##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2893254
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49187454679057	31.0	31.0	34.0	30.0	34.0
2	31.658772440995502	31.0	31.0	34.0	30.0	34.0
3	31.76090657785317	31.0	31.0	34.0	30.0	34.0
4	35.48379437132032	37.0	35.0	37.0	33.0	37.0
5	35.390545731553466	37.0	35.0	37.0	33.0	37.0
6	35.45910072188615	37.0	35.0	37.0	33.0	37.0
7	35.29784526349916	37.0	35.0	37.0	32.0	37.0
8	35.36048027584167	37.0	35.0	37.0	33.0	37.0
9	36.89949033164734	39.0	37.0	39.0	33.0	39.0
10	36.7389340859807	39.0	37.0	39.0	32.0	39.0
11	36.81514308802476	39.0	37.0	39.0	32.0	39.0
12	36.642648035741075	39.0	35.0	39.0	32.0	39.0
13	36.71712127590595	39.0	35.0	39.0	32.0	39.0
14	37.64706175123235	39.0	37.0	41.0	32.0	41.0
15	37.67253549118052	39.0	37.0	41.0	32.0	41.0
16	37.54857886656339	39.0	36.0	41.0	32.0	41.0
17	37.61999084767532	39.0	37.0	41.0	32.0	41.0
18	37.59025927208603	39.0	36.0	41.0	32.0	41.0
19	37.679034402095354	40.0	37.0	41.0	32.0	41.0
20	37.57947764005511	39.0	37.0	41.0	32.0	41.0
21	37.450629982711504	39.0	36.0	41.0	32.0	41.0
22	37.336385260333174	39.0	36.0	41.0	31.0	41.0
23	37.15666166883378	39.0	36.0	41.0	31.0	41.0
24	37.28853913275502	39.0	36.0	41.0	31.0	41.0
25	37.231978595726474	39.0	36.0	41.0	31.0	41.0
26	36.99917221232564	39.0	36.0	41.0	31.0	41.0
27	36.953852651720176	39.0	36.0	41.0	31.0	41.0
28	36.7849103466201	39.0	35.0	40.0	30.0	41.0
29	36.564694631027905	39.0	35.0	40.0	30.0	41.0
30	36.41371410875091	38.0	35.0	40.0	30.0	41.0
31	36.1180964408932	38.0	35.0	40.0	30.0	41.0
32	35.957150322785346	38.0	35.0	40.0	30.0	41.0
33	35.77179051683675	38.0	35.0	40.0	29.0	41.0
34	35.5657028383958	38.0	34.0	40.0	27.0	41.0
35	35.358283095780735	38.0	34.0	40.0	27.0	41.0
36	35.27900557642018	38.0	34.0	40.0	26.0	41.0
37	35.121265537004355	38.0	34.0	40.0	25.0	41.0
38	34.964413425160735	38.0	34.0	40.0	24.0	41.0
39	34.81912510965162	38.0	34.0	40.0	24.0	41.0
40	34.60469837767441	38.0	33.0	40.0	23.0	41.0
41	34.44802184668197	38.0	33.0	40.0	22.0	41.0
42	34.130487333638875	38.0	33.0	40.0	21.0	41.0
43	33.72365682377005	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	7.0
14	9.0
15	19.0
16	37.0
17	100.0
18	251.0
19	536.0
20	1281.0
21	2447.0
22	4611.0
23	7730.0
24	12543.0
25	18835.0
26	27897.0
27	38633.0
28	51959.0
29	67127.0
30	83550.0
31	101636.0
32	122806.0
33	150444.0
34	183237.0
35	226397.0
36	298842.0
37	426092.0
38	554375.0
39	511851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.567490445014506	22.805014699711812	15.972154536034514	21.65534031923917
2	18.683012276143053	25.067380879798318	35.3409344634104	20.908672380648223
3	19.244352552523907	24.86408037455405	34.56862066033608	21.322946412585967
4	13.672425580332733	17.339784201456215	36.2278251408276	32.759965077383455
5	11.469093276981559	40.12489052119171	34.871082870705436	13.534933331121291
6	30.86901461123012	39.139010954447826	13.567422701221531	16.424551733100515
7	24.025301615412957	35.45129463227217	21.28620577384495	19.237197978469915
8	27.118911785830075	34.99564849819615	19.031685431006057	18.85375428496772
9	24.10877164604283	14.74447110416161	22.858207402461037	38.28854984733452
10	16.142827418539817	29.34602354304185	32.71285549073811	21.798293547680224
11	32.82238614376753	23.360168170509745	23.690591977061125	20.126853708661596
12	20.975102773555314	28.829442558448033	31.041934099114698	19.15352056888196
13	32.08667472679551	21.963470887796234	26.472131378717528	19.477723006690738
14	19.74714283640496	23.973318623252574	29.32138001018922	26.95815853015325
15	21.609751511619788	31.558134888952022	25.8822073692804	20.949906230147786
16	20.357942994289473	29.48914958728131	27.385428310131083	22.76747910829813
17	20.853371325158456	28.099848820739552	27.87332187218958	23.173457981912406
18	22.211012237432318	26.22047701307939	31.032705735479844	20.53580501400845
19	21.816819401269296	25.7109814762202	32.65351054556565	19.81868857694485
20	22.581114551297603	25.2510840735034	32.019795012812565	20.148006362386432
