##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1790077
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32648539699689	31.0	31.0	33.0	30.0	34.0
2	31.493566477866594	31.0	31.0	34.0	30.0	34.0
3	31.582020214772886	31.0	31.0	34.0	30.0	34.0
4	35.3319320900721	37.0	35.0	37.0	33.0	37.0
5	35.22044303122156	37.0	35.0	37.0	33.0	37.0
6	35.312393824399734	37.0	35.0	37.0	33.0	37.0
7	35.15661225746155	37.0	35.0	37.0	32.0	37.0
8	35.222661930185126	37.0	35.0	37.0	32.0	37.0
9	36.75257377196623	39.0	37.0	39.0	32.0	39.0
10	36.54384196880916	38.0	35.0	39.0	32.0	39.0
11	36.64698222478698	38.0	35.0	39.0	32.0	39.0
12	36.45514522559644	38.0	35.0	39.0	32.0	39.0
13	36.53941701949134	38.0	35.0	39.0	32.0	39.0
14	37.42505210669709	39.0	36.0	41.0	32.0	41.0
15	37.44525738278298	39.0	36.0	41.0	32.0	41.0
16	37.307243207973734	39.0	36.0	40.0	32.0	41.0
17	37.36351341311016	39.0	36.0	40.0	32.0	41.0
18	37.3391647398408	39.0	36.0	40.0	32.0	41.0
19	37.41036223581444	39.0	36.0	40.0	32.0	41.0
20	37.30490978879679	39.0	36.0	40.0	31.0	41.0
21	37.16247345784567	39.0	36.0	40.0	31.0	41.0
22	37.02783176366156	39.0	36.0	40.0	31.0	41.0
23	36.842176621452595	39.0	36.0	40.0	30.0	41.0
24	36.99414047552144	39.0	36.0	40.0	31.0	41.0
25	36.923159171365256	39.0	36.0	40.0	31.0	41.0
26	36.67063651451865	39.0	35.0	40.0	30.0	41.0
27	36.61189323140848	39.0	35.0	40.0	30.0	41.0
28	36.44497862382456	39.0	35.0	40.0	30.0	41.0
29	36.213244458199284	38.0	35.0	40.0	30.0	41.0
30	36.052846888709254	38.0	35.0	40.0	29.0	41.0
31	35.763813511932725	38.0	35.0	40.0	28.0	41.0
32	35.59470067488717	38.0	34.0	40.0	27.0	41.0
33	35.39941522068604	38.0	34.0	40.0	27.0	41.0
34	35.18000398865524	38.0	34.0	40.0	26.0	41.0
35	34.951430580919144	38.0	33.0	40.0	25.0	41.0
36	34.87351382091385	38.0	33.0	40.0	25.0	41.0
37	34.69227301395415	38.0	33.0	40.0	24.0	41.0
38	34.53334688954721	38.0	33.0	40.0	24.0	41.0
39	34.36273299975364	38.0	33.0	40.0	23.0	41.0
40	34.15775019733788	37.0	33.0	40.0	22.0	41.0
41	33.989827811876246	37.0	33.0	40.0	21.0	41.0
42	33.670972254266154	37.0	33.0	40.0	19.0	41.0
43	33.2402840771654	37.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	5.0
15	23.0
16	28.0
17	90.0
18	205.0
19	451.0
20	1054.0
21	1909.0
22	3628.0
23	5815.0
24	9192.0
25	13911.0
26	19378.0
27	26869.0
28	35173.0
29	45314.0
30	55790.0
31	67670.0
32	81700.0
33	98930.0
34	121059.0
35	149512.0
36	197993.0
37	276013.0
38	345670.0
39	232692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.254134319361675	22.51958993942719	15.39660025797773	21.8296754832334
2	19.83277814306312	25.077971506253643	33.84603008697391	21.243220263709325
3	20.611962502171693	24.809044527134866	33.20264994187401	21.37634302881943
4	14.693837192478313	17.415172643411427	35.28909650255269	32.601893661557575
5	12.216290137239906	40.01721713646955	34.24997919083928	13.516513535451269
6	32.10202689604972	38.43778787169491	13.1427865952135	16.31739863704187
7	25.207965914315416	34.52002344033245	21.018928236047945	19.25308240930418
8	27.901928241075662	34.758560665267474	18.4638984803447	18.875612613312164
9	24.817479918461608	14.530436400221891	21.7816887206528	38.8703949606637
10	16.547556334168863	29.000316746151146	32.41100801809084	22.041118901589147
11	33.68290861231109	23.110849421561195	22.855441413972695	20.350800552155018
12	21.269476117507793	28.56949728978139	30.559858598261414	19.6011679944494
13	32.38464043725494	21.102444196534563	25.97011190021435	20.54280346599616
14	20.24298396102514	23.342515433693634	28.802783343956712	27.611717261324515
15	22.568582245344754	30.78806107223321	25.285504478298975	21.357852204123063
16	21.176798539951076	28.70675395527678	26.936327319997964	23.180120184774175
17	21.599908830737448	27.661379929466722	27.483342895305622	23.25536834449021
18	22.455570346973904	25.810900871861936	30.23300115022985	21.500527630934315
