FastQCFastQC Report
Wed 25 May 2016
SRR2088522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739898
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1198803.2054350145378296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT853422.28193389231471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT853322.281666505343194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330080.88259091558112No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG279270.7467315953536701No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA229070.6125033356524696No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA225770.6036795655924306No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG222920.596059036904215No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA186620.4989975662437853No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG183470.4905748766410207No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA138260.36968922681848543No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC134220.35888679316922545No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA111060.2969599705660422No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT106200.2839649637503483No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT104050.27821614386274707No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA95770.25607650262119447No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT95540.2554615125867069No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA84720.2265302422686394No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG82770.22131619632407085No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG82610.2208883771696447No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA78710.21046028528050767No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA77100.20615535503909466No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT76620.20487189757581623No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT75990.20318735965526333No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA72730.19447054438383077No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT70890.1895506241079302No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67120.17947013528176436No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG66670.17826689390994085No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA62100.16604730931164433No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC61460.1643360326939398No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60920.16289214304775157No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA58470.15634116224560135No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.14890780443744722No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT55310.14789173394568514No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA55110.1473569600026525No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG53150.1421161753609323No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49710.13291806354077035No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG47560.12716924365316915No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA46290.12377342911491168No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.11928132799343726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC44110.11794439313585557No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.11775722225579414No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA42840.11454857859759812No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA40440.10813129128120606No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA40340.10786390430968973No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA40300.1077569495210832No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG39830.10650023075495642No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA38250.10227551660499833No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA37900.10133966220469114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG11000.025.731821
GGTATCA253000.024.861661
ATTGGAC11750.024.4042553
TTGGACC21100.020.1658764
TATTGGA14550.019.5807552
GGACCCT21200.019.3726436
TTTTTAC35200.019.2357961
TGGACCC22250.019.1235965
CCGTTTA902.1548567E-618.527
ACTCTGT25150.017.65407637
GACCCTC23100.017.6190477
CGCCGTT1005.88492E-616.65000225
TACCGAC7750.016.232267
CTCTAAT2650.016.0566041
ACGGTGT1851.8189894E-1116.029
GATACCT25900.015.92857236
AGCCGTC1751.3278623E-1015.85714312
CGTTTAC1059.354289E-615.85714228
GTTTAGA1659.767973E-1015.696971
ACCGACG6450.015.4883728
Produced by FastQC (version 0.11.5)