##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2271053
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752821268371985	31.0	31.0	34.0	30.0	34.0
2	31.91626087105849	31.0	31.0	34.0	30.0	34.0
3	32.03869306440669	33.0	31.0	34.0	30.0	34.0
4	35.70745156541921	37.0	35.0	37.0	35.0	37.0
5	35.61911368867217	37.0	35.0	37.0	33.0	37.0
6	35.660608096772734	37.0	35.0	37.0	33.0	37.0
7	35.51657314910749	37.0	35.0	37.0	33.0	37.0
8	35.55550883224654	37.0	35.0	37.0	33.0	37.0
9	37.15561283686466	39.0	37.0	39.0	33.0	39.0
10	37.028922266455254	39.0	37.0	39.0	33.0	39.0
11	37.085319893459115	39.0	37.0	39.0	33.0	39.0
12	36.94058130743756	39.0	37.0	39.0	33.0	39.0
13	36.9874357841935	39.0	37.0	39.0	33.0	39.0
14	38.036359785526805	40.0	37.0	41.0	33.0	41.0
15	38.04304611120921	40.0	37.0	41.0	33.0	41.0
16	37.94158040345162	40.0	37.0	41.0	33.0	41.0
17	37.98016558838565	40.0	37.0	41.0	33.0	41.0
18	37.94311933715329	40.0	37.0	41.0	33.0	41.0
19	38.011695455808386	40.0	37.0	41.0	33.0	41.0
20	37.91320810214469	40.0	37.0	41.0	32.0	41.0
21	37.78107644339432	40.0	37.0	41.0	32.0	41.0
22	37.67602429357659	40.0	37.0	41.0	32.0	41.0
23	37.501904623097744	39.0	36.0	41.0	32.0	41.0
24	37.646039084072456	40.0	37.0	41.0	32.0	41.0
25	37.60151084100635	40.0	37.0	41.0	32.0	41.0
26	37.392245799635674	39.0	36.0	41.0	32.0	41.0
27	37.35293848272145	39.0	36.0	41.0	32.0	41.0
28	37.202364718040485	39.0	36.0	41.0	31.0	41.0
29	36.993364311621086	39.0	36.0	41.0	31.0	41.0
30	36.86202523675141	39.0	35.0	41.0	30.0	41.0
31	36.61716965654258	39.0	35.0	40.0	30.0	41.0
32	36.45755647270231	39.0	35.0	40.0	30.0	41.0
33	36.2917981218404	38.0	35.0	40.0	30.0	41.0
34	36.10321996008019	38.0	35.0	40.0	30.0	41.0
35	35.924706292631654	38.0	35.0	40.0	29.0	41.0
36	35.846529341235104	38.0	35.0	40.0	28.0	41.0
37	35.71141976871522	38.0	35.0	40.0	27.0	41.0
38	35.58095121514117	38.0	35.0	40.0	26.0	41.0
39	35.450549590872605	38.0	35.0	40.0	25.0	41.0
40	35.27449909799551	38.0	34.0	40.0	25.0	41.0
41	35.148956453239975	38.0	34.0	40.0	24.0	41.0
42	34.87768625390953	38.0	34.0	40.0	23.0	41.0
43	34.4963261535508	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	3.0
14	8.0
15	8.0
16	24.0
17	54.0
18	149.0
19	327.0
20	687.0
21	1419.0
22	2549.0
23	4680.0
24	7536.0
25	11690.0
26	17616.0
27	24904.0
28	34120.0
29	45016.0
30	56719.0
31	70151.0
32	85949.0
33	106474.0
34	132177.0
35	164664.0
36	217267.0
37	315615.0
38	421328.0
39	549914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.32283702758148	21.815739218767682	15.239714793093775	22.621708960557065
2	18.040177838209853	23.95298568549479	36.41390139287811	21.592935083417252
3	18.618984233304992	24.73249193215658	33.795732640321475	22.852791194216955
4	13.295022176937307	16.932145573000717	36.6528214004693	33.12001084959268
5	12.003726905536771	39.25800058387012	34.99957949021885	13.73869302037425
6	31.030407480582795	38.77729846022968	14.211821564710291	15.980472494477233
7	24.741914873849264	34.626008287785446	21.71450864422803	18.917568194137257
8	26.48881377933496	35.2986478078671	19.415927325342032	18.796611087455908
9	24.369576579674714	15.118053167407366	22.503481865020323	38.0088883878976
10	16.14000201668565	28.817072961309137	33.09429590590797	21.948629116097244
11	32.73027974248069	23.47237162672998	23.739032070145434	20.058316560643895
12	20.83491666640981	28.419327950514585	31.04758013133115	19.698175251744455
13	31.190069100104665	22.023572325260574	26.38009769036654	20.406260884268224
14	20.007943451782058	23.69909464904606	28.879995314948616	27.412966584223263
15	21.981389249832567	31.0144677380933	25.62357637624485	21.380566635829283
