##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	888301
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44332157680786	31.0	31.0	34.0	30.0	34.0
2	31.603594952611783	31.0	31.0	34.0	30.0	34.0
3	31.69601295056518	31.0	31.0	34.0	30.0	34.0
4	35.42770187132515	37.0	35.0	37.0	33.0	37.0
5	35.338082474296435	37.0	35.0	37.0	33.0	37.0
6	35.420032173778935	37.0	35.0	37.0	33.0	37.0
7	35.2560483439735	37.0	35.0	37.0	32.0	37.0
8	35.324701874702384	37.0	35.0	37.0	32.0	37.0
9	36.86190041438657	39.0	37.0	39.0	33.0	39.0
10	36.68831060642733	39.0	35.0	39.0	32.0	39.0
11	36.76812927149694	39.0	37.0	39.0	32.0	39.0
12	36.58739098571318	38.0	35.0	39.0	32.0	39.0
13	36.66905699757177	39.0	35.0	39.0	32.0	39.0
14	37.57513162768026	39.0	36.0	41.0	32.0	41.0
15	37.596536534350406	39.0	36.0	41.0	32.0	41.0
16	37.4692069467444	39.0	36.0	41.0	32.0	41.0
17	37.54017500824608	39.0	36.0	41.0	32.0	41.0
18	37.514160177687515	39.0	36.0	41.0	32.0	41.0
19	37.60155735499566	39.0	37.0	41.0	32.0	41.0
20	37.504254751486265	39.0	36.0	41.0	32.0	41.0
21	37.361932498105936	39.0	36.0	41.0	31.0	41.0
22	37.24472673114181	39.0	36.0	41.0	31.0	41.0
23	37.057365690233375	39.0	36.0	40.0	31.0	41.0
24	37.205852520710884	39.0	36.0	41.0	31.0	41.0
25	37.14319132816466	39.0	36.0	41.0	31.0	41.0
26	36.90010368107207	39.0	36.0	40.0	31.0	41.0
27	36.85780945873077	39.0	35.0	40.0	31.0	41.0
28	36.68056548399698	39.0	35.0	40.0	30.0	41.0
29	36.454109586727924	39.0	35.0	40.0	30.0	41.0
30	36.30015839225668	38.0	35.0	40.0	30.0	41.0
31	35.99109423494964	38.0	35.0	40.0	29.0	41.0
32	35.828556986877196	38.0	35.0	40.0	29.0	41.0
33	35.633757026053104	38.0	34.0	40.0	28.0	41.0
34	35.421801844194704	38.0	34.0	40.0	27.0	41.0
35	35.192435897291574	38.0	34.0	40.0	26.0	41.0
36	35.11242247841666	38.0	34.0	40.0	25.0	41.0
37	34.93634702651466	38.0	34.0	40.0	25.0	41.0
38	34.78948351966282	38.0	33.0	40.0	24.0	41.0
39	34.63493680633029	38.0	33.0	40.0	24.0	41.0
40	34.42332610230091	38.0	33.0	40.0	23.0	41.0
41	34.247375608042766	38.0	33.0	40.0	22.0	41.0
42	33.915623195290785	38.0	33.0	40.0	20.0	41.0
43	33.4891663974261	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	8.0
16	18.0
17	33.0
18	86.0
19	189.0
20	418.0
21	826.0
22	1509.0
23	2482.0
24	4007.0
25	6175.0
26	8944.0
27	12128.0
28	16456.0
29	21161.0
30	26235.0
31	32050.0
32	39109.0
33	47449.0
34	58188.0
35	71088.0
36	94176.0
37	133359.0
38	171060.0
39	141141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08798819319127	22.45421315522554	15.789580333693195	21.668218317889995
2	18.927255513615318	24.99051560225644	35.01695934148447	21.065269542643765
3	19.853292971639117	24.78191513912514	33.93748290275481	21.427308986480934
4	13.819752538835372	17.180550286445698	35.887497593721044	33.11219958099788
5	11.814126067627978	40.040144050271245	34.53964365682353	13.606086225277242
6	31.462195809753673	38.64196933246726	13.269150884666347	16.62668397311272
7	24.53402619157245	34.96067211451974	21.070560541978452	19.43474115192936
8	27.419872318054356	34.96551281603871	18.663718716966436	18.950896148940505
9	24.279495351237927	14.74455167786595	22.19979488934494	38.77615808155119
10	16.455570803139928	28.99107397154793	32.38654465096854	22.166810574343607
11	32.99275808537872	23.09712586161673	23.403103227397022	20.507012825607536
12	21.252030561712754	28.785400444218794	30.694438033954707	19.268130960113744
13	32.34444180519891	21.410085095029725	26.255739889969732	19.98973320980163
14	19.8975347320334	23.69084353164074	29.163312886060016	27.248308850265847
15	22.05367324814449	31.043869138951774	25.858352067598705	21.044105545305026
16	20.65020753100582	28.925443064907054	27.28962367485796	23.13472572922917
17	21.13675432088898	27.761873509092077	27.78461354878583	23.31675862123312
18	22.40051514070118	25.84281679295644	30.721005605082063	21.035662461260316
19	22.264074902538667	25.244483570321318	32.38147880054171	20.109962726598305
