##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2689565
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73232511577151	31.0	31.0	34.0	30.0	34.0
2	31.883283728037807	31.0	31.0	34.0	30.0	34.0
3	31.991270707344867	33.0	31.0	34.0	30.0	34.0
4	35.662120826230264	37.0	35.0	37.0	33.0	37.0
5	35.5889428216087	37.0	35.0	37.0	33.0	37.0
6	35.65059256794314	37.0	35.0	37.0	33.0	37.0
7	35.49030121971397	37.0	35.0	37.0	33.0	37.0
8	35.55108279591681	37.0	35.0	37.0	33.0	37.0
9	37.13282519663961	39.0	37.0	39.0	33.0	39.0
10	37.00973317246469	39.0	37.0	39.0	33.0	39.0
11	37.06752987936711	39.0	37.0	39.0	33.0	39.0
12	36.91408387601712	39.0	37.0	39.0	33.0	39.0
13	36.96925673854322	39.0	37.0	39.0	33.0	39.0
14	37.945885672961985	40.0	37.0	41.0	33.0	41.0
15	37.9576578368621	40.0	37.0	41.0	33.0	41.0
16	37.851722118632566	40.0	37.0	41.0	32.0	41.0
17	37.934903599652735	40.0	37.0	41.0	33.0	41.0
18	37.919917161325344	40.0	37.0	41.0	33.0	41.0
19	37.99963116712182	40.0	37.0	41.0	33.0	41.0
20	37.90675480979266	40.0	37.0	41.0	32.0	41.0
21	37.78214469626129	40.0	37.0	41.0	32.0	41.0
22	37.67728907834538	40.0	37.0	41.0	32.0	41.0
23	37.49259378375314	39.0	36.0	41.0	32.0	41.0
24	37.621005255496705	40.0	37.0	41.0	32.0	41.0
25	37.55889037818383	40.0	36.0	41.0	32.0	41.0
26	37.332374937954654	39.0	36.0	41.0	31.0	41.0
27	37.283972687033035	39.0	36.0	41.0	31.0	41.0
28	37.112527862312305	39.0	36.0	41.0	31.0	41.0
29	36.87964113155845	39.0	35.0	41.0	31.0	41.0
30	36.70232323814446	39.0	35.0	41.0	30.0	41.0
31	36.39637339123613	38.0	35.0	40.0	30.0	41.0
32	36.222998142822355	38.0	35.0	40.0	30.0	41.0
33	36.02950105314428	38.0	35.0	40.0	30.0	41.0
34	35.81803934837046	38.0	35.0	40.0	29.0	41.0
35	35.60333994530714	38.0	35.0	40.0	27.0	41.0
36	35.48792537083134	38.0	35.0	40.0	27.0	41.0
37	35.3037736585656	38.0	34.0	40.0	25.0	41.0
38	35.14185267877891	38.0	34.0	40.0	25.0	41.0
39	34.96928388047881	38.0	34.0	40.0	24.0	41.0
40	34.75076266979976	38.0	34.0	40.0	23.0	41.0
41	34.56237086666431	38.0	33.0	40.0	22.0	41.0
42	34.218174686241085	38.0	33.0	40.0	20.0	41.0
43	33.78293664588883	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	10.0
15	9.0
16	36.0
17	84.0
18	174.0
19	435.0
20	921.0
21	1826.0
22	3481.0
23	6023.0
24	9999.0
25	15471.0
26	22776.0
27	32181.0
28	44090.0
29	57614.0
30	71870.0
31	86800.0
32	105524.0
33	130762.0
34	161583.0
35	199168.0
36	264223.0
37	394445.0
38	489109.0
39	590945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.568432069870035	21.190378369736372	15.520167759470397	21.7210218009232
2	18.42978325491297	24.319508916869456	35.70975232054254	21.540955507675033
3	19.427379520480077	24.294932451902074	34.24379035271503	22.033897674902818
4	13.21295451123137	16.282670245931964	35.90576914854261	34.59860609429406
5	11.968478173979808	39.408528888500555	34.414747366209774	14.208245571309858
6	31.551087257604856	38.18256855662533	13.06133147925408	17.20501270651574
7	24.42755612896509	35.052396948948996	20.80592958340847	19.714117338677443
8	27.807805351423	34.11800793065049	18.53816509361179	19.53602162431471
9	24.163907546387613	14.657872183791804	22.450768061006148	38.72745220881444
10	16.487647630750697	28.76915040164488	32.03696508543203	22.706236882172394
11	32.85668872103854	23.087934294207425	23.44542704861195	20.60994993614209
12	21.73355170817586	28.613400308228282	30.41986343516517	19.233184548430692
13	32.701719423029374	21.550845582835887	26.080983356044563	19.666451638090173
14	20.097710968130535	23.890629153785092	29.185463076742895	26.826196801341478
15	21.99158599996654	30.815838248936167	26.168804249014244	21.02377150208305
16	20.730824501359884	28.894672558573596	27.14011373586435	23.234389204202166
17	21.093299474078524	27.2815120660776	27.6313827700762	23.993805689767676
18	22.719473223365117	25.478097759303086	30.941695032468076	20.860733984863725
19	22.26817347786724	25.026389025734648	32.59958394759004	20.105853548808078
20	23.0951101758091	24.520359240248887	31.91296733858449	20.471563245357522
21	21.60319605586777	25.589230972294775	32.93350411683674	19.874068855000715
