FastQCFastQC Report
Wed 25 May 2016
SRR2088512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893254
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT554721.9172875938303378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT407951.410004099190738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT368711.2743782606020764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157810.545441222927541No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG99500.3439034388270093No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86310.29831463120762985No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79440.27456974050670974No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74320.2568734027499832No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA74250.2566314606322155No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA60250.20824303707866645No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG60220.2081393475996231No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC50460.1744057037508632No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.1690829771599728No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT46580.16099519779459392No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT39710.13725030709367375No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37310.12895514877020822No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG34330.1186553271852385No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA33550.11595940073011218No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33450.11561376913330113No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.1149570690993601No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA33190.11471512698159236No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG32150.11112055837475728No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31570.1091158951132531No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT31450.10870113719707983No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT30990.10711123185174892No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30440.10521025806928808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109000.025.71331
GTATTGG6300.021.1428591
ATTGGAC6450.019.2170543
TTGGACC13350.017.7378274
GGACCCT13900.017.3021586
GACCCTC13500.016.7185177
TGGACCC14450.016.6435995
TATTGGA8450.015.9822492
TATACCG2800.015.8571425
CCGTATA1455.3543772E-815.3103452
GTATCAA183700.015.2572142
CCTCGCA14950.015.0969910
CCCTCGC15250.015.0426239
TAAGACG750.004106407514.84
CCCGTAT1252.9623207E-614.7999991
TATACTG4650.014.7204295
ACCCTCG15400.014.6558448
ACGTTTA2405.456968E-1213.87500126
TCGCATG16650.013.55555512
CTCGCAT16650.013.44444511
Produced by FastQC (version 0.11.5)