##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3785508
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75370967384034	31.0	31.0	34.0	30.0	34.0
2	31.90554715509781	31.0	31.0	34.0	30.0	34.0
3	32.01851006522771	33.0	31.0	34.0	30.0	34.0
4	35.68854827410218	37.0	35.0	37.0	35.0	37.0
5	35.61097083931668	37.0	35.0	37.0	33.0	37.0
6	35.66405618479739	37.0	35.0	37.0	33.0	37.0
7	35.52027944466106	37.0	35.0	37.0	33.0	37.0
8	35.562211729575	37.0	35.0	37.0	33.0	37.0
9	37.163598386266784	39.0	37.0	39.0	33.0	39.0
10	37.03528747000403	39.0	37.0	39.0	33.0	39.0
11	37.09026371097353	39.0	37.0	39.0	33.0	39.0
12	36.94093580042626	39.0	37.0	39.0	33.0	39.0
13	37.00325504529379	39.0	37.0	39.0	33.0	39.0
14	38.01260913990936	40.0	37.0	41.0	33.0	41.0
15	38.016163484531006	40.0	37.0	41.0	33.0	41.0
16	37.90442973571843	40.0	37.0	41.0	33.0	41.0
17	37.96727440544307	40.0	37.0	41.0	33.0	41.0
18	37.943633985187724	40.0	37.0	41.0	33.0	41.0
19	38.012866965279166	40.0	37.0	41.0	33.0	41.0
20	37.92084470565113	40.0	37.0	41.0	32.0	41.0
21	37.79811084800244	40.0	37.0	41.0	32.0	41.0
22	37.69074771470566	40.0	37.0	41.0	32.0	41.0
23	37.53547581988996	39.0	36.0	41.0	32.0	41.0
24	37.67817925625834	40.0	37.0	41.0	32.0	41.0
25	37.62323075264931	40.0	37.0	41.0	32.0	41.0
26	37.4183752880723	39.0	36.0	41.0	32.0	41.0
27	37.3825703181713	39.0	36.0	41.0	32.0	41.0
28	37.23068740047571	39.0	36.0	41.0	31.0	41.0
29	37.01491926578942	39.0	36.0	41.0	31.0	41.0
30	36.874290848150366	39.0	35.0	41.0	31.0	41.0
31	36.6043854616078	39.0	35.0	40.0	30.0	41.0
32	36.4455394097701	38.0	35.0	40.0	30.0	41.0
33	36.27232910351794	38.0	35.0	40.0	30.0	41.0
34	36.08353013651008	38.0	35.0	40.0	30.0	41.0
35	35.87986051013497	38.0	35.0	40.0	29.0	41.0
36	35.785528124626865	38.0	35.0	40.0	28.0	41.0
37	35.63495335368463	38.0	35.0	40.0	27.0	41.0
38	35.478721217865605	38.0	35.0	40.0	26.0	41.0
39	35.33565587498428	38.0	35.0	40.0	25.0	41.0
40	35.133189521723374	38.0	34.0	40.0	24.0	41.0
41	34.973816724201875	38.0	34.0	40.0	24.0	41.0
42	34.66407758218976	38.0	34.0	40.0	22.0	41.0
43	34.25813100910102	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	7.0
14	9.0
15	19.0
16	45.0
17	93.0
18	226.0
19	513.0
20	1132.0
21	2411.0
22	4474.0
23	7721.0
24	12700.0
25	19698.0
26	29373.0
27	41430.0
28	56782.0
29	75090.0
30	95132.0
31	117571.0
32	144145.0
33	178048.0
34	222103.0
35	276857.0
36	366515.0
37	544261.0
38	704193.0
39	884956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.534768913445696	21.062536388775296	14.96673630064974	22.435958397129262
2	18.293264734878385	24.08717667483466	35.6846425895811	21.93491600070585
3	18.989498899487202	24.291719895982254	34.1236103582399	22.595170846290642
4	12.993209894154232	16.35238916414917	36.2743388734088	34.3800620682878
5	12.023485355201997	39.21338958998369	34.78389162035848	13.979233434455823
6	31.663385733169765	38.23262293990661	13.413708279047357	16.69028304787627
7	25.12524078670551	34.56297014826015	20.966406622308025	19.345382442726315
8	27.653725735092888	34.20307129188473	18.73093386673598	19.412269106286395
9	24.42971986850906	14.553211880677575	22.108921708790472	38.90814654202289
10	16.26936199844248	28.687748117293637	32.71259762230063	22.330292261963255
11	33.0437288733771	23.009831177215844	23.474841421547648	20.471598527859406
12	21.79791985646312	28.200019653901144	30.536139403218804	19.465921086416934
13	32.39142540446355	21.38909757950584	25.91628389109203	20.30319312493858
14	20.331273900358948	23.402803533898226	28.97151452328195	27.294408042460876
15	22.175121542471974	30.69572168385326	25.678825668840222	21.45033110483454
16	21.05643416946946	28.744015334269534	26.897314706507025	23.30223578975398
17	21.459418392458822	27.463473858726488	27.256764481807988	23.8203432670067
18	22.769731301584887	25.599734566668463	30.434145166249817	21.196388965496837
19	22.447660921598896	24.969436070403233	32.07831022943288	20.50459277856499
20	23.239443688931576	24.659358796758585	31.367546971238735	20.733650543071104
21	21.958373882712703	25.462210091749903	32.25928990243846	20.320126123098934
22	20.597394061774533	26.134801458615332	31.601782376367982	21.666022103242156
