##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088528_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3085133
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763256235630685	31.0	31.0	34.0	30.0	34.0
2	31.923306385818698	31.0	31.0	34.0	30.0	34.0
3	32.036670056039725	33.0	31.0	34.0	30.0	34.0
4	35.697814972644615	37.0	35.0	37.0	35.0	37.0
5	35.62676779250684	37.0	35.0	37.0	33.0	37.0
6	35.68389369275166	37.0	35.0	37.0	33.0	37.0
7	35.52943422536403	37.0	35.0	37.0	33.0	37.0
8	35.57387671779466	37.0	35.0	37.0	33.0	37.0
9	37.17730548407476	39.0	37.0	39.0	33.0	39.0
10	37.05388325235897	39.0	37.0	39.0	33.0	39.0
11	37.11398600967932	39.0	37.0	39.0	33.0	39.0
12	36.96397140739151	39.0	37.0	39.0	33.0	39.0
13	37.01637174151001	39.0	37.0	39.0	33.0	39.0
14	38.026260132059136	40.0	37.0	41.0	33.0	41.0
15	38.02952482113413	40.0	37.0	41.0	33.0	41.0
16	37.92654157859645	40.0	37.0	41.0	33.0	41.0
17	37.99276497966214	40.0	37.0	41.0	33.0	41.0
18	37.97039057959576	40.0	37.0	41.0	33.0	41.0
19	38.042086030002594	40.0	37.0	41.0	33.0	41.0
20	37.94516314207524	40.0	37.0	41.0	33.0	41.0
21	37.821114681279546	40.0	37.0	41.0	32.0	41.0
22	37.70268737198688	40.0	37.0	41.0	32.0	41.0
23	37.52001032046268	39.0	36.0	41.0	32.0	41.0
24	37.66206546038696	40.0	37.0	41.0	32.0	41.0
25	37.62195697884013	40.0	37.0	41.0	32.0	41.0
26	37.41623586406161	39.0	36.0	41.0	32.0	41.0
27	37.379631283319064	39.0	36.0	41.0	32.0	41.0
28	37.22598442271371	39.0	36.0	41.0	31.0	41.0
29	37.00032219032372	39.0	36.0	41.0	31.0	41.0
30	36.834612964821936	39.0	35.0	41.0	30.0	41.0
31	36.57823698362437	39.0	35.0	40.0	30.0	41.0
32	36.42053324767522	38.0	35.0	40.0	30.0	41.0
33	36.244997541435005	38.0	35.0	40.0	30.0	41.0
34	36.041053659599115	38.0	35.0	40.0	30.0	41.0
35	35.82782265788865	38.0	35.0	40.0	29.0	41.0
36	35.74984190308813	38.0	35.0	40.0	28.0	41.0
37	35.58784110766051	38.0	35.0	40.0	27.0	41.0
38	35.44499118838637	38.0	35.0	40.0	26.0	41.0
39	35.28986821637835	38.0	34.0	40.0	25.0	41.0
40	35.09130108815406	38.0	34.0	40.0	24.0	41.0
41	34.94244332416139	38.0	34.0	40.0	24.0	41.0
42	34.62612892215668	38.0	33.0	40.0	22.0	41.0
43	34.23235108502615	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	1.0
14	5.0
15	8.0
16	30.0
17	60.0
18	189.0
19	422.0
20	908.0
21	1926.0
22	3520.0
23	6157.0
24	10215.0
25	16125.0
26	23912.0
27	33745.0
28	45964.0
29	61905.0
30	77017.0
31	95430.0
32	117200.0
33	145190.0
34	180981.0
35	225878.0
36	300706.0
37	449924.0
38	580503.0
39	707206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77709680587514	20.94081519338064	14.819976966957341	22.462111033786876
2	18.24543706867743	23.994686776874772	36.000846640971396	21.759029513476406
3	19.01924487534249	24.388186830195004	34.122742844473805	22.469825449988704
4	12.827226573376253	16.041480221436156	36.703701266687695	34.427591938499894
5	11.873815488667748	39.074782189292975	35.11203568857485	13.939366633464426
6	31.525026635804682	37.98691985078115	13.336507696750838	17.151545816663333
7	25.294857628504186	34.33984855758244	20.951544066333607	19.413749747579764
8	27.548407151328647	34.16964519844039	18.75108787854527	19.530859771685694
9	24.414863151766877	14.422879013643819	22.163096372182334	38.99916146240697
10	16.320366091186344	28.15019644209828	32.81054657935331	22.71889088736207
11	32.815441019884716	22.908931316737398	23.738944155730078	20.53668350764781
12	21.816790394449768	27.851635569682088	30.64360596447544	19.68796807139271
13	32.407970742266215	21.112898536302975	26.24775009699744	20.23138062443337
14	20.335816964779152	23.149245105478435	28.74112720586114	27.77381072388127
15	22.152724047877353	30.37259657849435	25.844493576127835	21.630185797500463
16	21.18735237670467	28.676624314089537	26.77748414736091	23.358539161844885
17	21.43385714651524	27.069594730599945	27.110403344037355	24.38614477884746
18	23.047466673235807	25.316898817652273	30.392595716294892	21.243038792817035
19	22.61837658214411	24.493368681350205	31.958524964725992	20.92972977177969
