	37.521179224837056	40.0	37.0	41.0	32.0	41.0
27	37.48051799522171	40.0	36.0	41.0	32.0	41.0
28	37.33685272041273	39.0	36.0	41.0	31.0	41.0
29	37.12438112654697	39.0	36.0	41.0	31.0	41.0
30	36.98122250166119	39.0	36.0	41.0	31.0	41.0
31	36.77316293655668	39.0	35.0	41.0	30.0	41.0
32	36.63601720551116	39.0	35.0	40.0	30.0	41.0
33	36.480484542602625	39.0	35.0	40.0	30.0	41.0
34	36.30880284227176	38.0	35.0	40.0	30.0	41.0
35	36.11463669311997	38.0	35.0	40.0	30.0	41.0
36	36.04515789064367	38.0	35.0	40.0	29.0	41.0
37	35.92348957136044	38.0	35.0	40.0	28.0	41.0
38	35.788624908648615	38.0	35.0	40.0	27.0	41.0
39	35.65737970495362	38.0	35.0	40.0	27.0	41.0
40	35.480555450718285	38.0	35.0	40.0	26.0	41.0
41	35.3493980244084	38.0	35.0	40.0	25.0	41.0
42	35.07284407877206	38.0	34.0	40.0	24.0	41.0
43	34.69487008375163	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	7.0
15	16.0
16	34.0
17	82.0
18	205.0
19	468.0
20	945.0
21	1959.0
22	3659.0
23	6444.0
24	10626.0
25	16451.0
26	24251.0
27	34710.0
28	47997.0
29	64240.0
30	81874.0
31	102451.0
32	127214.0
33	158537.0
34	199660.0
35	251558.0
36	334829.0
37	493728.0
38	669503.0
39	866030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44324891836531	21.015907435173396	14.37713510626496	23.163708540196325
2	18.263929155930377	23.639078834956788	35.80839826572473	22.288593743388105
3	18.74627589432861	24.35390697998904	33.76730243798114	23.132514687701217
4	13.180732206351767	16.29631472224033	36.80288458789233	33.72006848351558
5	12.316024891035957	38.63914745571388	35.240389948888975	13.804437704361192
6	31.75914457364208	38.19622906066189	13.874573836506471	16.170052529189554
7	25.68351992460866	33.96776082463851	21.32515831380501	19.023560936947817
8	27.20921668262099	34.36407428883163	19.104762166174314	19.321946862373064
9	24.634851796319868	14.635520848701525	21.80195820767157	38.927669147307036
10	16.234470264910435	28.24044370181536	33.13756403174396	22.387522001530243
11	33.26399777668747	22.94294984623232	23.403137798486	20.38991457859421
12	21.590749235736318	27.76595975850068	30.50264132730843	20.14064967845457
13	31.89515663259646	21.192891804508612	25.778130793450377	21.13382076944455
14	20.61742098033901	23.07953374482914	28.280329516875558	28.02271575795629
15	22.876244751941684	30.3838416415915	24.842429586525625	21.897484019941192
16	21.76570357434087	28.57305422984065	26.35883395034831	23.302408245470172
17	21.68501700079257	27.4366087164373	27.00644234541173	23.871931937358397
18	22.766766052396523	25.89247012995628	29.70152258023196	21.639241237415238
19	22.6266653399987	25.230108272117903	31.104788470797867	21.03843791708554
20	23.508070372873757	25.025990111748904	30.20634261657809	21.259596898799252
21	22.334054994962095	25.79763052525759	30.887660958563355	20.980653521216965
22	21.30497237442687	26.415846362699586	30.34149691606881	21.93768434680473
23	22.25925836973093	26.24054892030957	30.741127050188076	20.759065659771426
24	21.702200782047896	26.479463522921048	29.335287881231192	22.483047813799864
25	22.015454538176588	26.272657716232583	30.328316012310562	21.383571733280267
26	21.258653363389225	27.485300861991075	30.288258645357637	20.967787129262064
27	20.432516631956	27.3008253933399	30.096034749551393	22.17062322515271
28	21.115121613136758	27.20418449376752	29.850458215105487	21.830235677990235
29	21.207788227194175	27.145456562488924	29.156530809004416	22.490224401312485
30	21.12921746032291	27.59269234684133	30.76826084122186	20.509829351613902
31	22.038757003606023	27.238695016188778	30.363055270588802	20.359492709616397
