FastQCFastQC Report
Wed 25 May 2016
SRR2088521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955023
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440822.2548072324468817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310111.5862217477748342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300511.537117466137227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121360.6207599603687527No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78990.40403616734943787No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63770.3261854208364812No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA63140.32296295235401323No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61370.3139093504270794No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA59390.3037815923393229No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG45510.23278498513828227No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44380.2270050019871889No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39590.20250401146175773No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC37660.19263200484086376No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT34760.17779841976283656No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT31120.15917971297524378No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29830.1525813251301903No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27040.13831039327926065No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG25830.13212120778118724No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG25520.1305355486866395No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25120.12848953695173917No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA24980.12777343284452408No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT24900.12736423049754403No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.1259831725764863No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA23680.12112389470609809No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT23280.11907788297119778No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22110.11309329864661438No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21460.1097685295774014No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT21430.10961507869728386No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC20180.10322129202572042No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA20050.10255633821187782No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA19660.10056147677035002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96900.023.8266261
GTATTGG5500.022.1999991
TATACCG1303.274181E-1119.9230775
ATTGGAC6000.019.7333323
GGACCCT7700.018.9805186
CGACGTT701.2197522E-418.522
ACGTTTA1103.855712E-818.526
TTGGACC8150.018.1595124
CGAACTA1552.1827873E-1117.90322724
GACCCTC8550.016.8771937
ATACGTC1107.8144876E-716.8181825
TATACAC12250.016.61224637
TAAACGT1151.2432083E-616.0869564
CGATTAA1504.6766218E-916.03333324
TATACGT1504.6766218E-916.0333334
TCGGGTA700.002593365515.85714225
TCTAACG1403.4768163E-815.8571422
AAACCGT700.002593365515.8571426
TGGACCC9350.015.6310165
TACCGAC4000.015.2625017
Produced by FastQC (version 0.11.5)