##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3739898
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643829591074407	31.0	31.0	34.0	30.0	34.0
2	31.78107344104037	31.0	31.0	34.0	30.0	34.0
3	31.851844087726455	31.0	31.0	34.0	30.0	34.0
4	35.535823971669814	37.0	35.0	37.0	33.0	37.0
5	35.51452446029277	37.0	35.0	37.0	33.0	37.0
6	35.61079018732597	37.0	35.0	37.0	33.0	37.0
7	35.40978203148856	37.0	35.0	37.0	33.0	37.0
8	35.5085791109811	37.0	35.0	37.0	33.0	37.0
9	37.04405815345766	39.0	37.0	39.0	33.0	39.0
10	36.949732051515845	39.0	37.0	39.0	33.0	39.0
11	37.013200092622846	39.0	37.0	39.0	33.0	39.0
12	36.83070554330626	39.0	37.0	39.0	32.0	39.0
13	36.8887079273285	39.0	37.0	39.0	32.0	39.0
14	37.7328999347041	40.0	37.0	41.0	32.0	41.0
15	37.74039559367662	39.0	37.0	41.0	32.0	41.0
16	37.624416227394434	39.0	36.0	41.0	32.0	41.0
17	37.8162364321166	39.0	37.0	41.0	32.0	41.0
18	37.83005392125668	40.0	37.0	41.0	32.0	41.0
19	37.95387013228703	40.0	37.0	41.0	33.0	41.0
20	37.86745280218872	40.0	37.0	41.0	32.0	41.0
21	37.75180793700791	40.0	37.0	41.0	32.0	41.0
22	37.66983698485895	40.0	37.0	41.0	32.0	41.0
23	37.45996307920697	39.0	36.0	41.0	32.0	41.0
24	37.54969600775208	39.0	36.0	41.0	32.0	41.0
25	37.48695659614246	39.0	36.0	41.0	32.0	41.0
26	37.22968701285436	39.0	36.0	41.0	31.0	41.0
27	37.19138409657162	39.0	35.0	41.0	31.0	41.0
28	36.99558971929181	39.0	35.0	41.0	31.0	41.0
29	36.741949646755074	39.0	35.0	41.0	30.0	41.0
30	36.4895903578119	38.0	35.0	40.0	30.0	41.0
31	36.06656598655899	38.0	35.0	40.0	30.0	41.0
32	35.87980768459461	38.0	35.0	40.0	30.0	41.0
33	35.64418869177715	38.0	35.0	40.0	29.0	41.0
34	35.39979994106791	38.0	34.0	40.0	27.0	41.0
35	35.15422345743119	38.0	34.0	40.0	26.0	41.0
36	34.97598169789657	38.0	34.0	40.0	25.0	41.0
37	34.72538743035238	38.0	33.0	40.0	24.0	41.0
38	34.519315767435366	37.0	33.0	40.0	23.0	41.0
39	34.293763894095505	37.0	33.0	40.0	22.0	41.0
40	33.980184218927896	37.0	33.0	40.0	20.0	41.0
41	33.70794524342642	37.0	33.0	40.0	18.0	41.0
42	33.24473047125884	37.0	33.0	40.0	15.0	41.0
43	32.76731183577734	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	7.0
15	13.0
16	45.0
17	96.0
18	231.0
19	639.0
20	1359.0
21	2768.0
22	5386.0
23	9474.0
24	15480.0
25	24070.0
26	35681.0
27	50909.0
28	69624.0
29	90286.0
30	110358.0
31	131362.0
32	157640.0
33	192299.0
34	236726.0
35	289367.0
36	384917.0
37	590499.0
38	645392.0
39	695262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.805311267847415	21.226621688612898	17.2237585089219	19.744308534617787
2	18.760832514683557	25.664149129200847	35.622308415897976	19.952709940217623
3	19.46210832487945	23.760594540278905	36.69538580998733	20.081911324854314
4	12.197257786174918	15.878160313463093	35.28334195210672	36.64123994825527
5	10.557988479899718	40.523244216820885	34.24769873402965	14.671068569249748
6	30.89169811583097	38.25331599952726	11.294987189490195	19.559998695151577
7	22.997953420120016	37.08817727114483	19.17715402933449	20.73671527940067
8	29.197855128669286	33.0282804504294	17.37646320835488	20.397401212546438
9	23.380209834599768	14.197579720088624	23.326277882444924	39.095932562866686
10	16.51772321063302	29.490831033359733	30.970871398096953	23.020574357910295
11	31.865815591762125	22.663639489633137	24.33911299185165	21.131431926753084
12	22.92129357538628	29.90883708593122	29.993999836359176	17.175869502323327
13	35.206896016950196	21.329084376097956	26.53144551001124	16.932574096940613
14	19.460584219141804	24.704684459308783	30.927982527865733	24.90674879368368
15	20.680296628410723	31.572278174431496	28.038839561934576	19.708585635223205
16	19.27825304326482	29.077236865818268	28.350345383751108	23.29416470716581
17	20.91677366601977	26.852042488859322	27.5129161276591	24.718267717461814
18	23.492512362636628	23.640778438342437	32.994562953321186	19.87214624569975
19	22.337213474806	22.917068861236324	35.72701715394377	19.018700510013907
20	23.50387630892607	22.000172197209654	35.013896100909705	19.482055392954567
21	21.290179571742332	23.479009320575052	37.162858452289335	18.067952655393277
22	18.33306683765172	24.333150262386834	36.17654813045703	21.15723476950441
23	21.015733584177966	24.367482749529533	37.16331301014092	17.453470656151584
24	19.938190827664286	24.546738975234085	33.77362163353118	21.74144856357045
25	19.942977054454424	25.208013694491132	35.728755169258626	19.120254081795814
26	19.01573251463008	27.16336113979579	35.33270158704863	18.4882047585255
27	16.850833899748068	26.824715540370352	34.93934861325095	21.38510194663063
28	18.47312413333198	26.025977179056753	34.471394674400216	21.029504013211056