22	20.186833186779275	26.087452803706174	32.24737085736913	21.47834315214542
23	21.45413849451491	26.06752393044972	32.87553935301805	19.602798222017313
24	20.94647275674691	26.304476746239636	30.89410369334818	21.854946803665275
25	20.895535151595148	26.43297336186335	32.269679297581575	20.401812188959926
26	20.298561291509966	27.800889734957142	31.999784351744616	19.90076462178828
27	19.247461950166663	27.41097538077719	31.723903307783974	21.617659361272175
28	19.973713221282996	27.261880638690645	31.549265401654168	21.21514073837219
29	20.12485290372235	27.033219126513025	30.614021226480865	22.227906743283764
30	20.181665064796725	27.470836362013934	32.92342813800745	19.424070435181896
31	21.314710743187096	27.16201318800624	32.22680991163999	19.29646615716668
32	20.473013293971327	27.143943351434153	31.18876100782097	21.194282346773548
33	19.962373097508333	26.74603513951141	32.16245006162707	21.129141701353195
34	19.1214564437	27.32616612723619	32.95700234052718	20.59537508853662
35	18.555156688906944	29.433384208970597	31.686425128227054	20.325033973895408
36	21.191345068812243	27.063781689604085	31.3887189935919	20.356154247991775
37	20.10916263410626	28.08011704494965	31.22716870572007	20.583551615224025
38	19.59350303859546	26.184197072760835	32.978976154136454	21.243323734507253
39	19.14380206464614	25.996731813508877	33.749807124944	21.109658996900986
40	17.860509041424915	26.236584726526406	33.758990766164786	22.143915465883886
41	19.07312148990636	25.43902080819761	33.91299336509807	21.57486433679796
42	18.238785825960704	27.39037725431436	32.85966317973353	21.51117373999141
43	18.11185080115186	27.427186180664904	32.32660300085702	22.13436001732622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1568.0
1	1262.5
2	957.0
3	2238.5
4	3520.0
5	3520.0
6	5084.0
7	6648.0
8	7203.5
9	7759.0
10	12104.0
11	16449.0
12	16449.0
13	29231.0
14	42013.0
15	73368.5
16	104724.0
17	95481.0
18	86238.0
19	86238.0
20	85932.0
21	85626.0
22	66356.0
23	47086.0
24	50797.0
25	54508.0
26	54508.0
27	57947.0
28	61386.0
29	63287.0
30	65188.0
31	69727.0
32	74266.0
33	74266.0
34	82487.0
35	90708.0
36	99643.5
37	108579.0
38	114244.5
39	119910.0
40	119910.0
41	124363.0
42	128816.0
43	137460.0
44	146104.0
45	161561.0
46	177018.0
47	177018.0
48	240902.5
49	304787.0
50	277516.5
51	250246.0
52	221303.5
53	192361.0
54	192361.0
55	169474.0
56	146587.0
57	123092.5
58	99598.0
59	88003.0
60	76408.0
61	76408.0
62	67117.5
63	57827.0
64	50616.5
65	43406.0
66	37211.0
67	31016.0
68	31016.0
69	26439.0
70	21862.0
71	18249.0
72	14636.0
73	11959.5
74	9283.0
75	9283.0
76	7444.5
77	5606.0
78	4402.0
79	3198.0
80	2495.5
81	1793.0
82	1793.0
83	1359.0
84	925.0
85	691.5
86	458.0
87	353.5
88	249.0
89	249.0
90	201.5
91	154.0
92	104.0
93	54.0
94	40.0
95	26.0
96	26.0
97	17.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2689565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.27703462476157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07220630514944	33.35456540874719
2	12.30332220710296	10.649026023127854
3	4.254583198596552	5.523772331987754
4	1.9164299209291462	3.317496161759189
5	1.0680856384240915	2.311178957814499
6	0.6489417475427673	1.6850564686716973
7	0.43857166807768694	1.3286056885386248
8	0.31155208057079115	1.0786440142622908
9	0.2586920201799997	1.0075881163031428
>10	1.472282619907946	12.043166450295898
>50	0.1442989754910747	4.352183714399516
>100	0.09663391100323011	7.980546856293113
>500	0.007156740616907995	2.0895447894870354
>1k	0.006208256920691089	5.424360654943157
>5k	6.898063245212321E-4	2.186155823489092
