##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4309476
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.721908881729473	31.0	31.0	34.0	30.0	34.0
2	31.866500474767697	31.0	31.0	34.0	30.0	34.0
3	31.975375428474365	33.0	31.0	34.0	30.0	34.0
4	35.6380675980096	37.0	35.0	37.0	33.0	37.0
5	35.57336112325489	37.0	35.0	37.0	33.0	37.0
6	35.64032958995479	37.0	35.0	37.0	33.0	37.0
7	35.48580314636861	37.0	35.0	37.0	33.0	37.0
8	35.53982177879631	37.0	35.0	37.0	33.0	37.0
9	37.121989309141064	39.0	37.0	39.0	33.0	39.0
10	36.996223902859654	39.0	37.0	39.0	33.0	39.0
11	37.06273848607116	39.0	37.0	39.0	33.0	39.0
12	36.91695950969445	39.0	37.0	39.0	33.0	39.0
13	36.9765693091225	39.0	37.0	39.0	33.0	39.0
14	37.953808537279244	40.0	37.0	41.0	33.0	41.0
15	37.966017446204596	40.0	37.0	41.0	33.0	41.0
16	37.855670155721945	40.0	37.0	41.0	32.0	41.0
17	37.93631058625225	40.0	37.0	41.0	33.0	41.0
18	37.92952228994894	40.0	37.0	41.0	33.0	41.0
19	38.00590280581676	40.0	37.0	41.0	33.0	41.0
20	37.92219657331889	40.0	37.0	41.0	32.0	41.0
21	37.79247825025595	40.0	37.0	41.0	32.0	41.0
22	37.682842647226714	40.0	37.0	41.0	32.0	41.0
23	37.52080925848061	39.0	36.0	41.0	32.0	41.0
24	37.652245887899134	40.0	37.0	41.0	32.0	41.0
25	37.59024159781839	40.0	36.0	41.0	32.0	41.0
26	37.371216825433066	39.0	36.0	41.0	32.0	41.0
27	37.31943396366519	39.0	36.0	41.0	32.0	41.0
28	37.15735161304994	39.0	36.0	41.0	31.0	41.0
29	36.933037566516205	39.0	35.0	41.0	31.0	41.0
30	36.76919305270525	39.0	35.0	41.0	30.0	41.0
31	36.47420962548579	38.0	35.0	40.0	30.0	41.0
32	36.30296073118866	38.0	35.0	40.0	30.0	41.0
33	36.113152503923914	38.0	35.0	40.0	30.0	41.0
34	35.91268010310302	38.0	35.0	40.0	29.0	41.0
35	35.698646888856096	38.0	35.0	40.0	28.0	41.0
36	35.587503677941356	38.0	35.0	40.0	27.0	41.0
37	35.40845383522266	38.0	35.0	40.0	26.0	41.0
38	35.242649686411994	38.0	34.0	40.0	25.0	41.0
39	35.08479708437871	38.0	34.0	40.0	24.0	41.0
40	34.858800002598926	38.0	34.0	40.0	24.0	41.0
41	34.682970040905204	38.0	34.0	40.0	23.0	41.0
42	34.340863715217345	38.0	33.0	40.0	21.0	41.0
43	33.91539272988177	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	6.0
13	10.0
14	14.0
15	25.0
16	49.0
17	118.0
18	304.0
19	629.0
20	1419.0
21	2918.0
22	5502.0
23	9400.0
24	15423.0
25	24063.0
26	35636.0
27	50200.0
28	68669.0
29	90061.0
30	112772.0
31	137415.0
32	168682.0
33	206942.0
34	257092.0
35	318701.0
36	422195.0
37	631868.0
38	788681.0
39	960678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85441571086601	21.18858534076997	15.158362640840789	21.798636307523232
2	18.417575593877306	24.63471196962229	35.47345431323901	21.47425812326139
3	19.523649743031406	24.40614589801637	34.2220956793819	21.848108679570323
4	12.910595162845784	16.246963667972626	36.398207113811516	34.444234055370075
5	11.809208358510407	39.70480401793628	34.593184879089705	13.892802744463594
6	31.435028295783525	38.25743547475378	13.158931619528685	17.148604609934015
7	24.777745600625227	34.816645921685144	20.969277935414883	19.436330542274746
8	27.947550931946253	34.01717517396546	18.58318737591299	19.452086518175296
9	24.39073799227563	14.48408112726466	22.37545817635369	38.74972270410602
10	16.409117953087566	28.741823831946157	32.363447435372656	22.48561077959362
11	32.572173507869636	23.19277332093275	23.516757025680153	20.71829614551746
12	21.614553602340518	28.655711274410162	30.590888544222082	19.13884657902724
13	32.46582647171025	21.239658835552163	26.27618763858994	20.01832705414765
14	20.046938421283702	23.73402241943104	29.48513926055047	26.733899898734787
15	22.049873348871184	30.750281472735896	26.257786329474857	20.94205884891806
16	20.58001947336521	28.781480625486715	27.331629181830923	23.306870719317153
17	21.00575568816255	27.386415424984385	27.822988224090356	23.78484066276271
18	22.434096395942337	25.48674131147267	31.05832356416418	21.02083872842081
19	22.125102912743916	24.843600474860516	32.944910239667195	20.08638637272838
20	22.91276247970751	24.63682359525845	32.14859532806308	20.301818596970953
21	21.49516553752707	25.584665049764748	33.10369984657067	19.816469566137506
22	20.043248877589757	26.257066984477927	32.49856827140933	21.201115866522983
23	21.3408312286691	26.15758853280538	33.04517765036863	19.45640258815689
