FastQCFastQC Report
Wed 25 May 2016
SRR2088526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214222
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT372251.6811774067821565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT266891.2053443602312686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261311.1801436351007262No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111580.50392417743117No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG67890.30660882242160004No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57250.25855582683217854No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA56040.253091153461577No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG53190.24021981535726772No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52610.23760038514656617No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47300.2136190499416951No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45650.2061672226181476No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC37270.1683209723324942No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.14822361985383578No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28000.12645525155110915No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27480.1241067968794457No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT26620.12022281415323306No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT24240.10947411777138878No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.1088870041034729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96600.021.027951
GTATTGG8400.020.0416661
TTGGACC12250.016.9142884
CGACGAA3100.016.70967910
TGGACCC12850.016.412455
GTACCGT803.384335E-416.18756
ATTGGAC9200.015.885873
ACGTTTA1403.477544E-815.85714226
GGACCCT11850.015.7679346
TATTGGA10500.015.152382
CCGTTAG750.00410600314.81
CGAACGT2151.2732926E-1114.62790712
GATACTG9650.014.37823936
TTATACT4450.014.1348324
GACCCTC13900.014.1079147
CGGGATT1051.6567249E-414.09523817
TATACTT4250.013.9294125
AAGACCG1203.3030745E-513.8749995
ACCCTCG14150.013.8586578
TACCGTG1356.573073E-613.7037047
Produced by FastQC (version 0.11.5)