##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2256838
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78313374730486	31.0	31.0	34.0	30.0	34.0
2	31.957825063207903	31.0	31.0	34.0	30.0	34.0
3	32.089357765156386	33.0	31.0	34.0	30.0	34.0
4	35.750774756539904	37.0	35.0	37.0	35.0	37.0
5	35.650164079123094	37.0	35.0	37.0	33.0	37.0
6	35.69116259120061	37.0	35.0	37.0	33.0	37.0
7	35.55731115835518	37.0	35.0	37.0	33.0	37.0
8	35.57750578464205	37.0	35.0	37.0	33.0	37.0
9	37.20912710615472	39.0	37.0	39.0	33.0	39.0
10	37.06168763553254	39.0	37.0	39.0	33.0	39.0
11	37.12000728452818	39.0	37.0	39.0	33.0	39.0
12	36.97570760506514	39.0	37.0	39.0	33.0	39.0
13	37.02522866063049	39.0	37.0	39.0	33.0	39.0
14	38.10103073415105	40.0	37.0	41.0	33.0	41.0
15	38.100104216607484	40.0	37.0	41.0	33.0	41.0
16	38.00143342145072	40.0	37.0	41.0	33.0	41.0
17	38.000767888523676	40.0	37.0	41.0	33.0	41.0
18	37.95471185791803	40.0	37.0	41.0	33.0	41.0
19	38.00323328479935	40.0	37.0	41.0	33.0	41.0
20	37.890886275399474	40.0	37.0	41.0	32.0	41.0
21	37.758061057107334	40.0	37.0	41.0	32.0	41.0
22	37.63294662709508	40.0	37.0	41.0	32.0	41.0
23	37.46397570406028	39.0	36.0	41.0	32.0	41.0
24	37.63469996517251	39.0	37.0	41.0	32.0	41.0
25	37.59992697747911	40.0	37.0	41.0	32.0	41.0
26	37.41305756106553	39.0	36.0	41.0	32.0	41.0
27	37.365945185254766	39.0	36.0	41.0	31.0	41.0
28	37.22729455991081	39.0	36.0	41.0	31.0	41.0
29	37.013605318591765	39.0	36.0	41.0	31.0	41.0
30	36.89601424648114	39.0	36.0	41.0	30.0	41.0
31	36.69501178197106	39.0	35.0	41.0	30.0	41.0
32	36.55485949811196	39.0	35.0	40.0	30.0	41.0
33	36.39656058609435	39.0	35.0	40.0	30.0	41.0
34	36.21076701118999	38.0	35.0	40.0	30.0	41.0
35	36.03008944372613	38.0	35.0	40.0	29.0	41.0
36	35.99138130428502	38.0	35.0	40.0	29.0	41.0
37	35.876789561324294	38.0	35.0	40.0	28.0	41.0
38	35.75898092818359	38.0	35.0	40.0	27.0	41.0
39	35.64053733586549	38.0	35.0	40.0	26.0	41.0
40	35.48775454862068	38.0	35.0	40.0	26.0	41.0
41	35.3942609084037	38.0	35.0	40.0	25.0	41.0
42	35.143663390992174	38.0	34.0	40.0	24.0	41.0
43	34.78856346800258	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	5.0
15	6.0
16	34.0
17	57.0
18	123.0
19	320.0
20	737.0
21	1379.0
22	2689.0
23	4668.0
24	7377.0
25	11382.0
26	16496.0
27	23079.0
28	31902.0
29	42165.0
30	53845.0
31	67480.0
32	83239.0
33	102942.0
34	129140.0
35	163609.0
36	215132.0
37	312807.0
38	438875.0
39	547347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61167084212513	20.621506727554216	13.85544731168121	23.91137511863944
2	18.403359035960936	23.01986230292117	35.65160636253023	22.925172298587672
3	18.97912034448197	24.17351178950372	32.726363168291215	24.121004697723098
4	13.56331291833973	16.255885446806552	36.386927196369435	33.79387443848428
5	12.713008199968273	38.22144079459846	35.24338034010416	13.822170665329104
6	32.419473617512644	37.78308412034892	14.223572981312794	15.573869280825651
7	26.441286437041562	33.18231082603182	21.46135433735164	18.915048399574978
8	27.029454484548737	34.70395305289968	19.298372324464584	18.968220138087005
9	25.127102609934788	14.583722890167572	21.28327332311845	39.0059011767792
10	16.09933898667073	28.03940734780255	33.40523333974348	22.45602032578324
11	33.85621830188963	22.679253007969557	23.104228128026914	20.360300562113895
12	21.32656398022366	27.076378543785594	30.861009961725212	20.736047514265536
13	31.3457589778265	21.05534380403024	25.731443727906033	21.867453490237228
14	20.811640002516796	22.375996859322644	27.837399051238947	28.974964086921613
15	22.952422814575083	29.786630675307663	24.66114094144108	22.599805568676175
16	22.23389538814926	28.26658360059517	25.932388589699396	23.567132421556177