24	20.83761459629895	26.378241809445047	31.061038511410665	21.72310508284534
25	20.818911626378707	26.566803017350598	32.36541983294489	20.248865523325808
26	20.207978881887264	27.82094621248616	32.157830789636606	19.813244115989974
27	19.141909596433536	27.573537942896074	31.939103501214532	21.345448959455858
28	19.87656968039734	27.307078633225938	31.790616771041307	21.025734915335413
29	20.030184644258373	27.180218662315326	30.997875379744542	21.791721313681755
30	20.060814818321298	27.60908750855092	32.96229054298016	19.36780713014761
31	21.116465203658173	27.44702604214526	32.41199162032692	19.02451713386964
32	20.315161286430182	27.303017814694876	31.38919905807574	20.992621840799206
33	19.89527265031758	27.11269305131297	32.19089745481817	20.801136843551284
34	19.05366220858406	27.666310242823027	32.96393807506991	20.316089473523
35	18.5804724286665	29.9507875203389	31.566181131998416	19.902558918996185
36	21.1205956362212	27.610688631286028	31.260737964430014	20.007977768062755
37	20.05308766077361	28.50246758538625	31.21210096076646	20.23234379307368
38	19.70536557112744	26.837415964261087	32.622481248300254	20.83473721631122
39	19.08691451118419	26.703246519994543	33.39958268708307	20.810256281738198
40	17.978079005428967	27.049413896260244	33.21308669545903	21.759420402851763
41	19.32241878130891	26.06439854868666	33.423042615853994	21.190140054150437
42	18.3381691880869	27.968388732179967	32.48497033049958	21.20847174923355
43	18.342392439359216	27.802452084661798	31.96288829546794	21.89226718051104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1530.0
1	1357.0
2	1184.0
3	3270.0
4	5356.0
5	5356.0
6	8217.5
7	11079.0
8	12009.5
9	12940.0
10	19502.5
11	26065.0
12	26065.0
13	48291.5
14	70518.0
15	120625.0
16	170732.0
17	152780.5
18	134829.0
19	134829.0
20	134435.0
21	134041.0
22	102899.5
23	71758.0
24	78827.5
25	85897.0
26	85897.0
27	93624.5
28	101352.0
29	106236.0
30	111120.0
31	118961.0
32	126802.0
33	126802.0
34	140709.5
35	154617.0
36	169083.5
37	183550.0
38	192312.0
39	201074.0
40	201074.0
41	207464.5
42	213855.0
43	228082.0
44	242309.0
45	260533.5
46	278758.0
47	278758.0
48	373396.5
49	468035.0
50	431153.0
51	394271.0
52	348849.5
53	303428.0
54	303428.0
55	267090.0
56	230752.0
57	195200.5
58	159649.0
59	141933.5
60	124218.0
61	124218.0
62	107980.0
63	91742.0
64	78814.5
65	65887.0
66	55975.0
67	46063.0
68	46063.0
69	39373.5
70	32684.0
71	27167.0
72	21650.0
73	17841.5
74	14033.0
75	14033.0
76	11001.5
77	7970.0
78	6229.0
79	4488.0
80	3468.0
81	2448.0
82	2448.0
83	1887.0
84	1326.0
85	1017.5
86	709.0
87	534.0
88	359.0
89	359.0
90	299.5
91	240.0
92	165.5
93	91.0
94	68.0
95	45.0
96	45.0
97	30.5
98	16.0
99	11.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4309476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.43350019591054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70627362177841	30.610696293178343
2	12.807828806484958	10.357306971123965
3	4.615453482805042	5.598568179036409
4	2.097276156492212	3.3920086353762544
5	1.1723364449367988	2.370083293801256
6	0.7117446345011668	1.7266996091124718
7	0.4687448444808893	1.3267096332805066
8	0.34710828279565287	1.1227842256336162
9	0.24408235008475587	0.8882193374973254
>10	1.5315120007014702	11.92870029752445
>50	0.1631227436081403	4.612187896006446
>100	0.11633784031473036	8.885752391723889
>500	0.01098781357198744	2.9524334126327845
>1k	0.006097948872313441	5.333495616228288
>5k	5.177503759511411E-4	1.4249156452176188
>10k+	5.75278195501268E-4	7.46943856262634
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	91813	2.1304910388177127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	63955	1.4840551380260616	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	63025	1.4624747881180913	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27704	0.6428623804843094	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	15018	0.34848784399773897	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13369	0.31022333109640243	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12160	0.28216887621604114	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12004	0.278548946554059	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11390	0.26430127467933456	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10683	