20	23.591495083032076	24.298368984416555	31.31725601457052	20.792879917980844
21	22.267792020635742	25.06144791812865	32.24418525878787	20.42657480244774
22	20.65849349120443	25.7019713574747	31.627680232910542	22.011854918410325
23	21.922717756414393	25.85201351124895	32.4061879990263	19.81908073331036
24	21.483255341017713	25.815029692399	30.208260065287302	22.49345490129599
25	21.49502144640118	26.04675390007497	31.5268093790446	20.931415274479253
26	20.90020106102395	27.238047760015533	31.320497365915827	20.541253813044687
27	19.686444636260415	27.211241784389845	31.09250071228696	22.00981286706278
28	20.479214348295518	26.679044307003945	30.96534249901058	21.87639884568996
29	20.641703291235743	26.668250607024074	30.100809268190382	22.5892368335498
30	20.728539093776508	27.048169398207467	32.1033485428343	20.11994296518173
31	21.810761480947498	26.772135917641155	31.72258700030112	19.694515601110226
32	21.106059284964378	26.731975574472806	30.430000910819725	21.731964229743095
33	20.64231914799135	26.547769577519027	31.29946099568479	21.510450278804836
34	19.783166560404364	27.28138462750228	32.02591265919492	20.909536152898433
35	19.189480647997996	29.76435699854755	30.63663705908303	20.409525294371424
36	21.83960950792073	27.13970516019893	30.366178702830638	20.654506629049703
37	20.791453723388912	28.174538990701535	30.301773051599394	20.732234234310155
38	20.434678180811005	26.428098885850304	31.770559000211662	21.366663933127032
39	20.009218403226054	26.11307194859995	32.5531508690225	21.3245587791515
40	18.65109218954256	26.56537011532404	32.44041666923273	22.34312102590067
41	20.166845319148315	25.59925293334193	32.51571326098421	21.71818848652554
42	19.01905039426177	27.60098187014952	31.7799913326265	21.599976402962206
43	19.034219918557806	27.62850094307117	30.988842296264053	22.34843684210697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	859.0
1	958.0
2	1057.0
3	2302.5
4	3548.0
5	3548.0
6	5297.5
7	7047.0
8	7492.0
9	7937.0
10	11930.5
11	15924.0
12	15924.0
13	28505.0
14	41086.0
15	72068.0
16	103050.0
17	94096.0
18	85142.0
19	85142.0
20	85288.0
21	85434.0
22	66217.5
23	47001.0
24	51914.0
25	56827.0
26	56827.0
27	62101.5
28	67376.0
29	71522.5
30	75669.0
31	81917.0
32	88165.0
33	88165.0
34	98154.5
35	108144.0
36	118033.0
37	127922.0
38	136100.5
39	144279.0
40	144279.0
41	150427.0
42	156575.0
43	165300.5
44	174026.0
45	187939.0
46	201852.0
47	201852.0
48	274259.0
49	346666.0
50	320194.0
51	293722.0
52	262709.0
53	231696.0
54	231696.0
55	203484.5
56	175273.0
57	148180.5
58	121088.0
59	107331.5
60	93575.0
61	93575.0
62	82379.0
63	71183.0
64	61146.0
65	51109.0
66	43304.5
67	35500.0
68	35500.0
69	30166.5
70	24833.0
71	20724.5
72	16616.0
73	13595.0
74	10574.0
75	10574.0
76	8463.0
77	6352.0
78	5035.5
79	3719.0
80	2907.5
81	2096.0
82	2096.0
83	1594.5
84	1093.0
85	838.0
86	583.0
87	449.5
88	316.0
89	316.0
90	227.0
91	138.0
92	95.0
93	52.0
94	36.5
95	21.0
96	21.0
97	13.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3085133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.98256073505589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46604586614649	33.63172506477357
2	12.619165014887674	11.100463833859793
3	4.321669600148271	5.7023428699609795
4	1.9680135822177487	3.462331076292282
5	1.1123528936425535	2.446206435172439
6	0.6935725526748938	1.8303058153314762
7	0.4755410113744606	1.464085799035098
8	0.3175219620671347	1.117234318506549
9	0.25677164907623556	1.016412718548239
>10	1.5332770000300908	12.765405480295087
>50	0.1450213286477716	4.425500154702051
>100	0.07920435013882922	6.551474707605864
>500	0.005403454957491345	1.6529587343562788
>1k	0.005329435026550623	4.893276142991686
>5k	6.661793783188279E-4	2.1828379292064723
>10k+	4.441195855458853E-4	5.757438919362051
