##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3421621
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749978738147796	31.0	31.0	34.0	30.0	34.0
2	31.9103436645964	31.0	31.0	34.0	30.0	34.0
3	32.0422352446399	33.0	31.0	34.0	30.0	34.0
4	35.70877458374262	37.0	35.0	37.0	35.0	37.0
5	35.61637510408079	37.0	35.0	37.0	33.0	37.0
6	35.65655693602535	37.0	35.0	37.0	33.0	37.0
7	35.51634561513388	37.0	35.0	37.0	33.0	37.0
8	35.54729790353753	37.0	35.0	37.0	33.0	37.0
9	37.157131955877055	39.0	37.0	39.0	33.0	39.0
10	37.01790847086805	39.0	37.0	39.0	33.0	39.0
11	37.07995128624707	39.0	37.0	39.0	33.0	39.0
12	36.936506702524916	39.0	37.0	39.0	33.0	39.0
13	36.983691063387795	39.0	37.0	39.0	33.0	39.0
14	38.039112455762925	40.0	37.0	41.0	33.0	41.0
15	38.04234221148397	40.0	37.0	41.0	33.0	41.0
16	37.94207511585883	40.0	37.0	41.0	33.0	41.0
17	37.965857703117905	40.0	37.0	41.0	33.0	41.0
18	37.932051211984025	40.0	37.0	41.0	33.0	41.0
19	37.99633419364681	40.0	37.0	41.0	33.0	41.0
20	37.89401426984462	40.0	37.0	41.0	32.0	41.0
21	37.75504534254378	40.0	37.0	41.0	32.0	41.0
22	37.64484231304402	40.0	37.0	41.0	32.0	41.0
23	37.47439590767066	39.0	36.0	41.0	32.0	41.0
24	37.62384407858147	39.0	37.0	41.0	32.0	41.0
25	37.58089046098326	40.0	37.0	41.0	32.0	41.0
26	37.380807225581094	39.0	36.0	41.0	32.0	41.0
27	37.34221879045049	39.0	36.0	41.0	31.0	41.0
28	37.19572419037643	39.0	36.0	41.0	31.0	41.0
29	36.988841546156046	39.0	36.0	41.0	31.0	41.0
30	36.87309699116296	39.0	35.0	41.0	30.0	41.0
31	36.64438112812611	39.0	35.0	40.0	30.0	41.0
32	36.48049272552395	39.0	35.0	40.0	30.0	41.0
33	36.31804779079857	38.0	35.0	40.0	30.0	41.0
34	36.13245914728721	38.0	35.0	40.0	30.0	41.0
35	35.95421702169819	38.0	35.0	40.0	29.0	41.0
36	35.9010927861385	38.0	35.0	40.0	28.0	41.0
37	35.77506041727006	38.0	35.0	40.0	27.0	41.0
38	35.64399856091601	38.0	35.0	40.0	27.0	41.0
39	35.529039306223574	38.0	35.0	40.0	26.0	41.0
40	35.364725958836466	38.0	35.0	40.0	25.0	41.0
41	35.24639081885457	38.0	34.0	40.0	25.0	41.0
42	34.986782872796255	38.0	34.0	40.0	24.0	41.0
43	34.61164781254265	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	3.0
13	4.0
14	6.0
15	18.0
16	38.0
17	99.0
18	191.0
19	476.0
20	1022.0
21	2152.0
22	4100.0
23	7041.0
24	11375.0
25	17616.0
26	26252.0
27	37446.0
28	50748.0
29	66775.0
30	84968.0
31	104813.0
32	128624.0
33	159459.0
34	198331.0
35	247603.0
36	325729.0
37	473306.0
38	645433.0
39	827991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.046334471292994	21.41154733385141	14.513238023732026	23.02888017112357
2	18.131260008048816	23.76096008295483	35.863732423900835	22.244047485095514
3	18.841215903222476	24.66459610810198	33.28331805305147	23.21086993562408
4	13.304688041136059	16.61025578227396	36.826697053823324	33.25835912276667
5	12.30481108223266	38.88639332059278	35.01767144870808	13.791124148466471
6	31.41031692288538	38.55085060560477	14.236176362022562	15.802656109487287
7	25.335447730768546	33.97912860600283	21.792419440960877	18.893004222267752
8	26.80846300627685	34.85441549487801	19.488277632151544	18.848843866693592
9	24.65235045026904	14.735267289977468	22.080703853524398	38.53167840622909
10	16.02950765149033	28.377456182318262	33.32236387373119	22.270672292460212
11	33.198095288753485	23.142715104916647	23.59092371715044	20.068265889179425
12	20.95004093089211	27.77338577241606	31.167508032011725	20.109065264680105
13	31.22557992249872	21.59342019469719	26.225990546585958	20.955009336218126
14	20.286992627178755	22.985918078010393	28.617108674514213	28.10998062029664
15	22.46441087426106	30.41353791083232	25.371483282338986	21.750567932567634
16	21.37863895504499	28.842615824487865	26.642226009251168	23.13651921121597
17	21.337927257285365	27.642541356859805	27.595575313572134	23.4239560722827
18	22.47656885435295	26.242941576521773	29.928796906495485	21.35169266262979
19	22.481128096887414	25.718979396023112	31.145822404059366	20.654070103030115
