FastQCFastQC Report
Wed 25 May 2016
SRR2088517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4093718
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT798611.9508183025797088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT570451.3934765413738808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT544621.3303798649540588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228120.5572440505183797No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG135980.3321674819809278No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA125880.30749553340019997No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA111110.2714158620598683No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG107750.26320816431420047No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA95720.233821675064086No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95270.2327224298302912No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86800.21203219176308677No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC70660.17260592937764643No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.17255707403392223No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59560.1454912136107079No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT56590.1382361950676622No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT50880.12428799443439925No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49010.11972001979618527No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47080.11500547912679866No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA45550.11126804533189633No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG43540.10635808328761287No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG43070.10520998271009387No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT42550.1039397437732643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188250.021.6988051
GTATTGG13900.021.0287761
ATTGGAC13950.020.025093
TTGGACC18900.018.9894184
GGACCCT18200.018.3983546
TGGACCC20000.017.025
ACCGTTA2550.016.6862748
TATTGGA17150.016.1807582
TATACCG2650.016.0566045
GACCCTC20750.015.9590367
GTACCGA855.3683197E-415.2352946
ATCCGAT855.3683197E-415.2352947
TATACAC19550.015.04603637
TAATACT7600.014.8486844
CTAATAC7900.014.7531643
TGTTACG2151.2732926E-1114.62790716
GATACTG22200.014.41666736
ACTCTGT20600.014.09951537
GTATCGT1051.6572668E-414.0952386
CCGTTTA2900.014.03448427
Produced by FastQC (version 0.11.5)