FastQCFastQC Report
Wed 25 May 2016
SRR2088527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3120183
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT489601.5691387332089177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT358621.1493556627928554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT346341.1099989968537103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140170.44923647106595993No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG84690.2714263874907337No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA79460.25466455012414335No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA70390.22559574230101245No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG70390.22559574230101245No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA63330.20296886432622702No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61910.19841784920948546No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59550.19085419028306994No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC46270.14829258412086727No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.13858161524500326No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39260.1258259531572347No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT36250.11617908308583183No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA34030.10906411579064434No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT32540.10428875485828876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123400.021.6932741
GTATTAG5350.018.3271031
ATTGGAC9350.018.0053483
GTATTGG9100.017.890111
TTAACGG2100.016.73809435
TTGGACC13950.016.0465954
GGACCCT13000.015.9384616
TGGACCC14300.015.2657345
TATACAC15400.015.13636537
ATTAGAG4550.015.0439563
TAGACCG750.004106504414.85
TATACAG4150.014.7108435
TAACGGC2950.014.42372936
ACCGTTA1803.3378456E-914.3888888
TCTACGC1304.4495555E-614.2307693
CGTATAC1702.4412657E-814.1470593
TAGGGGT2750.014.1272724
GACCCTC14200.013.9401427
GATCGCA2009.858923E-1013.87500110
GTATCAA197850.013.5021482
Produced by FastQC (version 0.11.5)