20	22.83246332042855	24.980158752494933	31.81117661693503	20.376201310141496
21	21.776402368116212	25.74116206105813	32.54122195066762	19.94121362015803
22	20.36044088659137	26.328462987208162	31.94468991929537	21.366406206905094
23	21.486635723701763	26.428091378935743	32.437315729690724	19.64795716767177
24	20.932093963645208	26.57477589240584	30.731362454843573	21.761767689105383
25	21.127410641212833	26.67868211338274	31.71109792739173	20.482809318012702
26	20.395226392855577	27.880752132441593	31.670345975069264	20.05367549963357
27	19.597411237857436	27.51612347616405	31.41288819893257	21.473577087045946
28	20.272970535888174	27.410978936193924	31.299075426009875	21.016975101908024
29	20.344342739679455	27.159600180569427	30.53525775609844	21.96079932365268
30	20.4507255986428	27.518937837512286	32.40568230813655	19.624654255708368
31	21.368319972621894	27.1961868780965	31.895720031836056	19.53977311744555
32	20.567577881821588	27.162977414187306	31.08630970808318	21.183134995907917
33	20.215219841022357	27.064249618091164	31.70918416167493	21.011346379211552
34	19.407948431894145	27.45814763239037	32.50835021012022	20.625553725595267
35	18.972060146279247	29.374840284993486	31.36549435382826	20.287605214899003
36	21.37113433397013	27.16590434998947	31.0653708596523	20.397590456388095
37	20.377327054680787	27.978691907360233	30.94919402319709	20.694787014761886
38	20.092963984054954	26.286134992530684	32.43168700699425	21.18921401642011
39	19.45556742590631	26.281069142103856	33.20991420700866	21.053449224981172
40	18.2735356596469	26.762437507106263	33.0729110965765	21.89111573667034
41	19.4899026343548	25.84484313312717	33.17895623217806	21.486298000339975
42	18.67745280034583	27.740822086207267	32.16544842345106	21.416276689995847
43	18.60945783017243	27.632300312619257	31.66032684866954	22.09791500853877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	371.0
1	309.5
2	248.0
3	502.5
4	757.0
5	757.0
6	1328.0
7	1899.0
8	2154.0
9	2409.0
10	3597.0
11	4785.0
12	4785.0
13	8861.0
14	12937.0
15	22807.5
16	32678.0
17	29237.5
18	25797.0
19	25797.0
20	26329.0
21	26861.0
22	21032.5
23	15204.0
24	16693.0
25	18182.0
26	18182.0
27	19491.5
28	20801.0
29	22297.5
30	23794.0
31	25692.5
32	27591.0
33	27591.0
34	30507.0
35	33423.0
36	36141.0
37	38859.0
38	40823.5
39	42788.0
40	42788.0
41	44451.0
42	46114.0
43	48730.5
44	51347.0
45	55568.0
46	59789.0
47	59789.0
48	77242.5
49	94696.0
50	86937.0
51	79178.0
52	70539.5
53	61901.0
54	61901.0
55	54461.5
56	47022.0
57	39881.0
58	32740.0
59	28999.0
60	25258.0
61	25258.0
62	22115.5
63	18973.0
64	16389.0
65	13805.0
66	11754.0
67	9703.0
68	9703.0
69	8300.5
70	6898.0
71	5729.5
72	4561.0
73	3743.5
74	2926.0
75	2926.0
76	2329.5
77	1733.0
78	1390.0
79	1047.0
80	824.5
81	602.0
82	602.0
83	456.0
84	310.0
85	235.0
86	160.0
87	123.5
88	87.0
89	87.0
90	66.0
91	45.0
92	28.0
93	11.0
94	9.5
95	8.0
96	8.0
97	4.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	888301.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.15048574301957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.92933210590508	48.53752583682703
2	8.608105882582263	9.839148650338412
3	2.4663368175460127	4.228570413859431
4	1.1260806407123047	2.5742422241007565
5	0.6487952350053872	1.8539481414157208
6	0.42796740817626233	1.4675127155672711
7	0.28302451492422537	1.132249195357138
8	0.21134379685600505	0.9662720519275793
9	0.15885312127866852	0.8170679738583635
>10	1.0151153945653657	10.781743205240051
>50	0.07538407923071949	3.0114504482620106
>100	0.040361930917750984	4.4284192993383344
>500	0.005935578063800584	2.420116897020459
>1k	0.0027699364297736058	3.25573171125123
>5k	0.0	0.0
