FastQCFastQC Report
Wed 25 May 2016
SRR2088528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085133
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT561341.8195001641744455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT412411.3367657083179234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT408981.3256478732035215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160790.5211768828118593No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA113760.3687361290420867No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG111200.3604382695981016No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97330.3154807264386981No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG93440.30287186970545515No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA92960.30131602105970795No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA84700.27454245894747487No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA79250.25687709411555354No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC62890.20384858610633644No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57840.18747976181253773No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51240.16608684293351372No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.16479030239539105No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT42860.13892431865984384No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38450.12462995922704143No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37470.12145343490864088No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT37040.12005965383015903No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36880.11954103761490996No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.11792036194225662No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36160.11720726464628917No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG35040.11357695113954568No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG34470.11172938087272088No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA33180.10754803763727529No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT32740.10612184304534035No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT31540.10223222143097234No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31410.10181084575608247No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT31210.10116257548702114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133350.023.2099721
CCGTTTA2450.021.8979627
GTATTGG13050.021.2643681
ACCGTTA2400.020.8124988
ATTGGAC14200.019.4119723
GACCGTT2500.019.2400027
TTGGACC18950.019.1345654
TGTTACG1409.458745E-1118.516
GCCGTTT2800.018.526
GACCCTC19250.017.779227
GGACCCT19700.017.6548236
CCGTTAT2800.017.1785729
TGGACCC20200.017.1262365
GTTACGG3000.016.6517
GGACCGT3450.016.6231886
TATTGGA16400.016.4695112
ACGTTTA2250.016.44444526
CGTTTAC3050.016.3770528
TATACCG2150.016.3488375
CAATGCG1751.3278623E-1015.85714319
Produced by FastQC (version 0.11.5)