FastQCFastQC Report
Wed 25 May 2016
SRR2088510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085960
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT909892.226869572878834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT656211.606011806282979No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT645601.5800448364643802No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257070.6291544704304496No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG173180.423841643089996No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA160010.3916093158033852No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA140980.34503519368765234No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG139250.3408011825862221No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA127280.31150574161274214No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG121430.2971884208362294No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA103920.2543343547171289No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC90890.22244466416705008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78560.19226815729963093No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA76980.188401256987342No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT75060.18370223888633272No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT67040.1640740486935751No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA65310.1598400375921448No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG58430.14300188939686145No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA56610.1385476118219464No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG56010.13707916866538097No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA54850.13424017856268786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54430.13321226835309205No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53210.1302264339347424No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT52390.12821956162076964No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT51980.1272161254637833No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48530.11877257731353219No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC47380.11595806126344849No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT46950.11490567700124328No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.11414698137035115No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA44240.10827320874408951No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.10171416264476402No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT41120.10063730432994938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203550.023.7850671
GTATTGG13950.022.1469541
ATTGGAC13700.021.8759123
TTGGACC21100.019.9028434
TGGACCC20400.019.5882365
GGACCCT20450.019.268956
GACCCTC21300.017.8051647
TATTGGA18350.016.7356952
CGGTATA2109.094947E-1214.9761926
TATACAC24100.014.9688837
ATACTGT24850.014.96378337
GATACTG25650.014.78557636
ACCCTCG24800.014.7701618
TTTTTAC26550.014.7024491
CCCTCGC25050.014.6227549
TGATACT25650.014.49707735
GTATCAA334600.014.4914822
ACTCTGT22350.014.15436437
CCTCGCA26550.013.79661110
TACCGTG2750.013.4545457
Produced by FastQC (version 0.11.5)