FastQCFastQC Report
Wed 25 May 2016
SRR2088525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719621
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT153542.1336231155010763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110061.5294161787941152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109031.5151030889871198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44190.6140732413312008No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32720.45468378493679307No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA28140.391039172008599No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG26410.3669987396143248No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA25580.3554648905465516No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG23990.3333699266697331No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21930.30474374705574186No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA20660.28709556836167927No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16480.22900943691193001No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14140.19649232026302735No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.19301826933899927No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12960.18009479990161487No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11730.1630024693553968No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT11660.16202973509666896No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT10590.14716079714182884No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10020.13923996103504485No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG9550.13270874529787208No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.129234694373844No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9240.12840092215207727No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT8790.12214763048882676No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG8740.12145282030402114No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC8420.11700603512126521No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8000.11116962956889809No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT8000.11116962956889809No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA7920.11005793327320909No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT7800.10839038882967562No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT7770.10797350271879226No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.10088643883377499No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.10046955272289163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.025.0866871
GTATTGG2450.024.9183671
TCACGTA400.00193048923.12500225
CAACGGT502.7007415E-422.24
TGGACCC3450.021.9855065
TAGACAG705.097547E-621.1428595
GGACCCT3500.021.1428596
ATTGGAC2900.021.0517233
TTGGACC3750.020.724
TATTGGA3050.020.0163942
GGACACT759.2578775E-619.7333346
GTATTAG851.243985E-619.5882361
TGAACCG609.232239E-418.55
ATCACGT500.007032387418.524
GCTTCGC500.007032387418.531
GTATATG500.007032387418.51
GTATAAT609.232239E-418.51
GGTTAGT500.007032387418.58
CCGCACG500.007032387418.523
ATACAGG1258.561983E-917.766
Produced by FastQC (version 0.11.5)