21	21.55047569276669	25.901044291306608	32.939140497170314	19.609339518756393
22	20.040376683139467	26.572502794431465	32.29239465321745	21.094725869211622
23	21.243451145319423	26.55276723025355	32.76891693574087	19.434864688686165
24	20.71950820771353	26.631121913250617	31.090944659542508	21.558425219493348
25	20.602131717436492	26.96078533028901	32.26149518846254	20.175587763811954
26	20.10666882340783	28.07167984559945	31.99197166926927	19.829679661723443
27	19.04575263699627	27.836823175566334	31.79904011192934	21.318384075508064
28	19.892826554460825	27.680701383286777	31.541544572305092	20.884927489947305
29	19.873471185039403	27.279077467792316	30.80078693401962	22.046664413148655
30	20.083338690623084	27.77046190897861	32.789931336826974	19.356268063571328
31	21.082697889642596	27.56363596144687	32.146814624640626	19.206851524269904
32	20.292238427735693	27.552091866113383	31.289544575070146	20.866125131080786
33	19.964130352882947	27.237117791939458	31.984264084660385	20.81448777051721
34	19.20916725596854	27.708248221552616	32.861269698408776	20.22131482407006
35	18.603102250960337	29.681977455142206	31.716883481367347	19.998036812530113
36	21.02501197613483	27.333549007449744	31.475356121515773	20.166082894899652
37	20.072900616399387	28.3904558673383	31.26973988457287	20.26690363168944
38	19.729411935488553	26.590510200625317	32.82134924897711	20.858728614909026
39	19.290286991740096	26.26928019454911	33.57586302481565	20.864569788895132
40	18.00519415163688	26.526741171013672	33.60880171599175	21.859262961357693
41	19.170007196049845	25.76355204209516	33.71919644801321	21.347244313841784
42	18.373464618039065	27.54645807108536	32.861096882610376	21.218980428265198
43	18.175383149906647	27.57801423587421	32.350495324641386	21.896107289577756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1608.0
1	1403.5
2	1199.0
3	2057.5
4	2916.0
5	2916.0
6	4727.5
7	6539.0
8	7212.5
9	7886.0
10	11856.0
11	15826.0
12	15826.0
13	28508.0
14	41190.0
15	72170.5
16	103151.0
17	93840.0
18	84529.0
19	84529.0
20	87306.5
21	90084.0
22	72126.5
23	54169.0
24	59494.5
25	64820.0
26	64820.0
27	69874.5
28	74929.0
29	78496.5
30	82064.0
31	89194.5
32	96325.0
33	96325.0
34	105457.0
35	114589.0
36	124247.0
37	133905.0
38	140293.0
39	146681.0
40	146681.0
41	151010.5
42	155340.0
43	162193.0
44	169046.0
45	181590.5
46	194135.0
47	194135.0
48	253510.5
49	312886.0
50	283917.0
51	254948.0
52	224290.5
53	193633.0
54	193633.0
55	168257.0
56	142881.0
57	120825.5
58	98770.0
59	86713.5
60	74657.0
61	74657.0
62	65099.5
63	55542.0
64	47892.0
65	40242.0
66	34139.0
67	28036.0
68	28036.0
69	23642.5
70	19249.0
71	16117.0
72	12985.0
73	10514.0
74	8043.0
75	8043.0
76	6332.0
77	4621.0
78	3660.5
79	2700.0
80	2099.5
81	1499.0
82	1499.0
83	1139.5
84	780.0
85	607.0
86	434.0
87	321.5
88	209.0
89	209.0
90	168.5
91	128.0
92	86.0
93	44.0
94	33.0
95	22.0
96	22.0
97	16.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2893254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.09317317136099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70276666617437	36.121766733326446
2	12.646686951215862	11.911452372752002
3	4.497258009119297	6.353704506592357
4	2.0961798240497096	3.9486303780914396
5	1.1097004694819554	2.6129658188827163
6	0.6396537044945888	1.8073993605279746
7	0.4202588662726176	1.3853926496323357
8	0.29932307324164253	1.1276858657882112
9	0.20822675781696043	0.8825452888306649
>10	1.1925303277213906	10.360556345272402
>50	0.10614539613098115	3.48724855240016
>100	0.07029773210421358	6.3203851983025165
>500	0.004933818025627562	1.5932215666599865
>1k	0.005154735250643139	4.894833582973022
>5k	5.891126000735016E-4	2.027853186850216
>10k+	2.945563000367508E-4	5.164358593117526