>10k+	5.935578063800584E-4	4.686001235636213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17678	1.9900911965651282	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12494	1.4065052273947682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11270	1.2687140957851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4952	0.5574686958587235	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3004	0.33817365960412066	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2568	0.28909119769087277	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2457	0.2765954333047019	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2321	0.26128530757029433	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2037	0.2293141626543255	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1944	0.21884473843888502	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1881	0.21175254784132855	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.18000655183321868	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1465	0.16492157500667	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1288	0.14499589666115426	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1190	0.13396360017606646	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1057	0.11899119780344725	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1030	0.11595168754735163	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	939	0.10570741223977008	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	926	0.104243944338687	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	899	0.10120443408259136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.125744539294676E-4	0.0	0.0	0.0	1.125744539294676E-4
7	1.125744539294676E-4	0.0	0.0	0.0	1.125744539294676E-4
8	1.125744539294676E-4	0.0	0.0	0.0	1.125744539294676E-4
9	1.125744539294676E-4	0.0	0.0	2.251489078589352E-4	1.125744539294676E-4
10	1.125744539294676E-4	0.0	0.0	4.502978157178704E-4	1.125744539294676E-4
11	1.125744539294676E-4	0.0	0.0	9.005956314357408E-4	1.125744539294676E-4
12	1.125744539294676E-4	0.0	0.0	0.0011257445392946761	1.125744539294676E-4
13	1.125744539294676E-4	0.0	0.0	0.0011257445392946761	1.125744539294676E-4
14	1.125744539294676E-4	0.0	0.0	0.0012383189932241435	1.125744539294676E-4
15	1.125744539294676E-4	0.0	0.0	0.001688616808942014	1.125744539294676E-4
16	1.125744539294676E-4	0.0	0.0	0.0023640635325188194	1.125744539294676E-4
17	1.125744539294676E-4	0.0	0.0	0.0032646591639545605	1.125744539294676E-4
18	1.125744539294676E-4	0.0	0.0	0.0036023825257429634	1.125744539294676E-4
19	1.125744539294676E-4	0.0	0.0	0.004615552611108172	1.125744539294676E-4
20	1.125744539294676E-4	0.0	0.0	0.010019126399722616	1.125744539294676E-4
21	1.125744539294676E-4	0.0	0.0	0.016661019181561206	1.125744539294676E-4
22	1.125744539294676E-4	0.0	0.0	0.026567571127354354	1.125744539294676E-4
23	1.125744539294676E-4	0.0	0.0	0.032196293823827736	1.125744539294676E-4
24	1.125744539294676E-4	0.0	0.0	0.039963931144960996	1.125744539294676E-4
25	1.125744539294676E-4	0.0	0.0	0.0444669093021397	1.125744539294676E-4
26	1.125744539294676E-4	0.0	0.0	0.05032078090647202	1.125744539294676E-4
27	1.125744539294676E-4	0.0	0.0	0.09490026466254119	1.125744539294676E-4
28	1.125744539294676E-4	0.0	0.0	0.22458603558928786	1.125744539294676E-4
29	1.125744539294676E-4	0.0	0.0	0.42508113803766967	1.125744539294676E-4
30	1.125744539294676E-4	0.0	0.0	0.7127088678274593	1.125744539294676E-4
31	1.125744539294676E-4	0.0	0.0	1.1256319648407465	1.125744539294676E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	45	1.07949745E-7	32.88889	26
GTAGCAC	40	5.9386344E-5	27.75	3
GGTATCA	4000	0.0	23.125	1
TCACGTT	75	9.261885E-6	19.733334	24
GTATTGG	265	0.0	19.54717	1
ATACTGG	140	9.458745E-11	18.5	6
CGAATTA	60	9.234565E-4	18.5	15
TTCTGCG	70	1.2188018E-4	18.5	7
GCCCTAA	120	1.0403892E-7	16.958332	15
TATTGGA	290	0.0	16.586206	2
ATTAGAC	80	3.3814838E-4	16.1875	3
GACCGTG	80	3.3814838E-4	16.1875	7
TCTAATA	160	6.2937033E-10	16.1875	2
TAAGCCG	80	3.3814838E-4	16.1875	36
ACCGGGC	70	0.0025918724	15.857142	8
ATTGGAC	315	0.0	15.857142	3
CCCGAAT	70	0.0025918724	15.857142	13
TAGACTG	95	7.056788E-5	15.578948	5
TGCTCGG	95	7.056788E-5	15.578948	10
TACTCCG	110	1.4510502E-5	15.136364	5
>>END_MODULE