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	56134	1.8195001641744455	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41241	1.3367657083179234	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40898	1.3256478732035215	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16079	0.5211768828118593	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11376	0.3687361290420867	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11120	0.3604382695981016	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9733	0.3154807264386981	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9344	0.30287186970545515	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9296	0.30131602105970795	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8470	0.27454245894747487	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7925	0.25687709411555354	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6289	0.20384858610633644	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5784	0.18747976181253773	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5124	0.16608684293351372	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5084	0.16479030239539105	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4286	0.13892431865984384	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3845	0.12462995922704143	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3747	0.12145343490864088	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3704	0.12005965383015903	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3688	0.11954103761490996	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3638	0.11792036194225662	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3616	0.11720726464628917	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3504	0.11357695113954568	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3447	0.11172938087272088	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3318	0.10754803763727529	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3274	0.10612184304534035	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3154	0.10223222143097234	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3141	0.10181084575608247	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3121	0.10116257548702114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6206756726533345E-4	0.0
10	3.241351345306669E-5	0.0	0.0	1.9448108071840015E-4	0.0
11	3.241351345306669E-5	0.0	0.0	2.917216210776002E-4	0.0
12	3.241351345306669E-5	0.0	0.0	3.565486479837336E-4	0.0
13	3.241351345306669E-5	0.0	0.0	3.565486479837336E-4	0.0
14	3.241351345306669E-5	0.0	0.0	4.8620270179600036E-4	0.0
15	3.241351345306669E-5	0.0	0.0	6.158567556082672E-4	0.0
16	6.482702690613338E-5	0.0	0.0	9.724054035920007E-4	0.0
17	6.482702690613338E-5	0.0	0.0	0.0015234351322941345	0.0
18	6.482702690613338E-5	0.0	0.0	0.0018475702668248014	0.0
19	6.482702690613338E-5	0.0	0.0	0.002625494589698402	0.0
20	6.482702690613338E-5	0.0	0.0	0.004991681071772271	0.0
21	6.482702690613338E-5	0.0	0.0	0.011020594574042674	0.0
22	6.482702690613338E-5	0.0	0.0	0.016790199968688545	0.0
23	6.482702690613338E-5	0.0	0.0	0.020809475636868816	0.0
24	6.482702690613338E-5	0.0	0.0	0.026708735085326955	0.0
25	6.482702690613338E-5	0.0	0.0	0.03027422156516429	0.0
26	6.482702690613338E-5	0.0	0.0	0.03604382695981016	0.0
27	6.482702690613338E-5	0.0	0.0	0.08164964038827499	0.0
28	6.482702690613338E-5	0.0	0.0	0.19438384017804095	0.0
29	6.482702690613338E-5	0.0	0.0	0.37288505876407924	0.0
30	6.482702690613338E-5	0.0	0.0	0.6236035853235501	0.0
31	6.482702690613338E-5	0.0	0.0	0.9208679172016246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13335	0.0	23.209972	1
CCGTTTA	245	0.0	21.89796	27
GTATTGG	1305	0.0	21.264368	1
ACCGTTA	240	0.0	20.812498	8
ATTGGAC	1420	0.0	19.411972	3
GACCGTT	250	0.0	19.240002	7
TTGGACC	1895	0.0	19.134565	4
TGTTACG	140	9.458745E-11	18.5	16
GCCGTTT	280	0.0	18.5	26
GACCCTC	1925	0.0	17.77922	7
GGACCCT	1970	0.0	17.654823	6
CCGTTAT	280	0.0	17.178572	9
TGGACCC	2020	0.0	17.126236	5
GTTACGG	300	0.0	16.65	17
GGACCGT	345	0.0	16.623188	6
TATTGGA	1640	0.0	16.469511	2
ACGTTTA	225	0.0	16.444445	26
CGTTTAC	305	0.0	16.37705	28
TATACCG	215	0.0	16.348837	5
CAATGCG	175	1.3278623E-10	15.857143	19
>>END_MODULE