16	20.891410284128114	29.365408909435402	27.00218797183509	22.740992834601396
17	21.05609160156104	28.176709218146822	27.669543599378795	23.097655580913347
18	22.17495584647298	26.54011156939094	30.347860661992478	20.937071922143605
19	22.14360475074778	26.017534597387204	31.679753841059632	20.159106810805383
20	22.743634780870373	25.686586794759965	31.07893122705635	20.490847197313318
21	21.691920003628272	26.32668634329538	31.767950814005662	20.213442839070687
22	20.594807782997577	26.86097594375825	31.250349507475168	21.293866765769007
23	21.48721320022034	26.771766224742443	31.619869725629478	20.121150849407744
24	21.000830892101593	26.929446384562578	30.25534851014045	21.814374213195375
25	21.115359262861766	27.08699444706927	31.169241757017556	20.62840453305141
26	20.530960748163956	27.996000093348766	31.11446540437409	20.35857375411318
27	19.751278371750903	27.808642070440452	30.92627076514727	21.51380879266138
28	20.33602914595124	27.591782314195225	30.806502534286956	21.265686005566582
29	20.368348955308395	27.344716305608014	30.236150367252545	22.05078437183104
30	20.57067800707425	27.714588783264855	31.68688709598587	20.02784611367502
31	21.37810081931157	27.441015247112244	31.155987993234856	20.02489594034133
32	20.643507659222397	27.560519283345652	30.512277784798503	21.283695272633445
33	20.42607548128555	26.988317753922956	31.240838500906847	21.34476826388464
34	19.734281850753813	27.282146211471066	32.01669005522989	20.966881882545234
35	19.392986425239744	28.757409008068063	31.10063921889978	20.74896534779241
36	21.1873522986914	26.853270267140395	31.077037832230246	20.882339601937954
37	20.37151929083117	27.544315346229258	30.991482805553193	21.092682557386375
38	19.972893631280293	26.18419737452186	32.349839479747935	21.493069514449907
39	19.490121983062483	25.993008529523532	33.059290117844014	21.457579369569974
40	18.60771192922402	26.20520084736023	33.08720668341954	22.099880539996207
41	19.31161448015524	25.576990057035214	33.25268058473316	21.858714878076384
42	18.546506840659376	27.122528624387016	32.5238116415601	21.807152893393507
43	18.455932116071267	27.081534424780045	32.057816352150304	22.404717106998383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1204.0
1	1074.0
2	944.0
3	1911.5
4	2879.0
5	2879.0
6	4013.0
7	5147.0
8	5504.0
9	5861.0
10	8775.0
11	11689.0
12	11689.0
13	20359.0
14	29029.0
15	48750.5
16	68472.0
17	62824.0
18	57176.0
19	57176.0
20	58156.0
21	59136.0
22	48369.5
23	37603.0
24	42248.5
25	46894.0
26	46894.0
27	52284.0
28	57674.0
29	61703.0
30	65732.0
31	71390.0
32	77048.0
33	77048.0
34	85341.0
35	93634.0
36	101515.0
37	109396.0
38	116283.5
39	123171.0
40	123171.0
41	127224.0
42	131277.0
43	136533.5
44	141790.0
45	149595.0
46	157400.0
47	157400.0
48	194157.5
49	230915.0
50	210576.0
51	190237.0
52	170089.0
53	149941.0
54	149941.0
55	132559.0
56	115177.0
57	99625.5
58	84074.0
59	74409.5
60	64745.0
61	64745.0
62	56357.5
63	47970.0
64	41462.5
65	34955.0
66	29657.0
67	24359.0
68	24359.0
69	20673.5
70	16988.0
71	14385.5
72	11783.0
73	9505.0
74	7227.0
75	7227.0
76	5842.0
77	4457.0
78	3411.0
79	2365.0
80	1862.5
81	1360.0
82	1360.0
83	1025.0
84	690.0
85	513.0
86	336.0
87	253.5
88	171.0
89	171.0
90	132.0
91	93.0
92	64.5
93	36.0
94	22.5
95	9.0
96	9.0
97	8.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2271053.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.899880980233206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6781039640202	40.83384231483062
2	11.992226210153438	12.447902259899928
3	3.975569941388115	6.189948203599075
4	1.7530553486173137	3.639334557799992
