##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4093718
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.646031553712298	31.0	31.0	34.0	30.0	34.0
2	31.8027480153738	31.0	31.0	34.0	30.0	34.0
3	31.908746279054885	31.0	31.0	34.0	30.0	34.0
4	35.60270932194157	37.0	35.0	37.0	33.0	37.0
5	35.52441985500711	37.0	35.0	37.0	33.0	37.0
6	35.58445916401667	37.0	35.0	37.0	33.0	37.0
7	35.43082303177698	37.0	35.0	37.0	33.0	37.0
8	35.48219002872206	37.0	35.0	37.0	33.0	37.0
9	37.05774848194233	39.0	37.0	39.0	33.0	39.0
10	36.917284238924125	39.0	37.0	39.0	33.0	39.0
11	36.982137509227556	39.0	37.0	39.0	33.0	39.0
12	36.82354646802735	39.0	37.0	39.0	32.0	39.0
13	36.884963742006654	39.0	37.0	39.0	33.0	39.0
14	37.85948177182698	40.0	37.0	41.0	32.0	41.0
15	37.87089902137861	40.0	37.0	41.0	33.0	41.0
16	37.75419264346005	40.0	37.0	41.0	32.0	41.0
17	37.81682617122137	40.0	37.0	41.0	32.0	41.0
18	37.79065534069518	40.0	37.0	41.0	32.0	41.0
19	37.866745095778455	40.0	37.0	41.0	32.0	41.0
20	37.779274488374625	40.0	37.0	41.0	32.0	41.0
21	37.642616565185975	40.0	37.0	41.0	32.0	41.0
22	37.531370993312194	39.0	36.0	41.0	32.0	41.0
23	37.354645092798286	39.0	36.0	41.0	31.0	41.0
24	37.498033083861664	39.0	36.0	41.0	32.0	41.0
25	37.44504799793244	39.0	36.0	41.0	32.0	41.0
26	37.218954016861936	39.0	36.0	41.0	31.0	41.0
27	37.174652724002975	39.0	36.0	41.0	31.0	41.0
28	37.015063568130486	39.0	36.0	41.0	31.0	41.0
29	36.786120587690704	39.0	35.0	41.0	30.0	41.0
30	36.63205868113045	39.0	35.0	40.0	30.0	41.0
31	36.349232653543794	38.0	35.0	40.0	30.0	41.0
32	36.182130522913404	38.0	35.0	40.0	30.0	41.0
33	35.99349735375031	38.0	35.0	40.0	30.0	41.0
34	35.79391863337924	38.0	35.0	40.0	29.0	41.0
35	35.58321555124217	38.0	35.0	40.0	27.0	41.0
36	35.4937360121044	38.0	35.0	40.0	27.0	41.0
37	35.32390311203654	38.0	34.0	40.0	26.0	41.0
38	35.168935427403646	38.0	34.0	40.0	25.0	41.0
39	35.02446871035084	38.0	34.0	40.0	24.0	41.0
40	34.81107907286237	38.0	34.0	40.0	24.0	41.0
41	34.64578971976086	38.0	33.0	40.0	23.0	41.0
42	34.31740486276778	38.0	33.0	40.0	21.0	41.0
43	33.90038688546695	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	9.0
15	25.0
16	52.0
17	115.0
18	272.0
19	710.0
20	1512.0
21	2989.0
22	5510.0
23	9763.0
24	15651.0
25	24232.0
26	35309.0
27	49552.0
28	67230.0
29	87856.0
30	110284.0
31	135021.0
32	164410.0
33	202574.0
34	251313.0
35	309603.0
36	412288.0
37	600375.0
38	771508.0
39	835547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.585668578050566	21.11911470208744	15.012196736560751	22.283019983301244
2	18.76672990176656	24.29119445941318	35.01247521201998	21.929600426800285
3	19.564684230814137	24.183102011423358	33.92424685823499	22.32796689952752
4	13.345545540752928	16.336029985455763	35.97101705588905	34.34740741790226
5	12.159704210206957	39.25700304710779	34.54788532087457	14.035407421810685
6	31.925648029492994	37.991747355338106	13.182417548057781	16.90018706711112
7	25.2807105912034	34.406693377511594	20.76266611427558	19.54992991700943
8	28.059700252924113	33.914964342927384	18.51466075582148	19.510674648327022
9	24.651258342660633	14.31371188733567	21.8414653867218	39.193564383281895
10	16.538462102177043	28.5994052350455	32.31001744624324	22.55211521653421
11	33.340401073058764	22.789943029783682	23.15420847259142	20.71544742456613
12	21.796640608854837	28.18349969392127	30.309024705658764	19.710834991565125
13	32.687424976512794	20.994167160512767	25.855860125196706	20.462547737777737
14	20.46946076891471	23.223754054382837	28.85042399110051	27.456361185601942
15	22.507168300307935	30.413355292181826	25.53475837856931	21.544718028940927
16	21.26555859490077	28.358646101172575	26.811983629551424	23.563811674375227
17	21.618318604261454	27.128737250587363	27.284365948020845	23.968578197130334
18	22.86537568049387	25.217907046845923	30.40097046254774	21.51574681011247
19	22.637441074348548	24.668113436245488	32.08572256320538	20.608722926200585
