##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3120183
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78489050161481	31.0	31.0	34.0	30.0	34.0
2	31.94895844250161	31.0	31.0	34.0	30.0	34.0
3	32.067924541605414	33.0	31.0	34.0	30.0	34.0
4	35.732805095085766	37.0	35.0	37.0	35.0	37.0
5	35.648145958105665	37.0	35.0	37.0	33.0	37.0
6	35.697199170689665	37.0	35.0	37.0	33.0	37.0
7	35.55261085647861	37.0	35.0	37.0	33.0	37.0
8	35.585639047453306	37.0	35.0	37.0	33.0	37.0
9	37.205742740089285	39.0	37.0	39.0	33.0	39.0
10	37.06374658153064	39.0	37.0	39.0	33.0	39.0
11	37.13257619825504	39.0	37.0	39.0	33.0	39.0
12	36.9839852341994	39.0	37.0	39.0	33.0	39.0
13	37.03394095795022	39.0	37.0	39.0	33.0	39.0
14	38.07186629758575	40.0	37.0	41.0	33.0	41.0
15	38.07509014695612	40.0	37.0	41.0	33.0	41.0
16	37.97915186384901	40.0	37.0	41.0	33.0	41.0
17	38.0129127041587	40.0	37.0	41.0	33.0	41.0
18	37.981164886803114	40.0	37.0	41.0	33.0	41.0
19	38.0472901749673	40.0	37.0	41.0	33.0	41.0
20	37.946291611742005	40.0	37.0	41.0	33.0	41.0
21	37.81728956282372	40.0	37.0	41.0	32.0	41.0
22	37.69675592745682	40.0	37.0	41.0	32.0	41.0
23	37.52796935307961	39.0	36.0	41.0	32.0	41.0
24	37.67840924714993	40.0	37.0	41.0	32.0	41.0
25	37.63399262158662	40.0	37.0	41.0	32.0	41.0
26	37.4391303330606	39.0	36.0	41.0	32.0	41.0
27	37.39290323676528	39.0	36.0	41.0	32.0	41.0
28	37.245801287937276	39.0	36.0	41.0	31.0	41.0
29	37.02859031024783	39.0	36.0	41.0	31.0	41.0
30	36.878909987010374	39.0	35.0	41.0	31.0	41.0
31	36.6449624909821	39.0	35.0	40.0	30.0	41.0
32	36.49177371968247	39.0	35.0	40.0	30.0	41.0
33	36.32521457876028	38.0	35.0	40.0	30.0	41.0
34	36.134316160302134	38.0	35.0	40.0	30.0	41.0
35	35.937489563913395	38.0	35.0	40.0	29.0	41.0
36	35.869409262213146	38.0	35.0	40.0	28.0	41.0
37	35.72339667256696	38.0	35.0	40.0	27.0	41.0
38	35.58733670428946	38.0	35.0	40.0	27.0	41.0
39	35.44513414758045	38.0	35.0	40.0	26.0	41.0
40	35.25981008165226	38.0	34.0	40.0	25.0	41.0
41	35.1305827254363	38.0	34.0	40.0	24.0	41.0
42	34.84281082231395	38.0	34.0	40.0	23.0	41.0
43	34.45515695714001	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	5.0
14	5.0
15	16.0
16	43.0
17	91.0
18	216.0
19	439.0
20	937.0
21	2004.0
22	3673.0
23	6257.0
24	10012.0
25	15538.0
26	22961.0
27	32939.0
28	45213.0
29	59572.0
30	75627.0
31	93575.0
32	116162.0
33	144378.0
34	182277.0
35	228220.0
36	303115.0
37	448161.0
38	594906.0
39	733835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91167633436885	20.658660085001422	14.38117571950107	23.04848786112866
2	18.526445404003546	23.618037788168195	35.3634386188246	22.49207818900366
3	19.182176173641096	24.01557857343624	33.599599767064944	23.20264548585772
4	13.283419594299437	16.202575297666836	36.23672073080329	34.27728437723044
5	12.470422407916459	38.58462788881293	34.90926653981513	14.035683163455476
6	32.1837853741271	37.72592825484916	13.676281166841816	16.414005204181933
7	25.872713235089094	33.842502186570464	20.96742402609078	19.31736055224966
8	27.699304816416216	34.04290709871825	18.80110878111957	19.456679303745965
9	24.864310843306306	14.44815897016297	21.506270625793423	39.1812595607373
10	16.518870848280372	28.12982443657952	32.6495272873418	22.701777427798305
11	33.70962536492251	22.727577196593916	23.08265252390645	20.480144914577124
12	21.736994272451327	27.612162491751285	30.34248311717614	20.308360118621245
13	32.12798736484366	20.98591653117782	25.722209242214316	21.163886861764198
14	20.651929710533004	22.822475476598648	28.14533634725912	28.380258465609227
15	22.844653662942203	30.17925551161583	24.964240879461236	22.01184994598073
16	21.84423157231483	28.313275214947332	26.223878535329497	23.618614677408345
17	21.861378002508186	27.187059220564947	26.867590779130584	24.083971997796283
18	23.069993010025375	25.54824508690676	29.545831125930754	21.83593077713711
19	23.02464310586911	24.97331086029249	30.827967462164878	21.174078571673522