0.24789556781381308	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9453	0.21935381470972343	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8341	0.19355021352944068	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8265	0.19178665805309045	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	6464	0.14999503419905344	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6399	0.14848673017322755	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5824	0.13514404071399863	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5743	0.1332644618510464	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5682	0.13184897653450212	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	5088	0.11806539820618561	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4972	0.11537365563701946	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4900	0.11370291886995078	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4702	0.10910839276051196	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4692	0.10887634598730797	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	4544	0.10544205374388904	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4477	0.10388734036342237	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4466	0.10363208891289799	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4351	0.10096355102105221	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4338	0.10066189021588703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.320467732039812E-5
2	0.0	0.0	0.0	0.0	2.320467732039812E-5
3	0.0	0.0	0.0	0.0	2.320467732039812E-5
4	0.0	0.0	0.0	0.0	2.320467732039812E-5
5	0.0	0.0	0.0	0.0	4.640935464079624E-5
6	0.0	0.0	0.0	2.320467732039812E-5	4.640935464079624E-5
7	0.0	0.0	0.0	2.320467732039812E-5	4.640935464079624E-5
8	2.320467732039812E-5	0.0	0.0	6.961403196119435E-5	4.640935464079624E-5
9	4.640935464079624E-5	0.0	0.0	1.392280639223887E-4	4.640935464079624E-5
10	6.961403196119435E-5	0.0	0.0	2.3204677320398117E-4	4.640935464079624E-5
11	1.1602338660199058E-4	0.0	0.0	4.4088886908756424E-4	4.640935464079624E-5
12	1.1602338660199058E-4	0.0	0.0	6.497309649711473E-4	4.640935464079624E-5
13	1.1602338660199058E-4	0.0	0.0	6.961403196119435E-4	4.640935464079624E-5
14	1.6243274124278683E-4	0.0	0.0	7.657543515731379E-4	4.640935464079624E-5
15	1.6243274124278683E-4	0.0	0.0	0.0010906198340587116	4.640935464079624E-5
16	1.6243274124278683E-4	0.0	0.0	0.0017403507990298589	4.640935464079624E-5
17	1.6243274124278683E-4	0.0	0.0	0.0023204677320398117	4.640935464079624E-5
18	1.8563741856318495E-4	0.0	0.0	0.002877379987729367	4.640935464079624E-5
19	2.0884209588358307E-4	0.0	0.0	0.003898385789826884	4.640935464079624E-5
20	2.0884209588358307E-4	0.0	0.0	0.006682947068274658	4.640935464079624E-5
21	2.0884209588358307E-4	0.0	0.0	0.012182455593209011	4.640935464079624E-5
22	2.0884209588358307E-4	0.0	0.0	0.02046652539659114	4.640935464079624E-5
23	2.0884209588358307E-4	0.0	0.0	0.02503784682870957	4.640935464079624E-5
24	2.0884209588358307E-4	0.0	0.0	0.031929635992867814	4.640935464079624E-5
25	2.0884209588358307E-4	0.0	0.0	0.03599045452393748	4.640935464079624E-5
26	2.0884209588358307E-4	0.0	0.0	0.04369440739430966	4.640935464079624E-5
27	2.0884209588358307E-4	0.0	0.0	0.09432701330741836	4.640935464079624E-5
28	2.0884209588358307E-4	0.0	0.0	0.22585112435943488	4.640935464079624E-5
29	2.0884209588358307E-4	0.0	0.0	0.4290312789768408	4.640935464079624E-5
30	2.0884209588358307E-4	0.0	0.0	0.7037282490957137	4.640935464079624E-5
31	2.0884209588358307E-4	0.0	0.0	1.0433983157117013	4.640935464079624E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22750	0.0	20.776922	1
GTATTGG	1465	0.0	20.204779	1
ATTGGAC	1575	0.0	18.911112	3
TTGGACC	2140	0.0	17.20327	4
TATACCG	315	0.0	17.031746	5
GGACCCT	2060	0.0	16.883493	6
CTAATAC	810	0.0	16.67284	3
TGGACCC	2260	0.0	16.617256	5
GACCCTC	2160	0.0	16.016205	7
CAATGCG	235	0.0	15.744679	19
TATTGGA	1935	0.0	15.679586	2
TCGCTAA	95	7.066881E-5	15.578948	14
AATACTG	975	0.0	15.558974	5
TATACTG	335	0.0	15.462686	5
GATCGGT	120	1.9384097E-6	15.416666	11
CGCTTAT	180	2.0190782E-10	15.416666	1
ACGTTTA	210	9.094947E-12	14.97619	26
GACGTAT	240	0.0	14.645833	28
TTGCGAT	460	0.0	14.478261	11
ATACTGG	720	0.0	14.131945	6
>>END_MODULE