FastQCFastQC Report
Wed 25 May 2016
SRR2088524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535108
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270571.7625469999504921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192671.2550908470283524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191111.2449286955706047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.5022447932002179No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG48700.3172415230719923No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41370.2694924396198834No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA39360.2563988983185548No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG38860.25314179849235363No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA34460.22447932002178347No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30740.20024649731484692No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29440.19177803776672392No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.16389726325444204No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC23670.15419110577236259No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT19590.1276131711905612No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19000.12376979339564383No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17780.11582246981971302No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17670.11510590785794876No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.1112625300630314No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15900.10357577447319669No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG15410.10038381664351954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.023.3634821
TGCGACG802.7273927E-823.12522
TATACCG1059.840733E-1021.1428575
GTATTGG5100.020.676471
CAATGCG909.483483E-820.55555519
CGACGAG1001.290573E-820.3524
ACGTTTA759.269097E-619.73333426
TTGGACC7500.018.5000024
CCGATCG801.6172467E-518.512
CGATCGT801.6172467E-518.513
ATTGGAC5450.017.9908263
CCGTCGG953.6079819E-617.52631624
CGACCTA852.72408E-517.41176413
ACGAGTC1005.880376E-616.65000226
TACCGAC3450.016.6231887
TGGACCC7950.016.5220155
GGACCCT7950.016.5220156
CTAGACC904.4475208E-516.4444453
TAGTTCG803.3834908E-416.18757
TATACGG1151.2427827E-616.0869562
Produced by FastQC (version 0.11.5)