FastQCFastQC Report
Wed 25 May 2016
SRR2088515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888301
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176781.9900911965651282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124941.4065052273947682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112701.2687140957851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49520.5574686958587235No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG30040.33817365960412066No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25680.28909119769087277No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA24570.2765954333047019No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23210.26128530757029433No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20370.2293141626543255No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19440.21884473843888502No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18810.21175254784132855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.18000655183321868No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC14650.16492157500667No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT12880.14499589666115426No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11900.13396360017606646No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT10570.11899119780344725No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10300.11595168754735163No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA9390.10570741223977008No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.104243944338687No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8990.10120443408259136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA451.07949745E-732.8888926
GTAGCAC405.9386344E-527.753
GGTATCA40000.023.1251
TCACGTT759.261885E-619.73333424
GTATTGG2650.019.547171
ATACTGG1409.458745E-1118.56
CGAATTA609.234565E-418.515
TTCTGCG701.2188018E-418.57
GCCCTAA1201.0403892E-716.95833215
TATTGGA2900.016.5862062
ATTAGAC803.3814838E-416.18753
GACCGTG803.3814838E-416.18757
TCTAATA1606.2937033E-1016.18752
TAAGCCG803.3814838E-416.187536
ACCGGGC700.002591872415.8571428
ATTGGAC3150.015.8571423
CCCGAAT700.002591872415.85714213
TAGACTG957.056788E-515.5789485
TGCTCGG957.056788E-515.57894810
TACTCCG1101.4510502E-515.1363645
Produced by FastQC (version 0.11.5)