17	21.934760049237028	27.18662128163386	27.003134473985284	23.875484195143827
18	22.92455196163836	25.93340771468754	29.06482432500693	22.077215998667164
19	23.03036372127729	25.334295151003307	30.140843073361935	21.494498054357468
20	23.672899871413012	25.273369200624945	29.493034059157104	21.56069686880494
21	22.82445616388948	25.656294337475703	29.926738206286853	21.592511292347965
22	22.015536781993212	26.15088012520172	29.469416945301347	22.36416614750372
23	22.57069404184084	26.198380211605794	29.843037027912505	21.38788871864086
24	22.268324088835794	26.15641884796339	28.718897856204123	22.856359206996693
25	22.46443918438098	26.099658017101802	29.556973074717813	21.878929723799402
26	21.966663092344245	26.930200572659622	29.473537755036027	21.629598579960106
27	21.232317073711098	26.93263761067476	29.359971783530764	22.475073532083385
28	21.81946599623012	26.568942919252514	29.23417631216773	22.37741477234963
29	21.816851719086618	26.660664168185754	28.77096185016381	22.751522262563817
30	21.90804125063474	26.94920946917767	29.782997273176008	21.35975200701158
31	22.461647668109098	26.708474423064484	29.61311356863009	21.216764340196328
32	21.906711957171936	26.68454714073407	29.000885309446222	22.407855592647767
33	21.69048022055637	26.422720638344444	29.646390214982198	22.240408926116984
34	21.242862801849313	26.68809192330154	30.124138285512743	21.944906989336406
35	20.912444756779173	28.050972200928907	29.378227413753226	21.65835562853869
36	22.38317504402177	26.572044603999046	29.14604415558405	21.898736196395134
37	21.59610038469753	26.89975975236149	29.364491381304287	22.139648481636698
38	21.301174475084167	25.996416224824287	30.38530014117097	22.31710915892058
39	20.99508250038328	25.683722092591495	31.014233188204027	22.306962218821198
40	20.197772281395473	25.927292964758657	30.9911034819513	22.88383127189457
41	20.864191404079513	25.137028001123696	31.30915909781739	22.689621496979402
42	20.049954848332046	26.48289332242722	30.775093294246197	22.692058534994537
43	19.950390767968283	26.221731466768993	30.534269628568822	23.293608136693905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	756.0
1	677.0
2	598.0
3	1395.5
4	2193.0
5	2193.0
6	3070.0
7	3947.0
8	4158.5
9	4370.0
10	6510.0
11	8650.0
12	8650.0
13	15308.5
14	21967.0
15	36930.5
16	51894.0
17	48502.0
18	45110.0
19	45110.0
20	46327.5
21	47545.0
22	39728.5
23	31912.0
24	36088.0
25	40264.0
26	40264.0
27	45888.0
28	51512.0
29	56024.5
30	60537.0
31	66470.5
32	72404.0
33	72404.0
34	80105.5
35	87807.0
36	94937.5
37	102068.0
38	108528.5
39	114989.0
40	114989.0
41	119197.5
42	123406.0
43	129170.0
44	134934.0
45	140704.5
46	146475.0
47	146475.0
48	177229.5
49	207984.0
50	196934.0
51	185884.0
52	173029.0
53	160174.0
54	160174.0
55	147462.5
56	134751.0
57	119842.0
58	104933.0
59	95765.0
60	86597.0
61	86597.0
62	76528.5
63	66460.0
64	59004.5
65	51549.0
66	43792.0
67	36035.0
68	36035.0
69	30658.0
70	25281.0
71	21446.5
72	17612.0
73	14428.0
74	11244.0
75	11244.0
76	9063.5
77	6883.0
78	5324.0
79	3765.0
80	2949.0
81	2133.0
82	2133.0
83	1641.5
84	1150.0
85	847.5
86	545.0
87	399.0
88	253.0
89	253.0
90	208.5
91	164.0
92	114.0
93	64.0
94	45.5
95	27.0
96	27.0
97	18.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2256838.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.37107705825787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1367468597736	44.37257985570628
2	11.291548108219033	12.504503608144438
3	3.598143623568935	5.97699263541944
4	1.586462885880913	3.513766348167128
5	0.8363963434922217	2.315608319337646
6	0.5265529370074614	1.7493481950175285
7	0.34575958662762424	1.3401556500352696
8	0.25369885650051766	1.1238063146305655
9	0.19246635336252402	0.9591362354842385
