FastQCFastQC Report
Wed 25 May 2016
SRR2088518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4662571
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1146052.4579786559818606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT844201.8105890505474338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT838471.7982996934523894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333880.7160856102781062No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA267120.572902804053815No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG246530.5287426186110624No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG245080.525632746396784No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG201060.43122131545020975No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA200690.4304277618507043No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA167700.3596728071272266No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA149330.3202739432815071No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA141290.30303023803819823No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC132720.2846498208820841No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA110100.23613581433934194No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT103580.22215211307238003No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA85160.1826460122537544No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT84250.18069429934686249No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC82160.17621179387938543No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78060.16741836210108113No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT76830.16478033256758987No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA75120.16111282809419952No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG73700.15806729806366487No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG73500.15763835017204028No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA72640.15579387423805452No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72190.15482874148189915No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG68000.1458422831523638No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG67150.14401925461295922No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT63450.13608371861790416No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC63190.13552608635879218No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT62500.1340462161326873No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT61160.1311722652588025No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG59760.12816963001743029No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA59420.12744041860166846No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT57660.12366567715537201No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA56720.1216496220647364No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55940.11997672528740046No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.10818065826772397No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC49210.10554262873423267No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG48450.10391262674605921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256350.023.4903451
GTATTGG19900.023.0552771
ATTGGAC20600.020.7451463
TATTGGA24200.019.1880172
TGGACCC29550.018.6565155
TTGGACC29750.018.2823524
TTTTTAC38200.017.870421
GGACCCT30250.017.4297526
GTACGAC852.7260068E-517.4117643
GATACTG22550.017.31042136
TATACAC20850.016.68105537
ACTCTGT25250.015.89901137
GACCCTC33300.015.8888897
TGATACT25100.015.69920335
ATACTGT24650.015.68559837
GCGCTAG1954.1836756E-1115.17948711
TATACCG3300.015.1363645
TAGCGAA2151.2732926E-1114.62790710
GATACCT23100.014.33549836
CTAATAC6850.014.31386853
Produced by FastQC (version 0.11.5)