32	21.352606616642134	27.166586037277085	29.484137740158356	21.996669605922428
33	20.968987992511188	27.01267539751433	30.288515973196734	21.729820636777752
34	20.464225140129304	27.437752395722182	30.84111321166873	21.256909252479783
35	19.953829667269385	29.507440205587788	29.807827569761574	20.73090255738125
36	21.966962536497665	27.688475486950047	29.49380183011559	20.850760146436695
37	20.9905749390133	28.288649783673065	29.492858294705567	21.22791698260807
38	20.706713740506032	26.979422922306433	30.739068427475292	21.57479490971224
39	20.318577583199808	26.659821746146662	31.44225963578389	21.579341034869636
40	19.245377534250334	27.012046373907644	31.52031574697697	22.222260344865052
41	20.370357662822762	26.08395063422735	31.768322599903243	21.77736910304665
42	19.44031766835817	27.704429812974123	31.04511700410924	21.810135514558464
43	19.374699069388164	27.471776854447366	30.69109108147457	22.462432994689895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1722.0
1	1397.0
2	1072.0
3	2343.0
4	3614.0
5	3614.0
6	5104.5
7	6595.0
8	7194.0
9	7793.0
10	11575.0
11	15357.0
12	15357.0
13	27176.0
14	38995.0
15	67592.5
16	96190.0
17	88902.0
18	81614.0
19	81614.0
20	83446.5
21	85279.0
22	68557.0
23	51835.0
24	58991.0
25	66147.0
26	66147.0
27	73809.0
28	81471.0
29	87009.5
30	92548.0
31	100823.0
32	109098.0
33	109098.0
34	122867.0
35	136636.0
36	148230.0
37	159824.0
38	168501.5
39	177179.0
40	177179.0
41	183808.0
42	190437.0
43	199973.0
44	209509.0
45	221135.0
46	232761.0
47	232761.0
48	292153.0
49	351545.0
50	329144.0
51	306743.0
52	278422.0
53	250101.0
54	250101.0
55	225276.0
56	200451.0
57	171789.0
58	143127.0
59	129551.5
60	115976.0
61	115976.0
62	102329.5
63	88683.0
64	77042.0
65	65401.0
66	55456.0
67	45511.0
68	45511.0
69	38456.5
70	31402.0
71	26414.0
72	21426.0
73	17579.0
74	13732.0
75	13732.0
76	10927.5
77	8123.0
78	6309.0
79	4495.0
80	3513.0
81	2531.0
82	2531.0
83	1894.5
84	1258.0
85	942.5
86	627.0
87	488.0
88	349.0
89	349.0
90	278.5
91	208.0
92	144.5
93	81.0
94	54.0
95	27.0
96	27.0
97	16.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3497484.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.319151608084574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67026754516813	33.093229079505434
2	13.15925874281209	11.664143665454029
3	4.88629127788694	6.496688518377979
4	2.2680897560689215	4.020792550398484
5	1.2749977191705821	2.825340860794154
6	0.8004600432693484	2.128542600832091
7	0.5191112773581904	1.6104599981892855
8	0.35904789854834807	1.2730158600262724
9	0.25904519740684484	1.033259703949814
>10	1.5650275219984595	12.897836574157514
>50	0.14055090104311418	4.32203227989476
>100	0.08686998272552904	7.1541315539347154
>500	0.005878673091534967	1.791268978707004
>1k	0.004328253813631912	3.950497501410835
>5k	5.168064255082879E-4	1.6687016884247883
>10k+	2.5840321275414393E-4	4.0700585859428156
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	51436	1.4706571924274707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38843	1.1105983615650565	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	37454	1.070884098397591	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14425	0.41243934210992816	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9595	0.27434006846064196	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9133	0.2611305727202755	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7529	0.21526903339657877	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7488	0.2140967621295766	