20	23.12605633411766	25.44001804992429	30.49338310701273	20.940542508945324
21	22.009158816829803	26.142755144418388	31.060073573315105	20.788012465436704
22	21.147052815025393	26.490718872721438	30.707872087528106	21.654356224725063
23	21.848591647058512	26.476193593621268	31.052620965326085	20.622593793994135
24	21.56626932088621	26.528244945889686	29.731463537311704	22.174022195912404
25	21.581788281051583	26.637491411234613	30.673590090778614	21.10713021693519
26	21.141295310029953	27.406512877960477	30.50256004390901	20.949631768100556
27	20.35441681004413	27.423493133809966	30.320570279408503	21.9015197767374
28	20.884107269624543	27.240685043726348	30.212142139646676	21.66306554700243
29	20.908978522168294	27.156076023615704	29.755107301480788	22.179838152735208
30	21.080505409570492	27.34771033963142	31.063376101561218	20.508408149236868
31	21.736305686690606	27.136816146498983	30.59503083480023	20.531847332010177
32	21.082814256751405	27.16908155520439	30.030707667506135	21.717396520538074
33	20.96731344587843	26.761789222126005	30.69328835660057	21.577608975394995
34	20.259111105525715	27.065358787545435	31.310159716695686	21.365370390233167
35	20.004319590042265	28.45005335190543	30.508200645249723	21.03742641280259
36	21.585120035211382	26.8484732821081	30.296458900620493	21.269947782060022
37	20.791168864114407	27.26201996071453	30.36619193066678	21.580619244504287
38	20.448787285324705	26.09292496158984	31.54364554110464	21.914642211980812
39	20.06350206524919	25.903599492755042	32.249831293413266	21.7830671485825
40	19.223052465483466	26.03885702127734	32.253952147242494	22.4841383659967
41	19.959136327489222	25.329076481585776	32.47259120750077	22.23919598342423
42	19.145399212829243	26.745130451327014	31.850809893907012	22.25866044193673
43	19.058802830588192	26.638338962731407	31.473854059230987	22.82900414744941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1624.0
1	1490.5
2	1357.0
3	2748.5
4	4140.0
5	4140.0
6	5820.0
7	7500.0
8	7997.5
9	8495.0
10	12325.0
11	16155.0
12	16155.0
13	27941.5
14	39728.0
15	66303.5
16	92879.0
17	86560.5
18	80242.0
19	80242.0
20	81345.0
21	82448.0
22	67834.0
23	53220.0
24	59884.0
25	66548.0
26	66548.0
27	74073.0
28	81598.0
29	88665.0
30	95732.0
31	104770.5
32	113809.0
33	113809.0
34	126132.0
35	138455.0
36	150026.5
37	161598.0
38	171358.0
39	181118.0
40	181118.0
41	187594.5
42	194071.0
43	202919.5
44	211768.0
45	222209.0
46	232650.0
47	232650.0
48	283052.5
49	333455.0
50	308639.0
51	283823.0
52	256549.5
53	229276.0
54	229276.0
55	207422.0
56	185568.0
57	163092.5
58	140617.0
59	125831.0
60	111045.0
61	111045.0
62	97141.5
63	83238.0
64	72532.5
65	61827.0
66	52678.0
67	43529.0
68	43529.0
69	37337.0
70	31145.0
71	26375.0
72	21605.0
73	17558.5
74	13512.0
75	13512.0
76	10884.5
77	8257.0
78	6410.0
79	4563.0
80	3558.5
81	2554.0
82	2554.0
83	1897.5
84	1241.0
85	918.0
86	595.0
87	460.5
88	326.0
89	326.0
90	257.0
91	188.0
92	131.0
93	74.0
94	55.5
95	37.0
96	37.0
97	22.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3421621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.71677028441432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.5516029973614	39.33166834405275
2	12.480305987271702	12.659216236713188
3	4.229864517907698	6.435752012667588
4	1.8971996221742238	3.8487934968595097
5	1.0157422055463083	2.5757582053438233
6	0.5936016491010123	1.8063335086743335
7	0.3930506280680172	1.3953980889709279
8	0.29226233991856754	1.1858081565148322
9	0.19891644592271016	0.9079559724288792
>10	1.1790423090375417	11.057680677456752
>50	0.10186729182125567	3.5754462527433715
>100	0.05893845643448572	5.540146883415032
>500	0.0037451569201340127	1.3134653607250937
>1k	0.0032265967289206846	3.157950843811978
>5k	4.033245911150856E-4	1.3160368860524507
