FastQCFastQC Report
Wed 25 May 2016
SRR2088514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828147
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104301.2594382398294022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76050.9183152266445451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75290.9091381119535542No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31920.38543881702161575No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG19890.2401747515839579No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA18040.21783572240194074No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG16120.19465143265627963No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG15750.1901836268198762No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA15310.18487056041982883No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15220.18378379683800097No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12120.14635082901948568No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9750.11773272136468525No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9640.11640445476467343No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.11447243061920166No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8320.10046525556453142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACCG358.867291E-426.4285725
GGTATCA22800.024.8289471
GACGTCG705.09908E-621.14285936
ACGTCGC759.260641E-619.73333437
ATTGGAC2850.018.824563
AGACCGT609.2338456E-418.56
ATACGAC500.007033262418.49999819
GTATTGG2900.017.862071
TTGGACC3750.017.7599984
CTAGACT1054.7932554E-717.6190474
CTTAGAC852.721627E-517.4117643
AAGACCG752.0663868E-417.2666665
CTAGAAC650.001579430217.0769233
TCTAGAC1452.9740477E-916.5862083
GATACGA700.002591672615.85714318
TAATGCC700.002591672615.8571434
TAGTACT1751.3096724E-1015.8571434
AGACGTC957.055866E-515.57894835
TATAGTG957.055866E-515.5789485
GGACCCT4100.015.3414646
Produced by FastQC (version 0.11.5)