29	18.62390364657004	25.729445027645138	32.893303507207946	22.75334781857687
30	19.12316859978534	26.250769406010537	36.8249882750813	17.80107371912282
31	21.0588898413807	26.28175955600928	35.3650019332078	17.29434866940221
32	19.897227143627983	26.026886294759912	33.78568078594657	20.29020577566554
33	18.968699146340352	25.626206917942685	35.20133436794266	20.203759567774306
34	17.239213475875545	27.05629939640065	36.387275802709055	19.317211325014746
35	16.253384450591966	31.1059018187127	34.048736088524336	18.59197764217099
36	21.121511870109828	27.00587021357267	33.27801988182566	18.59459803449185
37	19.580213150198215	29.329409518655318	32.609525714337664	18.4808516168088
38	18.988967078781293	26.0234102641302	35.09454001151903	19.89308264556948
39	18.24055094550707	25.817655989548378	36.08200009732886	19.859792967615693
40	15.972173572648238	26.37411501597102	35.830709821497805	21.82300158988293
41	18.564730909773473	25.40264467105787	36.004992649532156	20.027631769636496
42	17.489968977763564	28.536928012475204	34.286122241836544	19.686980767924688
43	17.435662683848598	29.122879821856102	32.822098356693154	20.619359137602146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1298.0
1	1206.5
2	1115.0
3	2823.5
4	4532.0
5	4532.0
6	7582.5
7	10633.0
8	11860.5
9	13088.0
10	20728.0
11	28368.0
12	28368.0
13	53765.0
14	79162.0
15	145120.0
16	211078.0
17	188565.0
18	166052.0
19	166052.0
20	161721.5
21	157391.0
22	113892.5
23	70394.0
24	72969.5
25	75545.0
26	75545.0
27	77652.0
28	79759.0
29	78890.5
30	78022.0
31	80672.5
32	83323.0
33	83323.0
34	90725.0
35	98127.0
36	108603.5
37	119080.0
38	125580.5
39	132081.0
40	132081.0
41	136630.0
42	141179.0
43	150906.0
44	160633.0
45	194720.0
46	228807.0
47	228807.0
48	382723.0
49	536639.0
50	475028.5
51	413418.0
52	350585.0
53	287752.0
54	287752.0
55	239872.0
56	191992.0
57	150460.5
58	108929.0
59	93281.5
60	77634.0
61	77634.0
62	66894.5
63	56155.0
64	48774.5
65	41394.0
66	35608.5
67	29823.0
68	29823.0
69	25682.5
70	21542.0
71	18092.5
72	14643.0
73	11831.0
74	9019.0
75	9019.0
76	7157.0
77	5295.0
78	4116.0
79	2937.0
80	2270.0
81	1603.0
82	1603.0
83	1176.5
84	750.0
85	577.0
86	404.0
87	285.0
88	166.0
89	166.0
90	129.5
91	93.0
92	62.0
93	31.0
94	20.0
95	9.0
96	9.0
97	5.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3739898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.564756100794376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.56144316577533	24.606399673175098
2	12.403339563370736	8.078234554330027
3	4.440525857471587	4.338139245234991
4	2.1029493892526454	2.7392813581330757
5	1.1951966456572385	1.9460643629157763
6	0.7878650214360476	1.5393979358047214
7	0.5215881209155732	1.1889772959881098
8	0.3870236932604824	1.0082665741005012
9	0.29614039946026	0.8679365892013666
>10	1.93145502310125	12.265691687265502
>50	0.20939361253350317	4.748578232207334
>100	0.13667309965665414	8.30535092169721
>500	0.012130443724877333	2.743166056621053
>1k	0.01130524347147355	7.765142320498673
>5k	0.001732920532123683	4.002516318444489
>10k+	0.001237800380088345	13.856856874382196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	119880	3.2054350145378296	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	85342	2.28193389231471	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	85332	2.281666505343194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33008	0.88259091558112	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	27927	0.7467315953536701	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	22907	0.6125033356524696	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	22577	0.6036795655924306	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	22292	0.596059036904215	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	18662	0.4989975662437853	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18347	0.4905748766410207	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13826	0.36968922681848543	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	13422	0.35888679316922545	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	11106	0.2969599705660422	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	10620	0.2839649637503483	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10405	0.27821614386274707	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	9577	0.25607650262119447	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	9554	0.2554615125867069	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8472	0.2265302422686394	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	