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	55472	1.9172875938303378	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40795	1.410004099190738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	36871	1.2743782606020764	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15781	0.545441222927541	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9950	0.3439034388270093	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8631	0.29831463120762985	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7944	0.27456974050670974	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7432	0.2568734027499832	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7425	0.2566314606322155	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6025	0.20824303707866645	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6022	0.2081393475996231	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5046	0.1744057037508632	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4892	0.1690829771599728	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4658	0.16099519779459392	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3971	0.13725030709367375	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3731	0.12895514877020822	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3433	0.1186553271852385	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3355	0.11595940073011218	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3345	0.11561376913330113	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3326	0.1149570690993601	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3319	0.11471512698159236	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	3215	0.11112055837475728	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3157	0.1091158951132531	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	3145	0.10870113719707983	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	3099	0.10711123185174892	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3044	0.10521025806928808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.4563159681106466E-5	0.0	0.0	0.0	0.0
7	3.4563159681106466E-5	0.0	0.0	0.0	0.0
8	6.912631936221293E-5	0.0	0.0	0.0	0.0
9	6.912631936221293E-5	0.0	0.0	0.0	0.0
10	1.0368947904331939E-4	0.0	0.0	1.0368947904331939E-4	0.0
11	1.0368947904331939E-4	0.0	0.0	2.0737895808663878E-4	0.0
12	1.0368947904331939E-4	0.0	0.0	4.1475791617327757E-4	0.0
13	1.0368947904331939E-4	0.0	0.0	4.49321075854384E-4	0.0
14	1.0368947904331939E-4	0.0	0.0	5.530105548977035E-4	0.0
15	1.0368947904331939E-4	0.0	0.0	8.640789920276616E-4	0.0
16	1.0368947904331939E-4	0.0	0.0	0.0013479632275631521	0.0
17	1.0368947904331939E-4	0.0	0.0	0.0017627211437364297	0.0
18	1.0368947904331939E-4	0.0	0.0	0.0019701001018230684	0.0
19	1.0368947904331939E-4	0.0	0.0	0.0026268001357640913	0.0
20	1.0368947904331939E-4	0.0	0.0	0.004493210758543841	0.0
21	1.0368947904331939E-4	0.0	0.0	0.007465642491118996	0.0
22	1.0368947904331939E-4	0.0	0.0	0.012684679602966072	0.0
23	1.0368947904331939E-4	0.0	0.0	0.015587985016179015	0.0
24	1.0368947904331939E-4	0.0	0.0	0.02011575893440396	0.0
25	1.0368947904331939E-4	0.0	0.0	0.022846248549211374	0.0
26	1.0368947904331939E-4	0.0	0.0	0.028860238333723896	0.0
27	1.0368947904331939E-4	0.0	0.0	0.08336634115082879	0.0
28	1.0368947904331939E-4	0.0	0.0	0.21256343203880476	0.0
29	1.0368947904331939E-4	0.0	0.0	0.39640487838261	0.0
30	1.0368947904331939E-4	0.0	0.0	0.6664468449710949	0.0
31	1.0368947904331939E-4	0.0	0.0	1.0335421639441267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10900	0.0	25.7133	1
GTATTGG	630	0.0	21.142859	1
ATTGGAC	645	0.0	19.217054	3
TTGGACC	1335	0.0	17.737827	4
GGACCCT	1390	0.0	17.302158	6
GACCCTC	1350	0.0	16.718517	7
TGGACCC	1445	0.0	16.643599	5
TATTGGA	845	0.0	15.982249	2
TATACCG	280	0.0	15.857142	5
CCGTATA	145	5.3543772E-8	15.310345	2
GTATCAA	18370	0.0	15.257214	2
CCTCGCA	1495	0.0	15.09699	10
CCCTCGC	1525	0.0	15.042623	9
TAAGACG	75	0.0041064075	14.8	4
CCCGTAT	125	2.9623207E-6	14.799999	1
TATACTG	465	0.0	14.720429	5
ACCCTCG	1540	0.0	14.655844	8
ACGTTTA	240	5.456968E-12	13.875001	26
TCGCATG	1665	0.0	13.555555	12
CTCGCAT	1665	0.0	13.444445	11
>>END_MODULE