19	22.446073548791475	25.264667385816363	31.946502859932842	20.342756205459317
20	23.0742588167995	25.127131402727372	31.192457084248332	20.6061526962248
21	22.04072785695811	25.84291066808858	31.91091779850811	20.205443676445203
22	20.83474621482763	26.467576534417237	31.28412911846809	21.413548132287048
23	21.87190830338583	26.387691702647427	31.72187565115914	20.018524342807602
24	21.427514011967084	26.456292103635764	30.191159374708466	21.92503450968869
25	21.498293090185506	26.677176456655218	31.14145369165684	20.683076761502438
26	20.9469760239364	27.69065241327608	31.085478445899255	20.276893116888267
27	20.101202350513415	27.42446274657459	30.935931806285428	21.53840309662657
28	20.68737825244389	27.3354721612534	30.849399215787926	21.12775037051479
29	20.813350487157813	27.10302405985888	30.119933388340275	21.963692064643027
30	20.79491552598017	27.47865035973313	31.735115305095814	19.99131880919089
31	21.63454421234394	27.27569819622284	31.243125295727502	19.846632295705717
32	20.925077524598105	27.30564104225684	30.42941728204988	21.339864151095174
33	20.71503069421036	27.184249616077967	30.85939878563883	21.24132090407284
34	20.064891063345318	27.454796637239625	31.739919567705748	20.740392731709306
35	19.62317822082514	29.228742674197818	30.713874319372852	20.434204785604194
36	21.725154839708015	27.260447455612248	30.428579329269077	20.585818375410668
37	20.634084455584873	27.976673629123216	30.544551994132096	20.844689921159816
38	20.51347511866808	26.47785542186174	31.763438109086927	21.24523135038325
39	19.926628854512963	26.38897656357799	32.4638549068001	21.22053967510895
40	18.910192131399935	26.768233992169048	32.21637951886986	22.105194357561157
41	19.91003738945308	25.85458614350109	32.56485614864612	21.67052031839971
42	19.10253022635339	27.550211527213637	31.61020447723757	21.737053769195402
43	18.998903399127524	27.385581737545365	31.212232769875264	22.403282093451846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	765.0
1	635.5
2	506.0
3	953.0
4	1400.0
5	1400.0
6	2409.5
7	3419.0
8	3859.5
9	4300.0
10	6682.0
11	9064.0
12	9064.0
13	17089.0
14	25114.0
15	43892.5
16	62671.0
17	55891.0
18	49111.0
19	49111.0
20	50426.5
21	51742.0
22	40839.5
23	29937.0
24	32612.5
25	35288.0
26	35288.0
27	38545.5
28	41803.0
29	44588.0
30	47373.0
31	50961.0
32	54549.0
33	54549.0
34	60205.0
35	65861.0
36	71929.0
37	77997.0
38	82212.0
39	86427.0
40	86427.0
41	89909.5
42	93392.0
43	98429.0
44	103466.0
45	109894.5
46	116323.0
47	116323.0
48	148099.0
49	179875.0
50	166945.5
51	154016.0
52	138493.5
53	122971.0
54	122971.0
55	109884.5
56	96798.0
57	84747.5
58	72697.0
59	65497.5
60	58298.0
61	58298.0
62	51537.5
63	44777.0
64	39104.0
65	33431.0
66	28058.0
67	22685.0
68	22685.0
69	19392.5
70	16100.0
71	13566.5
72	11033.0
73	9129.0
74	7225.0
75	7225.0
76	5751.0
77	4277.0
78	3359.0
79	2441.0
80	1929.5
81	1418.0
82	1418.0
83	1092.0
84	766.0
85	584.5
86	403.0
87	306.0
88	209.0
89	209.0
90	149.5
91	90.0
92	62.5
93	35.0
94	24.0
95	13.0
96	13.0
97	9.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1790077.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.689316463884325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40208898127025	42.076183381741494
2	10.498178753305528	10.852873677480721
3	3.2429317985362776	5.028747840160057
4	1.4037810683744534	2.90241935556867
5	0.7724163166170772	1.996283571574399
6	0.5011653227808252	1.5542935781965683
7	0.3298390551631764	1.1934408713134619
8	0.25207317626595754	1.0423592144054061
9	0.19966229869977725	0.9288366969059448
>10	1.216151555200469	11.905360969475339
>50	0.11138874536313428	3.9370769767050238
>100	0.06123543705767007	5.810183313731007
>500	0.004435561261705555	1.5901910040819853
>1k	0.004111007998653929	4.012692128948733
>5k	2.1636884203441733E-4	0.8233629547456888
>10k+	3.24553263051626E-4	4.345694464965479