8277	0.22131619632407085	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	8261	0.2208883771696447	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	7871	0.21046028528050767	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	7710	0.20615535503909466	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	7662	0.20487189757581623	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	7599	0.20318735965526333	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7273	0.19447054438383077	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	7089	0.1895506241079302	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6712	0.17947013528176436	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	6667	0.17826689390994085	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	6210	0.16604730931164433	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	6146	0.1643360326939398	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6092	0.16289214304775157	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	5847	0.15634116224560135	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5569	0.14890780443744722	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5531	0.14789173394568514	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	5511	0.1473569600026525	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5315	0.1421161753609323	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4971	0.13291806354077035	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4756	0.12716924365316915	No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA	4629	0.12377342911491168	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4461	0.11928132799343726	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	4411	0.11794439313585557	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4404	0.11775722225579414	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	4284	0.11454857859759812	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA	4044	0.10813129128120606	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	4034	0.10786390430968973	No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	4030	0.1077569495210832	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3983	0.10650023075495642	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	3825	0.10227551660499833	No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA	3790	0.10133966220469114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.6738697151633548E-5	0.0	0.0	0.0	0.0
7	2.6738697151633548E-5	0.0	0.0	0.0	0.0
8	5.3477394303267097E-5	0.0	0.0	2.6738697151633548E-5	0.0
9	5.3477394303267097E-5	0.0	0.0	1.0695478860653419E-4	0.0
10	1.3369348575816773E-4	0.0	0.0	2.1390957721306839E-4	0.0
11	1.3369348575816773E-4	0.0	0.0	6.417287316392051E-4	0.0
12	1.3369348575816773E-4	0.0	0.0	0.0011765026746718762	0.0
13	1.6043218290980128E-4	0.0	0.0	0.0012834574632784102	0.0
14	1.8717088006143482E-4	0.0	0.0	0.0017914927091594477	0.0
15	1.8717088006143482E-4	0.0	0.0	0.0025669149265568204	0.0
16	2.1390957721306839E-4	0.0	0.0	0.003716678904077063	0.0
17	2.1390957721306839E-4	0.0	0.0	0.00542795552178161	0.0
18	2.4064827436470193E-4	0.0	0.0	0.006657935590756754	0.0
19	2.4064827436470193E-4	0.0	0.0	0.008797031362887437	0.0
20	2.6738697151633547E-4	0.0	0.0	0.014305202976123949	0.0
21	2.6738697151633547E-4	0.0	0.0	0.02751411936903092	0.0
22	2.6738697151633547E-4	0.0	0.0	0.043610815054314314	0.0
23	2.6738697151633547E-4	0.0	0.0	0.05278218817732462	0.0
24	2.6738697151633547E-4	0.0	0.0	0.06441352143828522	0.0
25	2.6738697151633547E-4	0.0	0.0	0.06968104477715703	0.0
26	2.6738697151633547E-4	0.0	0.0	0.07807699568276996	0.0
27	2.6738697151633547E-4	0.0	0.0	0.13404108882113897	0.0
28	2.6738697151633547E-4	0.0	0.0	0.2813713101266398	0.0
29	2.6738697151633547E-4	0.0	0.0	0.5348006817298225	0.0
30	2.6738697151633547E-4	0.0	0.0	0.9204796494449848	0.0
31	2.6738697151633547E-4	0.0	0.0	1.3144476132771536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1100	0.0	25.73182	1
GGTATCA	25300	0.0	24.86166	1
ATTGGAC	1175	0.0	24.404255	3
TTGGACC	2110	0.0	20.165876	4
TATTGGA	1455	0.0	19.580755	2
GGACCCT	2120	0.0	19.372643	6
TTTTTAC	3520	0.0	19.235796	1
TGGACCC	2225	0.0	19.123596	5
CCGTTTA	90	2.1548567E-6	18.5	27
ACTCTGT	2515	0.0	17.654076	37
GACCCTC	2310	0.0	17.619047	7
CGCCGTT	100	5.88492E-6	16.650002	25
TACCGAC	775	0.0	16.23226	7
CTCTAAT	265	0.0	16.056604	1
ACGGTGT	185	1.8189894E-11	16.0	29
GATACCT	2590	0.0	15.928572	36
AGCCGTC	175	1.3278623E-10	15.857143	12
CGTTTAC	105	9.354289E-6	15.857142	28
GTTTAGA	165	9.767973E-10	15.69697	1
ACCGACG	645	0.0	15.488372	8
>>END_MODULE