##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088510_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4085960
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.708591616168537	31.0	31.0	34.0	30.0	34.0
2	31.854973127490236	31.0	31.0	34.0	30.0	34.0
3	31.961297467425037	33.0	31.0	34.0	30.0	34.0
4	35.635534366464675	37.0	35.0	37.0	33.0	37.0
5	35.570794623540124	37.0	35.0	37.0	33.0	37.0
6	35.6343941203536	37.0	35.0	37.0	33.0	37.0
7	35.4788810952628	37.0	35.0	37.0	33.0	37.0
8	35.533980264123976	37.0	35.0	37.0	33.0	37.0
9	37.10519755455266	39.0	37.0	39.0	33.0	39.0
10	36.99234500582482	39.0	37.0	39.0	33.0	39.0
11	37.048092247599094	39.0	37.0	39.0	33.0	39.0
12	36.89951737168254	39.0	37.0	39.0	32.0	39.0
13	36.960682923964015	39.0	37.0	39.0	33.0	39.0
14	37.93060284486388	40.0	37.0	41.0	33.0	41.0
15	37.93694015604656	40.0	37.0	41.0	33.0	41.0
16	37.8296304907537	40.0	37.0	41.0	32.0	41.0
17	37.92768309038757	40.0	37.0	41.0	33.0	41.0
18	37.915272053568806	40.0	37.0	41.0	33.0	41.0
19	38.00289944101264	40.0	37.0	41.0	33.0	41.0
20	37.91289782572517	40.0	37.0	41.0	32.0	41.0
21	37.79163354511547	40.0	37.0	41.0	32.0	41.0
22	37.69125248411634	40.0	37.0	41.0	32.0	41.0
23	37.52344858980509	39.0	36.0	41.0	32.0	41.0
24	37.6521806380875	40.0	37.0	41.0	32.0	41.0
25	37.60034018933127	40.0	36.0	41.0	32.0	41.0
26	37.381435207393125	39.0	36.0	41.0	32.0	41.0
27	37.33851383762934	39.0	36.0	41.0	32.0	41.0
28	37.181202703893334	39.0	36.0	41.0	31.0	41.0
29	36.95479128527935	39.0	35.0	41.0	31.0	41.0
30	36.79049354374492	39.0	35.0	41.0	31.0	41.0
31	36.483111190515814	38.0	35.0	40.0	30.0	41.0
32	36.32217765225308	38.0	35.0	40.0	30.0	41.0
33	36.131971433885795	38.0	35.0	40.0	30.0	41.0
34	35.937672174960106	38.0	35.0	40.0	30.0	41.0
35	35.71435133970964	38.0	35.0	40.0	28.0	41.0
36	35.59584112424008	38.0	35.0	40.0	27.0	41.0
37	35.42592805607495	38.0	35.0	40.0	26.0	41.0
38	35.26249718548395	38.0	34.0	40.0	25.0	41.0
39	35.10095742493808	38.0	34.0	40.0	25.0	41.0
40	34.86297002418036	38.0	34.0	40.0	24.0	41.0
41	34.679361765656054	38.0	34.0	40.0	23.0	41.0
42	34.33043960293297	38.0	33.0	40.0	21.0	41.0
43	33.908168215058396	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	5.0
14	12.0
15	21.0
16	48.0
17	97.0
18	233.0
19	603.0
20	1264.0
21	2640.0
22	4834.0
23	8635.0
24	14285.0
25	22159.0
26	33166.0
27	47609.0
28	64551.0
29	85735.0
30	108173.0
31	131183.0
32	160090.0
33	198648.0
34	245157.0
35	302496.0
36	401291.0
37	599031.0
38	742057.0
39	911930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.26046265749053	21.642453670618412	15.723648787555433	21.373434884335627
2	18.180329714436755	24.994811500846804	35.961756845392514	20.86310193932393
3	18.944360688797737	24.5597852157143	35.08531654739645	21.41053754809151
4	12.659130290066473	16.445315176849505	36.59350556539957	34.30204896768446
5	11.333444282371829	39.87126648327443	34.85589677823571	13.939392456118025
6	30.849592262283526	38.70123545017572	13.166012393660242	17.28315989388051
7	24.115997219747623	35.59936954840478	20.806224241059628	19.478408990787965
8	27.612580642003348	34.17507758274677	18.81315529275862	19.399186482491263
9	24.031096731245533	14.612257584508903	22.922201881565165	38.4344438026804
10	16.15926244995056	29.028110896827187	32.47165904707829	22.340967606143966
11	32.25217574327698	23.269953695092465	24.020793155096968	20.457077406533593
12	21.645170290458058	28.91753712713781	30.800962319748603	18.63633026265553
13	32.68800967214559	21.68677617989408	26.51837022388863	19.106843924071697
14	19.86916171475002	24.079702199727848	29.787100216350627	26.264035869171504
15	21.431854447914322	31.333370860214	26.48665185170682	20.748122840164857
16	20.139306307452838	29.312156751412154	27.572957150828692	22.975579790306316
17	20.766478379621926	27.687715983514277	27.75178905324575	23.79401658361805
18	22.454404839988644	25.46260854242332	31.541840840341067	20.541145777246964