20	23.663163346508842	24.823031213233328	30.190440752994295	21.323364687263535
21	22.58704056781285	25.472864892860454	30.914789292807505	21.025305246519192
22	21.444992168728565	26.05533072899891	30.331906814440053	22.16777028783248
23	22.2867697183146	26.06584293293054	30.888572881782896	20.75881446697197
24	21.917977246847382	26.21461625808486	29.21020978577218	22.657196709295576
25	21.972204835421515	26.157760618527824	30.365718933793307	21.504315612257358
26	21.444255032477262	27.226576133515245	30.254090865824217	21.075077968183276
27	20.531488056950504	27.129530543561064	29.96503730710667	22.373944092381763
28	21.13215795355593	26.90380660365113	29.853216942724192	22.110818500068746
29	21.318717523940105	26.909575496052636	29.125503215676773	22.64620376433049
30	21.298654598143763	27.185713145671265	30.781335581919393	20.73429667426558
31	22.13841303538927	26.9963332278908	30.348284058979875	20.516969677740054
32	21.408231504370097	26.904223245880125	29.568490053307773	22.119055196442
33	21.0457527651423	26.709426979122696	30.308703047225116	21.936117208509888
34	20.430211945901892	27.121229748383346	30.95914566549462	21.48941264022014
35	19.990558246102875	29.131816947916196	29.87260683107369	21.005017974907243
36	22.08796727627835	27.077257968523	29.580284233328623	21.254490521870032
37	21.06687332121225	27.792632675711648	29.632204264942153	21.50828973813395
38	20.777563367276855	26.36415876889272	30.906392349423093	21.95188551440733
39	20.388099031370917	26.171509812084743	31.630003752985004	21.810387403559343
40	19.34585247083264	26.492228180206094	31.668719430879534	22.49319991808173
41	20.364991412362674	25.499754341331904	31.862329869754436	22.27292437655099
42	19.402611962182988	27.17882252419169	31.17903661419859	22.239528899426734
43	19.34364106207873	26.890057410094215	30.855786343301016	22.910515184526037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	854.0
1	846.0
2	838.0
3	2043.5
4	3249.0
5	3249.0
6	4775.5
7	6302.0
8	6780.0
9	7258.0
10	10757.5
11	14257.0
12	14257.0
13	25741.5
14	37226.0
15	64252.0
16	91278.0
17	83590.0
18	75902.0
19	75902.0
20	76521.5
21	77141.0
22	61111.0
23	45081.0
24	50250.5
25	55420.0
26	55420.0
27	61105.5
28	66791.0
29	71987.0
30	77183.0
31	84598.0
32	92013.0
33	92013.0
34	102746.5
35	113480.0
36	123400.5
37	133321.0
38	142434.5
39	151548.0
40	151548.0
41	156756.5
42	161965.0
43	170881.5
44	179798.0
45	191481.0
46	203164.0
47	203164.0
48	259138.5
49	315113.0
50	296462.5
51	277812.0
52	251889.5
53	225967.0
54	225967.0
55	203261.0
56	180555.0
57	158228.0
58	135901.0
59	122596.0
60	109291.0
61	109291.0
62	97192.5
63	85094.0
64	74202.0
65	63310.0
66	53854.0
67	44398.0
68	44398.0
69	38002.5
70	31607.0
71	27023.5
72	22440.0
73	18478.5
74	14517.0
75	14517.0
76	11661.0
77	8805.0
78	7000.5
79	5196.0
80	4096.0
81	2996.0
82	2996.0
83	2249.0
84	1502.0
85	1132.5
86	763.0
87	601.0
88	439.0
89	439.0
90	345.0
91	251.0
92	165.5
93	80.0
94	66.5
95	53.0
96	53.0
97	36.5
98	20.0
99	12.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3120183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.17021411885826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70019339209153	35.41264351870703
2	12.488123569146829	11.531586782605391
3	4.372999483439792	6.057069674762153
4	2.0311403584723275	3.7511274102448757
5	1.1042099650719883	2.5490805259775353
6	0.6566342802862218	1.819016719115835
7	0.4382727253249891	1.416460189949721
8	0.3095555644613575	1.1433797354291926
9	0.2392040089102734	0.9939690278528942
>10	1.4349527426796131	12.479762054365102
>50	0.1322917971994729	4.252435328815152
>100	0.08168777436881826	7.072318704505418
>500	0.005715686758726615	1.7801935533953446
>1k	0.004251913320418392	3.869465472598673
>5k	4.879244793922745E-4	1.576029335008668
>10k+	2.7881398822415685E-4	4.2954619666669105