>10k+	2.3047119492290605E-4	3.8925890735694746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48749	1.424734066104925	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35002	1.0229654307125189	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34991	1.0226439456620122	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14466	0.4227820673300754	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8112	0.2370806117918963	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6757	0.1974794987521996	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6745	0.1971287877880104	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6621	0.19350477449138873	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6011	0.1756769671451046	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5738	0.1676982927098004	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5052	0.14764931592365138	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4486	0.13110744877939431	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4442	0.12982150857736727	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3449	0.10080017629071132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.9225913682432975E-5	0.0	0.0	0.0
5	0.0	2.9225913682432975E-5	0.0	0.0	0.0
6	0.0	2.9225913682432975E-5	0.0	0.0	0.0
7	2.9225913682432975E-5	5.845182736486595E-5	0.0	2.9225913682432975E-5	0.0
8	5.845182736486595E-5	5.845182736486595E-5	0.0	2.9225913682432975E-5	0.0
9	5.845182736486595E-5	5.845182736486595E-5	0.0	1.4612956841216488E-4	0.0
10	5.845182736486595E-5	5.845182736486595E-5	0.0	2.338073094594638E-4	0.0
11	5.845182736486595E-5	5.845182736486595E-5	0.0	3.214850505067627E-4	0.0
12	5.845182736486595E-5	5.845182736486595E-5	0.0	5.845182736486595E-4	0.0
13	8.767774104729893E-5	8.767774104729893E-5	0.0	5.845182736486595E-4	0.0
14	8.767774104729893E-5	8.767774104729893E-5	0.0	7.014219283783914E-4	5.845182736486595E-5
15	8.767774104729893E-5	8.767774104729893E-5	0.0	9.644551515202882E-4	5.845182736486595E-5
16	1.169036547297319E-4	8.767774104729893E-5	0.0	0.0015197475114865147	5.845182736486595E-5
17	1.7535548209459786E-4	8.767774104729893E-5	0.0	0.00216271761250004	5.845182736486595E-5
18	1.7535548209459786E-4	8.767774104729893E-5	0.0	0.0026595581451014007	5.845182736486595E-5
19	1.7535548209459786E-4	8.767774104729893E-5	0.0	0.0037701428650338538	5.845182736486595E-5
20	1.7535548209459786E-4	1.4612956841216488E-4	0.0	0.0065173787511825535	5.845182736486595E-5
21	1.7535548209459786E-4	1.7535548209459786E-4	0.0	0.0109012658035475	5.845182736486595E-5
22	1.7535548209459786E-4	2.0458139577703082E-4	0.0	0.01680490036739896	5.845182736486595E-5
23	1.7535548209459786E-4	2.338073094594638E-4	0.0	0.02039968775033822	5.845182736486595E-5
24	1.7535548209459786E-4	2.338073094594638E-4	0.0	0.02583570769527075	5.845182736486595E-5
25	1.7535548209459786E-4	2.9225913682432976E-4	0.0	0.029635076473987037	5.845182736486595E-5
26	1.7535548209459786E-4	3.214850505067627E-4	0.0	0.03670774758513582	5.845182736486595E-5
27	2.0458139577703082E-4	3.507109641891957E-4	0.0	0.08630412310422457	5.845182736486595E-5
28	2.0458139577703082E-4	3.507109641891957E-4	0.0	0.2166809240415581	5.845182736486595E-5
29	2.0458139577703082E-4	3.507109641891957E-4	0.0	0.40422361214173047	5.845182736486595E-5
30	2.338073094594638E-4	3.799368778716287E-4	0.0	0.6604179714819379	5.845182736486595E-5
31	2.338073094594638E-4	3.799368778716287E-4	0.0	1.067242689941405	5.845182736486595E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10770	0.0	23.567318	1
GTATTGG	835	0.0	17.502995	1
TTGGACC	1335	0.0	15.243446	4
GGACCCT	1255	0.0	14.888446	6
TATACCG	225	1.8189894E-12	14.8	5
ATTGGAC	980	0.0	14.346938	3
GTATCAA	17715	0.0	14.317527	2
CGCACTA	210	1.364242E-10	14.095238	29
GACCCTC	1325	0.0	13.683019	7
TGGACCC	1570	0.0	13.550956	5
GAACCGA	110	2.4585862E-4	13.454545	6
TACCGAC	525	0.0	13.390477	7
ATTAGAC	295	0.0	13.169492	3
CTAGTAC	410	0.0	13.085366	3
TCAGTAC	455	0.0	13.010988	3
TAGACAG	500	0.0	12.95	5
GTATTAG	530	0.0	12.915093	1
TAGTACT	560	0.0	12.883929	4
CTAATAC	590	0.0	12.855932	3
TACACTA	475	0.0	12.852632	5
>>END_MODULE