FastQCFastQC Report
Wed 25 May 2016
SRR2088511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271053
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT355621.5658815536229231No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT256591.1298283219281982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT248951.0961875394365521No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105150.4630010836382947No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG61930.27269288739628705No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA50700.22324445972859286No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49590.2183568591309846No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG49020.21584701017545604No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA46550.20497099803483232No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35400.15587482986966839No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34420.1515596509636719No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.13760136817590785No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC30470.1341668380262372No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT26520.11677402508880241No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.10774737533646288No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT24000.1056778507590972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.025.05821
GTATTGG5700.018.1754381
GTATTAG2950.016.9322031
TATACCG2250.016.4444455
TCTATAC2850.015.5789473
GGACCCT7150.015.5244766
TACACCG1954.1836756E-1115.1794875
GTATCAA128500.014.8143972
ATTGGAC5250.014.8000013
ACCGCCC2059.276846E-1114.4390258
CGTATAC1803.3360266E-914.3888883
TTGGACC8250.014.3515164
ACACCGC1956.730261E-1014.230776
TAGACGT1304.448275E-614.23076816
CCCGTAT1304.448275E-614.2307681
GACCCTC7450.014.1543627
TGGACCC8000.014.1062515
AGAACCG1051.6567545E-414.0952385
CTAACGC1458.920215E-714.0344843
TATACTG3200.013.8755
Produced by FastQC (version 0.11.5)