##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088526_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2214222
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77481255267087	31.0	31.0	34.0	30.0	34.0
2	31.935116713680923	31.0	31.0	34.0	30.0	34.0
3	32.05356915431244	33.0	31.0	34.0	30.0	34.0
4	35.70901427228164	37.0	35.0	37.0	35.0	37.0
5	35.62934610892675	37.0	35.0	37.0	33.0	37.0
6	35.688587232897156	37.0	35.0	37.0	33.0	37.0
7	35.537871089710066	37.0	35.0	37.0	33.0	37.0
8	35.58176551402705	37.0	35.0	37.0	33.0	37.0
9	37.19253805625633	39.0	37.0	39.0	33.0	39.0
10	37.057407974448815	39.0	37.0	39.0	33.0	39.0
11	37.122080351473336	39.0	37.0	39.0	33.0	39.0
12	36.97928437166644	39.0	37.0	39.0	33.0	39.0
13	37.02837971982936	39.0	37.0	39.0	33.0	39.0
14	38.05861381559753	40.0	37.0	41.0	33.0	41.0
15	38.06542297926766	40.0	37.0	41.0	33.0	41.0
16	37.9650391875792	40.0	37.0	41.0	33.0	41.0
17	38.012056153357705	40.0	37.0	41.0	33.0	41.0
18	37.989094137805516	40.0	37.0	41.0	33.0	41.0
19	38.05395077819659	40.0	37.0	41.0	33.0	41.0
20	37.95878868514539	40.0	37.0	41.0	33.0	41.0
21	37.826458232282036	40.0	37.0	41.0	32.0	41.0
22	37.714948184960676	40.0	37.0	41.0	32.0	41.0
23	37.55021086413196	39.0	36.0	41.0	32.0	41.0
24	37.70186774406541	40.0	37.0	41.0	32.0	41.0
25	37.658754180926756	40.0	37.0	41.0	32.0	41.0
26	37.45901043346151	39.0	36.0	41.0	32.0	41.0
27	37.4111782829364	39.0	36.0	41.0	32.0	41.0
28	37.2627753676009	39.0	36.0	41.0	31.0	41.0
29	37.04547692146497	39.0	36.0	41.0	31.0	41.0
30	36.894655097817655	39.0	35.0	41.0	31.0	41.0
31	36.6509667955607	39.0	35.0	40.0	30.0	41.0
32	36.50402263187702	39.0	35.0	40.0	30.0	41.0
33	36.33409341972033	38.0	35.0	40.0	30.0	41.0
34	36.1387114751818	38.0	35.0	40.0	30.0	41.0
35	35.947671462030456	38.0	35.0	40.0	29.0	41.0
36	35.866801522159925	38.0	35.0	40.0	28.0	41.0
37	35.71684907836703	38.0	35.0	40.0	27.0	41.0
38	35.57782507806353	38.0	35.0	40.0	27.0	41.0
39	35.422372734079964	38.0	35.0	40.0	26.0	41.0
40	35.234225836433744	38.0	34.0	40.0	25.0	41.0
41	35.08887320241602	38.0	34.0	40.0	24.0	41.0
42	34.79774205115837	38.0	34.0	40.0	23.0	41.0
43	34.403670002375556	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	6.0
15	12.0
16	24.0
17	56.0
18	137.0
19	303.0
20	648.0
21	1319.0
22	2538.0
23	4373.0
24	7108.0
25	11016.0
26	16687.0
27	23403.0
28	32249.0
29	42350.0
30	54270.0
31	67178.0
32	82836.0
33	103405.0
34	129088.0
35	161220.0
36	214382.0
37	316569.0
38	416067.0
39	526975.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.899141097866426	20.95015766260113	14.524966331289274	22.625734908243167
2	18.365141345357422	24.06904095433972	35.46302041981337	22.10279728048949
3	19.3360467017309	24.276608217242895	33.59514086663397	22.792204214392235
4	13.219225533844392	16.167168423039787	36.39747956618623	34.216126476929595
5	12.146162399253553	39.094002317744106	34.897178331712	13.862656951290338
6	31.895130659888665	37.96986029404459	13.522627812387375	16.61238123367937
7	25.496991719890776	34.13040788141387	21.106826686754985	19.265773711940355
8	27.687016026396634	34.29014796167683	18.853981217782138	19.168854794144398
9	24.670155025105885	14.421363350197044	21.916185459271926	38.992296165425145
10	16.28892676524757	28.437256968813422	32.79070481640956	22.48311144952945
11	33.06326104609204	22.94232466301934	23.392098895232728	20.6023153956559
12	21.435158714889475	27.996786230107006	30.62538444654601	19.942670608457508
13	32.11362726953305	21.19534536284076	25.940669002475815	20.75035836515038
14	20.291190314250333	23.308096478130917	28.582906321046398	27.81780688657235
15	22.3454558756981	30.41695909443588	25.521650493943245	21.715934535922777
16	21.299128994292353	28.7389430689425	26.618830451508472	23.343097485256674
17	21.359646864677526	27.38772354352906	27.299701655931518	23.952927935861894