20	23.424109818995838	24.445943760659624	31.340971703473468	20.78897471687107
21	22.240222702198835	25.15940277273618	32.1699003204422	20.43047420462279
22	20.762959246337925	25.84911808776276	31.612143288814714	21.775779377084596
23	21.9789687516336	25.76792050649312	32.145545931595684	20.1075648102776
24	21.411587217292446	25.999519263417753	30.302600227959033	22.286293291330768
25	21.546867664064795	26.024142356654757	31.52708613539086	20.90190384388959
26	20.89701830951717	27.205659989281138	31.448038189245082	20.44928351195661
27	19.89665629142017	27.02880364499948	31.155956516789875	21.918583546790472
28	20.601516763001264	26.815843201705636	30.993878913984794	21.588761121308305
29	20.73381703380643	26.792978900842705	30.101487205518307	22.371716859832553
30	20.75953937227723	27.1242669866366	32.09192719185835	20.024266449227817
31	21.78662526339137	26.94447932173149	31.46376960992428	19.80512580495286
32	20.96893337547921	26.921810442243455	30.517930155423507	21.591326026853828
33	20.599928964330225	26.730737192938058	31.360660406994327	21.30867343573739
34	19.909871661897572	27.164621500552798	31.959529210365734	20.965977627183896
35	19.291704997755097	29.398434381655015	30.848021285296152	20.461839335293735
36	21.735058448090463	27.233189975469728	30.486516169408834	20.54523540703097
37	20.694439626764716	28.136696274633476	30.413575141228584	20.755288957373224
38	20.40077015563847	26.55810683588855	31.717866252633915	21.32325675583907
39	19.90777088211743	26.332737135288752	32.45572850890071	21.303763473693106
40	18.776623108870712	26.662559560770916	32.39570971913551	22.165107611222854
41	19.975044690425673	25.672408309512285	32.643137607426794	21.70940939263525
42	19.0709765548091	27.508245560637057	31.749182527961135	21.67159535659271
43	18.98848430693077	27.36693147891477	31.286913265642625	22.357670948511842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1399.0
1	1258.0
2	1117.0
3	2567.0
4	4017.0
5	4017.0
6	6378.5
7	8740.0
8	9500.5
9	10261.0
10	15596.0
11	20931.0
12	20931.0
13	39148.0
14	57365.0
15	101007.0
16	144649.0
17	130168.5
18	115688.0
19	115688.0
20	117208.5
21	118729.0
22	91744.0
23	64759.0
24	70816.5
25	76874.0
26	76874.0
27	84234.5
28	91595.0
29	97615.0
30	103635.0
31	110778.0
32	117921.0
33	117921.0
34	130359.5
35	142798.0
36	156909.5
37	171021.0
38	179845.0
39	188669.0
40	188669.0
41	195263.5
42	201858.0
43	213320.0
44	224782.0
45	242775.0
46	260768.0
47	260768.0
48	344712.0
49	428656.0
50	399292.5
51	369929.0
52	333531.0
53	297133.0
54	297133.0
55	263933.0
56	230733.0
57	198939.5
58	167146.0
59	150312.5
60	133479.0
61	133479.0
62	118523.5
63	103568.0
64	90111.0
65	76654.0
66	65303.0
67	53952.0
68	53952.0
69	46095.5
70	38239.0
71	32504.5
72	26770.0
73	21906.5
74	17043.0
75	17043.0
76	13632.5
77	10222.0
78	8024.5
79	5827.0
80	4629.0
81	3431.0
82	3431.0
83	2538.0
84	1645.0
85	1280.0
86	915.0
87	669.5
88	424.0
89	424.0
90	322.0
91	220.0
92	150.5
93	81.0
94	64.5
95	48.0
96	48.0
97	33.5
98	19.0
99	13.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4093718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.9019645884568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34938443637613	31.572872384150504
2	12.772548364250353	10.7038973852634
3	4.654337273100535	5.850776268005796
4	2.2240886623921265	3.727747374925726
5	1.2394944404474344	2.5968626075608734
6	0.7649725918705075	1.9232312673418812
7	0.49782017520789135	1.4601750347086295
8	0.36466794125602525	1.2224242528844331
9	0.26549813747227435	1.0012404199198026
>10	1.5935979820385522	12.689027204115611
>50	0.1610182348617914	4.691331685320225
>100	0.09890665900958734	7.817424342951121
>500	0.007358131702840922	2.0451660168765597
>1k	0.005372604085979374	4.846328195727239
>5k	4.6718296399820647E-4	1.4342271422698214
>10k+	4.6718296399820647E-4	6.417268417978319