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33350	1.8630483493168168	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23611	1.3189935405013304	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20752	1.1592797404804374	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9679	0.540702997692278	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5045	0.2818314519431287	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4666	0.26065917834819397	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4336	0.2422242171705463	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3782	0.21127582779958626	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3722	0.2079240166763776	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3268	0.18256197917743203	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3101	0.1732327715511679	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3006	0.1679257372727542	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2545	0.1421726551427676	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2082	0.11630784597534073	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2032	0.11351467003933349	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2008	0.11217394559005003	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1876	0.10479996111899097	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1801	0.10061019721498013	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1798	0.10044260665881971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.586351872014444E-5	0.0	0.0	0.0	0.0
9	5.586351872014444E-5	0.0	0.0	0.0	0.0
10	5.586351872014444E-5	0.0	0.0	0.0	0.0
11	5.586351872014444E-5	0.0	0.0	1.1172703744028888E-4	0.0
12	5.586351872014444E-5	5.586351872014444E-5	0.0	2.793175936007222E-4	0.0
13	1.1172703744028888E-4	5.586351872014444E-5	0.0	2.793175936007222E-4	0.0
14	1.1172703744028888E-4	5.586351872014444E-5	0.0	2.793175936007222E-4	0.0
15	1.1172703744028888E-4	5.586351872014444E-5	0.0	5.027716684813E-4	0.0
16	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0012289974118431777	0.0
17	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0015641785241640443	0.0
18	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0018434961177647665	0.0
19	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0025138583424064997	0.0
20	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0043573544601712665	0.0
21	1.675905561604333E-4	5.586351872014444E-5	0.0	0.007373984471059066	0.0
22	1.675905561604333E-4	5.586351872014444E-5	0.0	0.012904472824353365	0.0
23	1.675905561604333E-4	5.586351872014444E-5	0.0	0.01569764876036059	0.0
24	1.675905561604333E-4	5.586351872014444E-5	0.0	0.020334320814132577	0.0
25	1.675905561604333E-4	5.586351872014444E-5	0.0	0.0236302684186211	0.0
26	3.351811123208666E-4	5.586351872014444E-5	0.0	0.030501481221198865	0.0
27	3.351811123208666E-4	5.586351872014444E-5	0.0	0.07033217006866185	0.0
28	3.351811123208666E-4	5.586351872014444E-5	0.0	0.17826048823598092	0.0
29	3.351811123208666E-4	5.586351872014444E-5	0.0	0.34372823068504876	0.0
30	3.351811123208666E-4	5.586351872014444E-5	0.0	0.5661208987099438	0.0
31	3.351811123208666E-4	5.586351872014444E-5	0.0	0.8992909243568852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAGT	50	2.7029178E-4	22.2	1
GGTATCA	8015	0.0	21.419838	1
TCGAAAT	45	0.003826629	20.555555	23
TCGCGAC	45	0.003826629	20.555555	19
TCACGTT	110	1.7571438E-9	20.18182	24
GTATTGG	505	0.0	19.41584	1
ACGTTTA	120	5.178663E-9	18.5	26
TAAACGT	80	1.617491E-5	18.5	4
CGTTTAT	130	1.3946192E-8	17.076923	27
ATTGGAC	590	0.0	16.618645	3
AGTCGAC	80	3.3838832E-4	16.1875	35
TTGGACC	780	0.0	16.128206	4
ATTCGTC	105	9.348842E-6	15.857142	17
CCGTATA	95	7.063188E-5	15.578948	2
GACGTAT	120	1.936869E-6	15.416666	28
GGACCCT	740	0.0	15.25	6
CGAATTA	85	5.366224E-4	15.235294	15
CGTATAC	110	1.4526877E-5	15.136365	3
TATACAC	795	0.0	15.125786	37
TGGACCC	820	0.0	14.664634	5
>>END_MODULE