18	22.86753541424482	25.733146902162474	29.94415194140425	21.455165742188452
19	22.557720047944603	25.18600212625473	31.430226960078983	20.826050865721683
20	23.407634826137578	25.065056710664063	30.7436201067463	20.783688356452064
21	22.134275605607748	25.727862879151232	31.56214688500069	20.575714630240327
22	20.811463349203468	26.280111027710863	31.038306005450224	21.87011961763545
23	21.81055016163691	26.277039971601763	31.616883943886386	20.29552592287494
24	21.537226167927155	26.37093299587846	29.848407250944124	22.243433585250262
25	21.500192844258613	26.542325024320057	30.957058506328632	21.000423625092697
26	20.921118117334213	27.63024665096815	30.837919594331552	20.61071563736608
27	20.02635688743044	27.50243652172185	30.59309319481064	21.878113396037076
28	20.628916161071473	27.112999509534276	30.541382029444204	21.71670229995005
29	20.71138305011873	27.120044873549265	29.793263728749874	22.37530834758213
30	20.888555890059806	27.47235823688862	31.41871049966986	20.22037537338171
31	21.646519635339185	27.27124922433252	31.110701636963235	19.971529503365065
32	21.047302393346285	27.143348769906538	30.231250525015106	21.578098311732067
33	20.648290912112696	26.888812413570097	31.01491178391327	21.447984890403944
34	19.754387771415875	27.557309068377062	31.595612364071897	21.092690796135166
35	19.492760888474596	29.41294955970991	30.485651393582035	20.608638158233454
36	21.599415054136397	27.272423451668352	30.219011463168556	20.909150031026698
37	20.666807573946965	27.970998391308548	30.296194329204567	21.065999705539916
38	20.317429778947186	26.514956494877207	31.664259500628212	21.503354225547394
39	19.793950200115436	26.35395186209874	32.358860132362516	21.493237805423306
40	18.84643906527891	26.57655826741853	32.25056024192696	22.326442425375596
41	19.846745267638024	25.810103955249293	32.42561044014557	21.917540336967114
42	19.078529614465037	27.406736993851567	31.642536294915324	21.872197096768076
43	18.9274607514513	27.298617753775368	31.2745515129016	22.499369981871737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	717.0
1	698.0
2	679.0
3	1535.0
4	2391.0
5	2391.0
6	3728.5
7	5066.0
8	5419.5
9	5773.0
10	8717.0
11	11661.0
12	11661.0
13	20837.0
14	30013.0
15	50509.5
16	71006.0
17	64737.5
18	58469.0
19	58469.0
20	59251.5
21	60034.0
22	48022.0
23	36010.0
24	39991.5
25	43973.0
26	43973.0
27	48113.5
28	52254.0
29	55688.5
30	59123.0
31	63509.5
32	67896.0
33	67896.0
34	74841.0
35	81786.0
36	88397.0
37	95008.0
38	99583.5
39	104159.0
40	104159.0
41	108093.0
42	112027.0
43	117580.5
44	123134.0
45	132617.5
46	142101.0
47	142101.0
48	184959.0
49	227817.0
50	211255.5
51	194694.0
52	175463.0
53	156232.0
54	156232.0
55	140116.0
56	124000.0
57	107730.0
58	91460.0
59	82668.5
60	73877.0
61	73877.0
62	65237.0
63	56597.0
64	49278.0
65	41959.0
66	35552.0
67	29145.0
68	29145.0
69	24821.0
70	20497.0
71	17048.5
72	13600.0
73	11281.5
74	8963.0
75	8963.0
76	7194.5
77	5426.0
78	4295.0
79	3164.0
80	2447.0
81	1730.0
82	1730.0
83	1297.0
84	864.0
85	667.5
86	471.0
87	357.0
88	243.0
89	243.0
90	187.0
91	131.0
92	85.5
93	40.0
94	30.0
95	20.0
96	20.0
97	15.0
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2214222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.595105688168466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40183682325586	41.48341268418762
2	11.169756312735823	11.526095149333832
3	3.476791647228246	5.38156297483448
4	1.5834085216144593	3.2678452008097847
5	0.8008251338626984	2.065932870969379
6	0.5156755434850571	1.5963800500149141
7	0.33347460306805016	1.2043960172731254
8	0.24623601935155784	1.016365875414202
9	0.18703746457035939	0.8685195976939281
>10	1.114296418527383	10.815383114085426
>50	0.10261425939738858	3.636741259400404
>100	0.05876649866441468	5.703578934341512
>500	0.004377714263501646	1.6306811618553043