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48960	1.5691387332089177	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35862	1.1493556627928554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34634	1.1099989968537103	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14017	0.44923647106595993	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8469	0.2714263874907337	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7946	0.25466455012414335	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7039	0.22559574230101245	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7039	0.22559574230101245	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6333	0.20296886432622702	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6191	0.19841784920948546	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5955	0.19085419028306994	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4627	0.14829258412086727	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4324	0.13858161524500326	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3926	0.1258259531572347	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3625	0.11617908308583183	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3403	0.10906411579064434	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3254	0.10428875485828876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.40988044611486E-5
2	0.0	0.0	0.0	0.0	6.40988044611486E-5
3	0.0	0.0	0.0	0.0	6.40988044611486E-5
4	0.0	0.0	0.0	0.0	6.40988044611486E-5
5	0.0	0.0	0.0	0.0	6.40988044611486E-5
6	0.0	0.0	0.0	0.0	1.6024701115287149E-4
7	0.0	0.0	0.0	0.0	1.6024701115287149E-4
8	6.40988044611486E-5	0.0	0.0	0.0	1.6024701115287149E-4
9	6.40988044611486E-5	0.0	0.0	0.0	1.6024701115287149E-4
10	1.281976089222972E-4	0.0	0.0	3.20494022305743E-5	1.6024701115287149E-4
11	1.281976089222972E-4	0.0	0.0	6.40988044611486E-5	1.6024701115287149E-4
12	1.281976089222972E-4	0.0	0.0	2.2434581561402007E-4	1.6024701115287149E-4
13	1.281976089222972E-4	0.0	0.0	2.884446200751687E-4	2.563952178445944E-4
14	1.6024701115287149E-4	0.0	0.0	4.1664222899746585E-4	2.563952178445944E-4
15	1.9229641338344578E-4	0.0	0.0	5.127904356891888E-4	3.2049402230574297E-4
16	1.9229641338344578E-4	0.0	0.0	6.089386423809117E-4	3.2049402230574297E-4
17	1.9229641338344578E-4	0.0	0.0	8.012350557643574E-4	3.2049402230574297E-4
18	1.9229641338344578E-4	0.0	0.0	0.0010576302736089518	3.2049402230574297E-4
19	2.2434581561402007E-4	0.0	0.0	0.0016665689159898634	3.5254342453631726E-4
20	2.2434581561402007E-4	0.0	0.0	0.003140841418596281	3.5254342453631726E-4
21	2.2434581561402007E-4	0.0	0.0	0.005993238217117393	3.5254342453631726E-4
22	2.563952178445944E-4	0.0	0.0	0.010480154529397795	3.5254342453631726E-4
23	2.563952178445944E-4	0.0	0.0	0.012851810294460293	3.5254342453631726E-4
24	2.563952178445944E-4	0.0	0.0	0.01679388676882093	3.5254342453631726E-4
25	3.2049402230574297E-4	0.0	0.0	0.02012702460080066	3.5254342453631726E-4
26	3.2049402230574297E-4	0.0	0.0	0.0261843616223792	3.5254342453631726E-4
27	3.2049402230574297E-4	0.0	0.0	0.06887416539350416	4.1664222899746585E-4
28	3.2049402230574297E-4	0.0	0.0	0.180309936949211	4.1664222899746585E-4
29	3.2049402230574297E-4	0.0	0.0	0.3577354276976703	4.1664222899746585E-4
30	3.5254342453631726E-4	0.0	0.0	0.5940997691481558	4.1664222899746585E-4
31	3.5254342453631726E-4	0.0	0.0	0.9020624751817441	4.1664222899746585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12340	0.0	21.693274	1
GTATTAG	535	0.0	18.327103	1
ATTGGAC	935	0.0	18.005348	3
GTATTGG	910	0.0	17.89011	1
TTAACGG	210	0.0	16.738094	35
TTGGACC	1395	0.0	16.046595	4
GGACCCT	1300	0.0	15.938461	6
TGGACCC	1430	0.0	15.265734	5
TATACAC	1540	0.0	15.136365	37
ATTAGAG	455	0.0	15.043956	3
TAGACCG	75	0.0041065044	14.8	5
TATACAG	415	0.0	14.710843	5
TAACGGC	295	0.0	14.423729	36
ACCGTTA	180	3.3378456E-9	14.388888	8
TCTACGC	130	4.4495555E-6	14.230769	3
CGTATAC	170	2.4412657E-8	14.147059	3
TAGGGGT	275	0.0	14.127272	4
GACCCTC	1420	0.0	13.940142	7
GATCGCA	200	9.858923E-10	13.875001	10
GTATCAA	19785	0.0	13.502148	2
>>END_MODULE