>10k+	3.4490316226061605E-4	5.668108539880099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	56386	2.0964728496987433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39929	1.4845895154049076	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39762	1.4783803328791085	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15818	0.5881248454675756	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9912	0.3685354323096858	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8119	0.30187037680814555	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8066	0.29989979792271243	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7980	0.2967022548255945	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7663	0.28491596224668303	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5956	0.22144844984226075	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5834	0.21691240033239576	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5055	0.18794860878989725	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4968	0.1847138849590919	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4406	0.16381831262676305	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4023	0.14957809162448202	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3886	0.14448433110930578	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3746	0.1392790283930673	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3629	0.1349288825516394	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3504	0.13028129084071216	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3451	0.12831071195527902	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3390	0.12604268720034653	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3202	0.11905270926711198	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3020	0.11228581573600191	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3013	0.11202555060019	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3010	0.11191400839912775	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2976	0.11064986345375553	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	2784	0.10351116258577131	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2715	0.10094569196133947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.7180733687417854E-5	0.0	0.0	0.0	0.0
3	3.7180733687417854E-5	0.0	0.0	0.0	0.0
4	3.7180733687417854E-5	0.0	0.0	0.0	0.0
5	3.7180733687417854E-5	0.0	0.0	0.0	0.0
6	3.7180733687417854E-5	0.0	0.0	0.0	0.0
7	3.7180733687417854E-5	0.0	0.0	0.0	0.0
8	3.7180733687417854E-5	0.0	0.0	0.0	0.0
9	3.7180733687417854E-5	0.0	0.0	7.436146737483571E-5	0.0
10	7.436146737483571E-5	0.0	0.0	2.60265135811925E-4	0.0
11	1.1154220106225357E-4	0.0	0.0	6.320724726861035E-4	0.0
12	1.1154220106225357E-4	0.0	0.0	0.0010038798095602821	0.0
13	1.1154220106225357E-4	0.0	0.0	0.0011897834779973713	0.0
14	1.1154220106225357E-4	0.0	0.0	0.0013013256790596248	0.0
15	1.1154220106225357E-4	0.0	0.0	0.001747494483308639	0.0
16	1.1154220106225357E-4	0.0	0.0	0.002565470624431832	0.0
17	1.1154220106225357E-4	0.0	0.0	0.0036808926350543674	0.0
18	1.1154220106225357E-4	0.0	0.0	0.004015519238241129	0.0
19	1.4872293474967141E-4	0.0	0.0	0.004907856846739157	0.0
20	1.4872293474967141E-4	0.0	0.0	0.007919496275420003	0.0
21	1.8590366843708927E-4	0.0	0.0	0.014686389806530052	0.0
22	2.2308440212450714E-4	0.0	0.0	0.022234078745075877	0.0
23	2.2308440212450714E-4	0.0	0.0	0.02773682733081372	0.0
24	2.2308440212450714E-4	0.0	0.0	0.0349870703998602	0.0
25	2.2308440212450714E-4	0.0	0.0	0.039002589638101326	0.0
26	2.2308440212450714E-4	0.0	0.0	0.04729389325039551	0.0
27	2.2308440212450714E-4	0.0	0.0	0.10890236897044689	0.0
28	2.2308440212450714E-4	0.0	0.0	0.2724232357277106	0.0
29	2.2308440212450714E-4	0.0	0.0	0.5193404881458525	0.0
30	2.2308440212450714E-4	0.0	0.0	0.8625558408144068	0.0
31	2.2308440212450714E-4	0.0	0.0	1.3376884366059196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12915	0.0	22.460703	1
GTATTGG	885	0.0	20.694914	1
ATTGGAC	855	0.0	20.339182	3
ACGTTTA	155	1.8189894E-12	19.096775	26
TTGGACC	1280	0.0	18.789062	4
GGACCCT	1350	0.0	17.814816	6
TATACCG	225	0.0	17.266666	5
TGGACCC	1495	0.0	17.076923	5
TATACTG	260	0.0	17.076923	5
TTGCGAT	355	0.0	16.676056	11
GACCCTC	1335	0.0	16.629213	7
TATTGGA	1070	0.0	15.906542	2
GTATTAG	375	0.0	15.786668	1
ATTAGAC	200	3.6379788E-12	15.724999	3
ATACACA	1200	0.0	15.416666	37
CGCCCTA	110	1.4532279E-5	15.136364	24
TATACAC	1795	0.0	14.738162	37
CTTGCGA	480	0.0	14.645833	10
CGGTATA	115	2.211912E-5	14.47826	26
ACCCTCG	1535	0.0	14.462541	8
>>END_MODULE