>1k	0.004115051407691546	3.9349929261147927
>5k	4.377714263501644E-4	1.2963955893103083
>10k+	3.5021714108013157E-4	4.5717165943609706
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37225	1.6811774067821565	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26689	1.2053443602312686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26131	1.1801436351007262	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11158	0.50392417743117	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6789	0.30660882242160004	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5725	0.25855582683217854	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5604	0.253091153461577	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5319	0.24021981535726772	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5261	0.23760038514656617	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4730	0.2136190499416951	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4565	0.2061672226181476	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3727	0.1683209723324942	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3282	0.14822361985383578	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2800	0.12645525155110915	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2748	0.1241067968794457	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2662	0.12022281415323306	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2424	0.10947411777138878	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2411	0.1088870041034729	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.8065035935872735E-4	0.0	0.0	0.0	0.0
7	1.8065035935872735E-4	0.0	0.0	0.0	0.0
8	1.8065035935872735E-4	0.0	0.0	4.5162589839681836E-5	0.0
9	1.8065035935872735E-4	0.0	0.0	4.5162589839681836E-5	0.0
10	1.8065035935872735E-4	0.0	0.0	4.5162589839681836E-5	0.0
11	1.8065035935872735E-4	0.0	0.0	3.1613812887777286E-4	0.0
12	1.8065035935872735E-4	0.0	0.0	6.322762577555457E-4	0.0
13	1.8065035935872735E-4	0.0	0.0	7.226014374349094E-4	0.0
14	1.8065035935872735E-4	0.0	0.0	0.0010387395663126822	0.0
15	1.8065035935872735E-4	0.0	0.0	0.0013097151053507734	0.0
16	1.8065035935872735E-4	0.0	0.0	0.001625853234228546	0.0
17	1.8065035935872735E-4	0.0	0.0	0.002258129491984092	0.0
18	1.8065035935872735E-4	0.0	0.0	0.002574267620861865	0.0
19	1.8065035935872735E-4	0.0	0.0	0.0031613812887777287	0.0
20	1.8065035935872735E-4	0.0	0.0	0.006413087757234821	0.0
21	2.258129491984092E-4	0.0	0.0	0.012690687744950597	0.0
22	2.7097553903809103E-4	0.0	0.0	0.020232840248177463	0.0
23	2.7097553903809103E-4	0.0	0.0	0.025471700669580558	0.0
24	2.7097553903809103E-4	0.0	0.0	0.031794463247136015	0.0
25	2.7097553903809103E-4	0.0	0.0	0.03576877115302802	0.0
26	2.7097553903809103E-4	0.0	0.0	0.04412385027336915	0.0
27	2.7097553903809103E-4	0.0	0.0	0.10026094944409368	0.0
28	2.7097553903809103E-4	4.5162589839681836E-5	0.0	0.24338119664604543	0.0
29	2.7097553903809103E-4	4.5162589839681836E-5	0.0	0.45266463796313106	0.0
30	2.7097553903809103E-4	4.5162589839681836E-5	0.0	0.7277499726766331	0.0
31	2.7097553903809103E-4	4.5162589839681836E-5	0.0	1.0810117504026244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9660	0.0	21.02795	1
GTATTGG	840	0.0	20.041666	1
TTGGACC	1225	0.0	16.914288	4
CGACGAA	310	0.0	16.709679	10
TGGACCC	1285	0.0	16.41245	5
GTACCGT	80	3.384335E-4	16.1875	6
ATTGGAC	920	0.0	15.88587	3
ACGTTTA	140	3.477544E-8	15.857142	26
GGACCCT	1185	0.0	15.767934	6
TATTGGA	1050	0.0	15.15238	2
CCGTTAG	75	0.004106003	14.8	1
CGAACGT	215	1.2732926E-11	14.627907	12
GATACTG	965	0.0	14.378239	36
TTATACT	445	0.0	14.134832	4
GACCCTC	1390	0.0	14.107914	7
CGGGATT	105	1.6567249E-4	14.095238	17
TATACTT	425	0.0	13.929412	5
AAGACCG	120	3.3030745E-5	13.874999	5
ACCCTCG	1415	0.0	13.858657	8
TACCGTG	135	6.573073E-6	13.703704	7
>>END_MODULE