23	21.84631494636915	26.135910953034575	32.050097371343554	19.967676729252716
24	21.222937581957297	26.26168007041591	30.379621440504156	22.135760907122638
25	21.42462781745541	26.30188603484658	31.5429791721481	20.73050697554991
26	20.59982438288335	27.526239543015095	31.478152998223752	20.395783075877798
27	19.588863634682586	27.297525193448273	31.193065765545867	21.92054540632327
28	20.428698076982005	27.053225088944473	30.99243219139941	21.525644642674113
29	20.50567585645044	27.054545915634044	30.19343771034165	22.246340517573863
30	20.66362031198983	27.34370129451582	32.07189101172154	19.920787381772804
31	21.60352058429146	27.219675668364722	31.496089824668182	19.680713922675636
32	20.833663540005727	27.07781888190436	30.625136705562372	21.463380872527544
33	20.38651087251698	26.8684942681405	31.430708903534217	21.31428595580831
34	19.703564224405284	27.30230658606454	32.25345184847054	20.74067734105964
35	19.119547495342765	29.507083329370854	31.01258800668233	20.360781168604056
36	21.53246010839232	27.431324937102232	30.62392154500796	20.412293409497483
37	20.543055251765416	28.325551022478358	30.48034768385115	20.651046041905076
38	20.215886480757668	26.657558245815355	31.91912419680529	21.207431076621685
39	19.731090252616028	26.425488996457013	32.740625564653406	21.102795186273546
40	18.53138337047498	26.704289094092527	32.67115008078176	22.093177454650736
41	19.70810786821742	25.88712003778621	32.94001756171166	21.4647545322847
42	18.862646704220413	27.663024354987492	31.972142180124834	21.50218676066726
43	18.722876823929578	27.50183066579175	31.573067604136618	22.202224906142057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1681.0
1	1464.0
2	1247.0
3	2792.0
4	4337.0
5	4337.0
6	6367.0
7	8397.0
8	9058.5
9	9720.0
10	15045.0
11	20370.0
12	20370.0
13	36418.5
14	52467.0
15	91376.5
16	130286.0
17	118863.5
18	107441.0
19	107441.0
20	108115.0
21	108789.0
22	85834.5
23	62880.0
24	68990.0
25	75100.0
26	75100.0
27	82368.0
28	89636.0
29	94890.5
30	100145.0
31	107863.0
32	115581.0
33	115581.0
34	126594.0
35	137607.0
36	149949.0
37	162291.0
38	171284.0
39	180277.0
40	180277.0
41	186037.0
42	191797.0
43	201513.5
44	211230.0
45	227578.5
46	243927.0
47	243927.0
48	321472.5
49	399018.0
50	369420.5
51	339823.0
52	303100.0
53	266377.0
54	266377.0
55	237482.5
56	208588.0
57	178100.5
58	147613.0
59	132076.5
60	116540.0
61	116540.0
62	103042.0
63	89544.0
64	77812.0
65	66080.0
66	56407.0
67	46734.0
68	46734.0
69	39957.0
70	33180.0
71	27942.0
72	22704.0
73	18632.5
74	14561.0
75	14561.0
76	11678.5
77	8796.0
78	6936.0
79	5076.0
80	3985.0
81	2894.0
82	2894.0
83	2141.5
84	1389.0
85	1035.0
86	681.0
87	533.0
88	385.0
89	385.0
90	288.0
91	191.0
92	133.5
93	76.0
94	53.0
95	30.0
96	30.0
97	24.0
98	18.0
99	11.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3785508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.03243201623864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84198156354972	31.457905020304892
2	12.957918435987366	10.893056514652086
3	4.824756809577046	6.0838878778029475
4	2.2742911548057148	3.823759533980162
5	1.238941916395204	2.603787093647491
6	0.7807699866620355	1.9690596830814933
7	0.5078370666231988	1.494193888471596
8	0.35018138992303827	1.177518037223365
9	0.29121414766063225	1.1016394977341164
>10	1.674217988995982	13.463463919276727
>50	0.15373890637235074	4.4751804326418725
>100	0.09097181516127804	7.0563659786640285
>500	0.006873163606583187	1.924894042397758
>1k	0.005296749931209836	4.738977493799954
>5k	5.044523744009368E-4	1.4508134128618726
>10k+	5.044523744009368E-4	6.285497573459617
