FastQCFastQC Report
Wed 25 May 2016
SRR2088529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2256838
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270861.2001747577805761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT199720.8849549679684585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196410.8702884300955585No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.3575356317112704No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG45410.20121072048591876No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43340.1920385955925946No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38450.17037111214894465No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG37510.16620599263216942No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36470.16159777529446065No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32920.14586780265132013No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA31690.1404176994538376No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.11104031392594417No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC24200.10722967266591577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.022.2410771
TATAGCG450.003826932720.5555572
ATTGGAC6850.018.6350363
GTATTGG7150.018.6293721
GGACCCT9950.016.9195986
TATACCG1251.6596823E-716.2799995
ACTAGAC1059.350999E-615.8571423
TATACTG3400.015.7794125
TGGACCC10550.015.6066355
TATTGGA8300.015.37951852
GACCCTC9800.015.2908157
TTGGACC9600.015.2239584
CGAACTA1101.4530224E-515.13636424
GACCGTG2109.094947E-1214.976197
TATTACG1051.6567466E-414.0952382
TCTATAC2500.014.0599993
GTACCGA800.006301100413.8750016
ACGTTTA1356.5732092E-613.70370426
GTATCAA103500.013.6024151
GTATTAG4100.013.5365861
Produced by FastQC (version 0.11.5)