FastQCFastQC Report
Wed 25 May 2016
SRR2088516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4309476
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT918132.1304910388177127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT639551.4840551380260616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT630251.4624747881180913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277040.6428623804843094No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG150180.34848784399773897No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA133690.31022333109640243No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA121600.28216887621604114No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG120040.278548946554059No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113900.26430127467933456No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA106830.24789556781381308No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA94530.21935381470972343No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC83410.19355021352944068No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT82650.19178665805309045No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT64640.14999503419905344No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA63990.14848673017322755No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58240.13514404071399863No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57430.1332644618510464No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT56820.13184897653450212No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG50880.11806539820618561No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA49720.11537365563701946No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG49000.11370291886995078No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47020.10910839276051196No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46920.10887634598730797No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT45440.10544205374388904No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT44770.10388734036342237No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44660.10363208891289799No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43510.10096355102105221No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC43380.10066189021588703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227500.020.7769221
GTATTGG14650.020.2047791
ATTGGAC15750.018.9111123
TTGGACC21400.017.203274
TATACCG3150.017.0317465
GGACCCT20600.016.8834936
CTAATAC8100.016.672843
TGGACCC22600.016.6172565
GACCCTC21600.016.0162057
CAATGCG2350.015.74467919
TATTGGA19350.015.6795862
TCGCTAA957.066881E-515.57894814
AATACTG9750.015.5589745
TATACTG3350.015.4626865
GATCGGT1201.9384097E-615.41666611
CGCTTAT1802.0190782E-1015.4166661
ACGTTTA2109.094947E-1214.9761926
GACGTAT2400.014.64583328
TTGCGAT4600.014.47826111
ATACTGG7200.014.1319456
Produced by FastQC (version 0.11.5)