FastQCFastQC Report
Wed 25 May 2016
SRR2088523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689565
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT563862.0964728496987433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399291.4845895154049076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397621.4783803328791085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158180.5881248454675756No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG99120.3685354323096858No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA81190.30187037680814555No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA80660.29989979792271243No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG79800.2967022548255945No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA76630.28491596224668303No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG59560.22144844984226075No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58340.21691240033239576No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.18794860878989725No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC49680.1847138849590919No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT44060.16381831262676305No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT40230.14957809162448202No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38860.14448433110930578No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37460.1392790283930673No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.1349288825516394No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG35040.13028129084071216No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG34510.12831071195527902No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT33900.12604268720034653No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA32020.11905270926711198No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30200.11228581573600191No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30130.11202555060019No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT30100.11191400839912775No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29760.11064986345375553No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT27840.10351116258577131No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27150.10094569196133947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129150.022.4607031
GTATTGG8850.020.6949141
ATTGGAC8550.020.3391823
ACGTTTA1551.8189894E-1219.09677526
TTGGACC12800.018.7890624
GGACCCT13500.017.8148166
TATACCG2250.017.2666665
TGGACCC14950.017.0769235
TATACTG2600.017.0769235
TTGCGAT3550.016.67605611
GACCCTC13350.016.6292137
TATTGGA10700.015.9065422
GTATTAG3750.015.7866681
ATTAGAC2003.6379788E-1215.7249993
ATACACA12000.015.41666637
CGCCCTA1101.4532279E-515.13636424
TATACAC17950.014.73816237
CTTGCGA4800.014.64583310
CGGTATA1152.211912E-514.4782626
ACCCTCG15350.014.4625418
Produced by FastQC (version 0.11.5)