FastQCFastQC Report
Wed 25 May 2016
SRR2088513_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088513_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421621
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT487491.424734066104925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT350021.0229654307125189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349911.0226439456620122No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144660.4227820673300754No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG81120.2370806117918963No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA67570.1974794987521996No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67450.1971287877880104No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG66210.19350477449138873No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA60110.1756769671451046No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57380.1676982927098004No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50520.14764931592365138No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44860.13110744877939431No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.12982150857736727No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.10080017629071132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107700.023.5673181
GTATTGG8350.017.5029951
TTGGACC13350.015.2434464
GGACCCT12550.014.8884466
TATACCG2251.8189894E-1214.85
ATTGGAC9800.014.3469383
GTATCAA177150.014.3175272
CGCACTA2101.364242E-1014.09523829
GACCCTC13250.013.6830197
TGGACCC15700.013.5509565
GAACCGA1102.4585862E-413.4545456
TACCGAC5250.013.3904777
ATTAGAC2950.013.1694923
CTAGTAC4100.013.0853663
TCAGTAC4550.013.0109883
TAGACAG5000.012.955
GTATTAG5300.012.9150931
TAGTACT5600.012.8839294
CTAATAC5900.012.8559323
TACACTA4750.012.8526325
Produced by FastQC (version 0.11.5)