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6772	0.1936249029302207	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6709	0.19182360805653434	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5925	0.1694074940728821	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5133	0.1467626442322538	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4639	0.13263820506398313	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4396	0.12569035340833581	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3981	0.11382468082770357	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3821	0.10924996368818271	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3815	0.10907841179545066	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3662	0.10470383853078385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.859198212200542E-5
2	0.0	0.0	0.0	0.0	2.859198212200542E-5
3	0.0	0.0	0.0	0.0	2.859198212200542E-5
4	0.0	0.0	0.0	0.0	2.859198212200542E-5
5	0.0	0.0	0.0	0.0	2.859198212200542E-5
6	2.859198212200542E-5	0.0	0.0	0.0	2.859198212200542E-5
7	2.859198212200542E-5	0.0	0.0	0.0	2.859198212200542E-5
8	2.859198212200542E-5	0.0	0.0	0.0	2.859198212200542E-5
9	2.859198212200542E-5	0.0	0.0	5.718396424401084E-5	2.859198212200542E-5
10	2.859198212200542E-5	0.0	0.0	8.577594636601626E-5	2.859198212200542E-5
11	2.859198212200542E-5	0.0	0.0	1.1436792848802168E-4	5.718396424401084E-5
12	2.859198212200542E-5	0.0	0.0	1.429599106100271E-4	5.718396424401084E-5
13	2.859198212200542E-5	0.0	0.0	1.7155189273203252E-4	5.718396424401084E-5
14	2.859198212200542E-5	0.0	0.0	1.7155189273203252E-4	5.718396424401084E-5
15	2.859198212200542E-5	0.0	0.0	3.145118033420596E-4	5.718396424401084E-5
16	2.859198212200542E-5	0.0	0.0	6.004316245621138E-4	5.718396424401084E-5
17	2.859198212200542E-5	0.0	0.0	0.001029311356392195	5.718396424401084E-5
18	2.859198212200542E-5	0.0	0.0	0.0012008632491242277	5.718396424401084E-5
19	2.859198212200542E-5	0.0	0.0	0.0015153750524662872	5.718396424401084E-5
20	5.718396424401084E-5	0.0	0.0	0.002516094426736477	5.718396424401084E-5
21	8.577594636601626E-5	0.0	0.0	0.00443175722891084	8.577594636601626E-5
22	1.1436792848802168E-4	0.0	0.0	0.007290955441111382	8.577594636601626E-5
23	1.1436792848802168E-4	0.0	0.0	0.008920698422065691	8.577594636601626E-5
24	1.1436792848802168E-4	0.0	0.0	0.011007913116972086	8.577594636601626E-5
25	1.1436792848802168E-4	0.0	0.0	0.013152311776122493	8.577594636601626E-5
26	1.1436792848802168E-4	0.0	0.0	0.01789858080837539	1.1436792848802168E-4
27	1.1436792848802168E-4	0.0	0.0	0.05097950412353566	1.1436792848802168E-4
28	1.1436792848802168E-4	0.0	0.0	0.1397862005944845	1.1436792848802168E-4
29	1.1436792848802168E-4	0.0	0.0	0.2919241374656753	1.1436792848802168E-4
30	1.1436792848802168E-4	0.0	0.0	0.49787218469048033	1.1436792848802168E-4
31	1.1436792848802168E-4	0.0	0.0	0.7740993239711748	1.429599106100271E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13085	0.0	21.829576	1
GTATTGG	980	0.0	21.709183	1
TATACCG	205	0.0	19.85366	5
ATTGGAC	1060	0.0	18.849054	3
GGACCCT	1390	0.0	17.302158	6
TTGGACC	1520	0.0	16.674343	4
GACCCTC	1470	0.0	16.234694	7
TGGACCC	1560	0.0	15.8910265	5
CCGTATA	230	0.0	15.282609	2
AACGGTA	140	6.004466E-7	14.535714	18
ACGTTCG	365	0.0	14.191781	22
TAGTACT	525	0.0	14.095238	4
GTATTAG	635	0.0	13.984252	1
TATACAC	1405	0.0	13.825623	37
TATTGGA	1395	0.0	13.792114	2
GTATCAA	20685	0.0	13.78221	2
CCCTCGC	1630	0.0	13.7331295	9
ACCCTCG	1700	0.0	13.494118	8
CTAGTAC	445	0.0	13.303371	3
CTAATAC	645	0.0	13.193799	3
>>END_MODULE