FastQCFastQC Report
Wed 25 May 2016
SRR2088519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790077
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333501.8630483493168168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236111.3189935405013304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207521.1592797404804374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96790.540702997692278No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG50450.2818314519431287No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46660.26065917834819397No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA43360.2422242171705463No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG37820.21127582779958626No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG37220.2079240166763776No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA32680.18256197917743203No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31010.1732327715511679No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.1679257372727542No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25450.1421726551427676No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT20820.11630784597534073No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20320.11351467003933349No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20080.11217394559005003No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT18760.10479996111899097No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.10061019721498013No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA17980.10044260665881971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGT502.7029178E-422.21
GGTATCA80150.021.4198381
TCGAAAT450.00382662920.55555523
TCGCGAC450.00382662920.55555519
TCACGTT1101.7571438E-920.1818224
GTATTGG5050.019.415841
ACGTTTA1205.178663E-918.526
TAAACGT801.617491E-518.54
CGTTTAT1301.3946192E-817.07692327
ATTGGAC5900.016.6186453
AGTCGAC803.3838832E-416.187535
TTGGACC7800.016.1282064
ATTCGTC1059.348842E-615.85714217
CCGTATA957.063188E-515.5789482
GACGTAT1201.936869E-615.41666628
GGACCCT7400.015.256
CGAATTA855.366224E-415.23529415
CGTATAC1101.4526877E-515.1363653
TATACAC7950.015.12578637
TGGACCC8200.014.6646345
Produced by FastQC (version 0.11.5)