>10	1.1043390791508452	11.37354113158755
>50	0.08007635170892204	3.0658289733610675
>100	0.041796398219464424	4.188128494456135
>500	0.002805887694376989	1.1333656531957041
>1k	0.0028860559142163	3.0642865582700933
>5k	8.016821983934167E-5	0.35818296473586336
>10k+	2.40504659518025E-4	2.9607690624510288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27086	1.2001747577805761	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19972	0.8849549679684585	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19641	0.8702884300955585	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8069	0.3575356317112704	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4541	0.20121072048591876	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4334	0.1920385955925946	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3845	0.17037111214894465	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3751	0.16620599263216942	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3647	0.16159777529446065	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3292	0.14586780265132013	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3169	0.1404176994538376	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2506	0.11104031392594417	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2420	0.10722967266591577	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.430978209335362E-5	0.0
4	0.0	0.0	0.0	4.430978209335362E-5	0.0
5	0.0	0.0	0.0	4.430978209335362E-5	0.0
6	4.430978209335362E-5	0.0	0.0	4.430978209335362E-5	0.0
7	4.430978209335362E-5	0.0	0.0	4.430978209335362E-5	0.0
8	1.3292934628006086E-4	0.0	0.0	4.430978209335362E-5	0.0
9	1.3292934628006086E-4	0.0	0.0	8.861956418670724E-5	0.0
10	1.3292934628006086E-4	0.0	0.0	1.3292934628006086E-4	0.0
11	1.3292934628006086E-4	0.0	0.0	2.215489104667681E-4	0.0
12	1.3292934628006086E-4	0.0	0.0	3.5447825674682896E-4	0.0
13	1.3292934628006086E-4	0.0	0.0	4.8740760302688985E-4	0.0
14	1.3292934628006086E-4	0.0	0.0	5.76027167213597E-4	0.0
15	1.3292934628006086E-4	0.0	0.0	6.203369493069507E-4	0.0
16	1.3292934628006086E-4	0.0	0.0	7.532662955870115E-4	0.0
17	1.3292934628006086E-4	0.0	0.0	0.0011077445523338405	0.0
18	1.7723912837341448E-4	0.0	0.0	0.0011963641165205479	0.0
19	1.7723912837341448E-4	0.0	0.0	0.001506532591174023	0.0
20	1.7723912837341448E-4	0.0	0.0	0.0026585869256012173	0.0
21	1.7723912837341448E-4	0.0	0.0	0.005583032543762557	0.0
22	1.7723912837341448E-4	0.0	0.0	0.008773336854484016	0.0
23	1.7723912837341448E-4	0.0	0.0	0.010368489009844748	0.0
24	1.7723912837341448E-4	0.0	0.0	0.013425863974286148	0.0
25	1.7723912837341448E-4	0.0	0.0	0.015597043296860475	0.0
26	1.7723912837341448E-4	0.0	0.0	0.02007233128828919	0.0
27	1.7723912837341448E-4	0.0	0.0	0.05662790151530593	0.0
28	1.7723912837341448E-4	0.0	0.0	0.1560147427506981	0.0
29	1.7723912837341448E-4	0.0	0.0	0.30737695838159407	0.0
30	1.7723912837341448E-4	0.0	0.0	0.5035363637088706	0.0
31	1.7723912837341448E-4	0.0	0.0	0.8186675339568015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6305	0.0	22.241077	1
TATAGCG	45	0.0038269327	20.555557	2
ATTGGAC	685	0.0	18.635036	3
GTATTGG	715	0.0	18.629372	1
GGACCCT	995	0.0	16.919598	6
TATACCG	125	1.6596823E-7	16.279999	5
ACTAGAC	105	9.350999E-6	15.857142	3
TATACTG	340	0.0	15.779412	5
TGGACCC	1055	0.0	15.606635	5
TATTGGA	830	0.0	15.3795185	2
GACCCTC	980	0.0	15.290815	7
TTGGACC	960	0.0	15.223958	4
CGAACTA	110	1.4530224E-5	15.136364	24
GACCGTG	210	9.094947E-12	14.97619	7
TATTACG	105	1.6567466E-4	14.095238	2
TCTATAC	250	0.0	14.059999	3
GTACCGA	80	0.0063011004	13.875001	6
ACGTTTA	135	6.5732092E-6	13.703704	26
GTATCAA	10350	0.0	13.602415	1
GTATTAG	410	0.0	13.536586	1
>>END_MODULE