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	70848	1.871558586060312	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	50856	1.34343924250061	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50304	1.3288573158477013	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20230	0.5344064786020792	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12361	0.3265347741967524	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12132	0.32048538795849857	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10300	0.2720902980524675	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10120	0.26733532196999715	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9472	0.25021740807310405	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8537	0.22551794897804994	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7347	0.19408227376616294	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6795	0.17950034711325402	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6188	0.16346551110181248	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5674	0.14988741273298062	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5576	0.14729859242141344	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5150	0.13604514902623374	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4781	0.1262974480571696	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4463	0.11789699031147206	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4444	0.11739507616943354	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4192	0.1107381096539751	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4148	0.10957578216714903	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4084	0.10788512400449293	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3994	0.10550763596325777	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3826	0.10106965828628549	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3793	0.10019791267116593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.6416533791501695E-5	0.0	0.0	0.0
3	0.0	2.6416533791501695E-5	0.0	0.0	0.0
4	0.0	2.6416533791501695E-5	0.0	0.0	0.0
5	0.0	7.924960137450508E-5	0.0	0.0	0.0
6	0.0	7.924960137450508E-5	0.0	0.0	0.0
7	0.0	2.6416533791501695E-4	0.0	0.0	0.0
8	0.0	2.6416533791501695E-4	0.0	0.0	0.0
9	0.0	2.6416533791501695E-4	0.0	5.283306758300339E-5	0.0
10	0.0	2.6416533791501695E-4	0.0	5.283306758300339E-5	0.0
11	2.6416533791501695E-5	2.6416533791501695E-4	0.0	1.3208266895750847E-4	0.0
12	2.6416533791501695E-5	2.6416533791501695E-4	0.0	2.6416533791501695E-4	0.0
13	2.6416533791501695E-5	2.6416533791501695E-4	0.0	3.1699840549802034E-4	0.0
14	2.6416533791501695E-5	2.6416533791501695E-4	0.0	3.698314730810237E-4	0.0
15	2.6416533791501695E-5	2.6416533791501695E-4	0.0	4.754976082470305E-4	0.0
16	5.283306758300339E-5	2.6416533791501695E-4	0.0	7.132464123705458E-4	0.0
17	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.001030244817868566	0.0
18	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.001215160554409078	0.0
19	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.0016114085612816034	0.0
20	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.0033284832577292135	0.0
21	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.006049386238253888	0.0
22	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.010328864712477162	0.0
23	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.01262710315233781	0.0
24	5.283306758300339E-5	2.9058187170651864E-4	0.0	0.016431084018314054	0.0
25	5.283306758300339E-5	3.962480068725254E-4	0.0	0.01946898540433675	0.0
26	5.283306758300339E-5	3.962480068725254E-4	0.0	0.02480512523022009	0.0
27	5.283306758300339E-5	3.962480068725254E-4	0.0	0.06836598945240639	0.0
28	5.283306758300339E-5	3.962480068725254E-4	0.0	0.17881351723467498	0.0
29	5.283306758300339E-5	3.962480068725254E-4	0.0	0.3632009230993568	0.0
30	5.283306758300339E-5	3.962480068725254E-4	0.0	0.607738776407288	0.0
31	5.283306758300339E-5	3.962480068725254E-4	0.0	0.9364132898411521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16110	0.0	23.529797	1
GTATTGG	1135	0.0	20.863436	1
ATTGGAC	1100	0.0	19.845455	3
TTGGACC	1705	0.0	19.205278	4
ACGTTTA	280	0.0	19.160715	26
GGACCCT	1665	0.0	18.88889	6
CCGTTTA	145	1.546141E-10	17.862068	27
TGGACCC	1910	0.0	16.659685	5
GACCCTC	1755	0.0	16.655272	7
TATACCG	275	0.0	16.145454	5
CGTTTAC	170	1.4897523E-9	15.235293	28
GGACCGT	370	0.0	15.0	6
TCTACGC	75	0.004106719	14.8	3
TATTGGA	1470	0.0	14.724489	2
CCCTCGC	1955	0.0	14.6675205	9
GTATCAA	25950	0.0	14.614643	2
TTTTTAC	2270	0.0	14.588105	1
ACCCTCG	2035	0.0	14.545455	8
CCGTATA	155	1.2123928E-7	14.32258	2
CTGTGCG	330	0.0	14.015151	9
>>END_MODULE