5	0.9230829273356614	2.3953947031803042
6	0.5621935484049658	1.7506666950043621
7	0.3708985431244307	1.347471317072989
8	0.26995310896545077	1.1208427380440655
9	0.19519839852380813	0.9117696285825992
>10	1.1269743845383047	10.993212295796154
>50	0.09430547966454167	3.3832025409076727
>100	0.05034855243719608	4.913265235707509
>500	0.0037467587827908805	1.3883740364822523
>1k	0.0038319123914906583	3.916438973897306
>5k	1.7030721739958482E-4	0.49776402269296716
>10k+	3.4061443479916964E-4	4.270570476502185
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35562	1.5658815536229231	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25659	1.1298283219281982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24895	1.0961875394365521	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10515	0.4630010836382947	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6193	0.27269288739628705	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5070	0.22324445972859286	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4959	0.2183568591309846	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4902	0.21584701017545604	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4655	0.20497099803483232	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3540	0.15587482986966839	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3442	0.1515596509636719	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.13760136817590785	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3047	0.1341668380262372	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2652	0.11677402508880241	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2447	0.10774737533646288	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2400	0.1056778507590972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.2016218908145252E-4	0.0
10	0.0	0.0	0.0	3.0822706471403356E-4	0.0
11	0.0	0.0	0.0	3.9629194034661454E-4	0.0
12	0.0	0.0	0.0	5.724216916117766E-4	0.0
13	0.0	0.0	0.0	7.925838806932291E-4	0.0
14	0.0	0.0	0.0	0.001056778507590972	0.0
15	0.0	0.0	0.0	0.0013209731344887151	0.0
16	0.0	0.0	0.0	0.0017612975126516201	0.0
17	0.0	0.0	0.0	0.002509848955528559	0.0
18	0.0	0.0	0.0	0.0028180760202425924	0.0
19	0.0	0.0	0.0	0.0033464652740380785	0.0
20	4.4032437816290506E-5	0.0	0.0	0.0061205088564643805	0.0
21	4.4032437816290506E-5	0.0	0.0	0.010655849951542302	0.0
22	4.4032437816290506E-5	0.0	0.0	0.016952488559271846	0.0
23	4.4032437816290506E-5	0.0	0.0	0.021135570151819442	0.0
24	4.4032437816290506E-5	0.0	0.0	0.027344143883916403	0.0
25	4.4032437816290506E-5	0.0	0.0	0.030778674033587063	0.0
26	4.4032437816290506E-5	0.0	0.0	0.03883661015396823	0.0
27	4.4032437816290506E-5	0.0	0.0	0.09268828160329151	0.0
28	4.4032437816290506E-5	0.0	0.0	0.23363611505323742	0.0
29	4.4032437816290506E-5	0.0	0.0	0.4460926275168391	0.0
30	4.4032437816290506E-5	0.0	0.0	0.7586789035746854	0.0
31	4.4032437816290506E-5	0.0	0.0	1.24862783915655	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7560	0.0	25.0582	1
GTATTGG	570	0.0	18.175438	1
GTATTAG	295	0.0	16.932203	1
TATACCG	225	0.0	16.444445	5
TCTATAC	285	0.0	15.578947	3
GGACCCT	715	0.0	15.524476	6
TACACCG	195	4.1836756E-11	15.179487	5
GTATCAA	12850	0.0	14.814397	2
ATTGGAC	525	0.0	14.800001	3
ACCGCCC	205	9.276846E-11	14.439025	8
CGTATAC	180	3.3360266E-9	14.388888	3
TTGGACC	825	0.0	14.351516	4
ACACCGC	195	6.730261E-10	14.23077	6
TAGACGT	130	4.448275E-6	14.230768	16
CCCGTAT	130	4.448275E-6	14.230768	1
GACCCTC	745	0.0	14.154362	7
TGGACCC	800	0.0	14.106251	5
AGAACCG	105	1.6567545E-4	14.095238	5
CTAACGC	145	8.920215E-7	14.034484	3
TATACTG	320	0.0	13.875	5
>>END_MODULE