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	79861	1.9508183025797088	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	57045	1.3934765413738808	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	54462	1.3303798649540588	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22812	0.5572440505183797	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13598	0.3321674819809278	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12588	0.30749553340019997	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11111	0.2714158620598683	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10775	0.26320816431420047	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9572	0.233821675064086	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9527	0.2327224298302912	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8680	0.21203219176308677	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7066	0.17260592937764643	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7064	0.17255707403392223	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5956	0.1454912136107079	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5659	0.1382361950676622	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5088	0.12428799443439925	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4901	0.11972001979618527	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4708	0.11500547912679866	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4555	0.11126804533189633	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4354	0.10635808328761287	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4307	0.10520998271009387	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4255	0.1039397437732643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.442767186210677E-5	0.0	0.0	0.0	0.0
6	4.885534372421354E-5	0.0	0.0	0.0	0.0
7	4.885534372421354E-5	0.0	0.0	0.0	0.0
8	4.885534372421354E-5	0.0	0.0	0.0	0.0
9	4.885534372421354E-5	0.0	0.0	2.442767186210677E-5	0.0
10	7.32830155863203E-5	0.0	0.0	7.32830155863203E-5	0.0
11	7.32830155863203E-5	0.0	0.0	1.465660311726406E-4	0.0
12	7.32830155863203E-5	0.0	0.0	2.1984904675896092E-4	0.0
13	7.32830155863203E-5	0.0	0.0	3.17559734207388E-4	0.0
14	1.465660311726406E-4	0.0	0.0	3.908427497937083E-4	0.0
15	1.465660311726406E-4	0.0	0.0	5.37408780966349E-4	0.0
16	1.465660311726406E-4	0.0	0.0	7.084024840010963E-4	0.0
17	1.465660311726406E-4	0.0	0.0	0.001074817561932698	0.0
18	1.465660311726406E-4	0.0	0.0	0.0013190942805537656	0.0
19	1.465660311726406E-4	0.0	0.0	0.002222918139451716	0.0
20	1.465660311726406E-4	0.0	0.0	0.0037862891386265494	0.0
21	1.465660311726406E-4	0.0	0.0	0.00718173552745939	0.0
22	1.465660311726406E-4	0.0	0.0	0.011969559212432318	0.0
23	1.465660311726406E-4	0.0	0.0	0.015023018195195663	0.0
24	1.465660311726406E-4	0.0	0.0	0.019468854474099094	0.0
25	1.465660311726406E-4	0.0	0.0	0.02222918139451716	0.0
26	1.465660311726406E-4	0.0	0.0	0.028311671688181744	0.0
27	1.465660311726406E-4	0.0	0.0	0.07049826099404014	0.0
28	1.465660311726406E-4	0.0	0.0	0.17648992920372142	0.0
29	1.465660311726406E-4	0.0	0.0	0.35048823587750794	0.0
30	1.465660311726406E-4	0.0	0.0	0.5771037477422725	0.0
31	1.465660311726406E-4	0.0	0.0	0.8681106026355504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18825	0.0	21.698805	1
GTATTGG	1390	0.0	21.028776	1
ATTGGAC	1395	0.0	20.02509	3
TTGGACC	1890	0.0	18.989418	4
GGACCCT	1820	0.0	18.398354	6
TGGACCC	2000	0.0	17.02	5
ACCGTTA	255	0.0	16.686274	8
TATTGGA	1715	0.0	16.180758	2
TATACCG	265	0.0	16.056604	5
GACCCTC	2075	0.0	15.959036	7
GTACCGA	85	5.3683197E-4	15.235294	6
ATCCGAT	85	5.3683197E-4	15.235294	7
TATACAC	1955	0.0	15.046036	37
TAATACT	760	0.0	14.848684	4
CTAATAC	790	0.0	14.753164	3
TGTTACG	215	1.2732926E-11	14.627907	16
GATACTG	2220	0.0	14.416667	36
ACTCTGT	2060	0.0	14.099515	37
GTATCGT	105	1.6572668E-4	14.095238	6
CCGTTTA	290	0.0	14.034484	27
>>END_MODULE