FastQCFastQC Report
Wed 25 May 2016
SRR2088520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785508
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT708481.871558586060312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT508561.34343924250061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT503041.3288573158477013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202300.5344064786020792No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG123610.3265347741967524No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA121320.32048538795849857No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG103000.2720902980524675No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA101200.26733532196999715No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA94720.25021740807310405No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG85370.22551794897804994No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA73470.19408227376616294No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC67950.17950034711325402No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61880.16346551110181248No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56740.14988741273298062No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT55760.14729859242141344No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT51500.13604514902623374No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47810.1262974480571696No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA44630.11789699031147206No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.11739507616943354No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG41920.1107381096539751No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG41480.10957578216714903No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT40840.10788512400449293No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT39940.10550763596325777No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38260.10106965828628549No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37930.10019791267116593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161100.023.5297971
GTATTGG11350.020.8634361
ATTGGAC11000.019.8454553
TTGGACC17050.019.2052784
ACGTTTA2800.019.16071526
GGACCCT16650.018.888896
CCGTTTA1451.546141E-1017.86206827
TGGACCC19100.016.6596855
GACCCTC17550.016.6552727
TATACCG2750.016.1454545
CGTTTAC1701.4897523E-915.23529328
GGACCGT3700.015.06
TCTACGC750.00410671914.83
TATTGGA14700.014.7244892
CCCTCGC19550.014.66752059
GTATCAA259500.014.6146432
TTTTTAC22700.014.5881051
ACCCTCG20350.014.5454558
CCGTATA1551.2123928E-714.322582
CTGTGCG3300.014.0151519
Produced by FastQC (version 0.11.5)