19	21.937268108351525	24.889059119521484	33.38791862866988	19.78575414345711
20	22.862705459671655	24.509956044601513	32.63362832724746	19.993710168479378
21	21.47191847203595	25.347066540054236	33.824315460748515	19.356699527161304
22	19.53352455726439	26.228401648572188	33.17054009339299	21.067533700770444
23	21.225415814153834	26.05583510362314	33.797296106667716	18.921452975555315
24	20.496015624235188	26.225048703364696	31.629360052472364	21.649575619927752
25	20.474380561728456	26.600015663393673	33.039726282195616	19.88587749268226
26	19.877115781848083	27.807565419142627	32.786028252846336	19.529290546162958
27	18.42213335421786	27.694348451771432	32.624940038571104	21.258578155439604
28	19.479485849102783	27.276992432622933	32.25153941790913	20.99198230036515
29	19.5223888633271	27.13922799048449	31.267193021958118	22.07119012423029
30	19.73345309303077	27.582110446504615	33.6694926039413	19.014943856523313
31	20.979990014586537	27.44708709825843	32.91510440630843	18.657818480846604
32	20.07307952109174	27.291897130662075	31.88731656697569	20.747706781270498
33	19.574836758069093	27.07520386885824	32.809621239561814	20.54033813351085
34	18.567337908349568	27.879176497077797	33.57477802034283	19.9787075742298
35	17.86079648356812	30.52633897541826	32.13261999627994	19.480244544733676
36	20.9845666624245	27.832553426856848	31.641474708514032	19.541405202204622
37	19.790232895084632	29.12473445652919	31.440028781485868	19.64500386690031
38	19.400899666173924	27.03587406631489	33.12237515785764	20.440851109653547
39	18.94675914595346	26.74402098894752	33.881095262802376	20.428124602296645
40	17.46813478350253	27.08954566368736	33.836258798421916	21.6060607543882
41	18.941081165748074	26.239537342509472	34.06044112032423	20.758940371418223
42	18.053407277604283	28.2433014518008	33.051865412289885	20.651425858305025
43	17.90754192405212	28.331848574141695	32.285754143457105	21.474855358349078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1823.0
1	1694.5
2	1566.0
3	3441.0
4	5316.0
5	5316.0
6	8157.0
7	10998.0
8	11899.5
9	12801.0
10	18940.5
11	25080.0
12	25080.0
13	45479.0
14	65878.0
15	115772.5
16	165667.0
17	150706.0
18	135745.0
19	135745.0
20	135560.5
21	135376.0
22	104460.5
23	73545.0
24	80473.5
25	87402.0
26	87402.0
27	95723.5
28	104045.0
29	108537.5
30	113030.0
31	120083.0
32	127136.0
33	127136.0
34	138188.0
35	149240.0
36	163376.0
37	177512.0
38	185438.5
39	193365.0
40	193365.0
41	198204.0
42	203043.0
43	211915.0
44	220787.0
45	240929.5
46	261072.0
47	261072.0
48	363869.5
49	466667.0
50	423753.0
51	380839.0
52	333103.0
53	285367.0
54	285367.0
55	246362.0
56	207357.0
57	171918.5
58	136480.0
59	119818.0
60	103156.0
61	103156.0
62	89569.5
63	75983.0
64	65484.5
65	54986.0
66	46253.5
67	37521.0
68	37521.0
69	31604.5
70	25688.0
71	21316.0
72	16944.0
73	13791.0
74	10638.0
75	10638.0
76	8514.0
77	6390.0
78	4913.0
79	3436.0
80	2713.0
81	1990.0
82	1990.0
83	1519.5
84	1049.0
85	803.5
86	558.0
87	406.5
88	255.0
89	255.0
90	196.0
91	137.0
92	97.5
93	58.0
94	40.5
95	23.0
96	23.0
97	15.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4085960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.60237096429899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42686399123174	30.96334005472649
2	13.247512666318173	11.022558725968365
3	5.08595207179748	6.347629943926912
4	2.345726401264202	3.9035111970457352
5	1.3122004230319508	2.729532438924264
6	0.7976906849006307	1.991149427280106
7	0.5287672701576993	1.5398580488816216
8	0.37777992865674187	1.2573232587875345
9	0.2650407033679124	0.9923689495935558
>10	1.3836931771940368	10.736717318398755
>50	0.12804074816656355	3.7240206292617075
>100	0.08586416632419462	6.7636518208340535
>500	0.007610881033265763	2.169926613213084
>1k	0.005781909613405228	5.238838946829323
>5k	8.259870876293183E-4	2.1888017273564446
>10k+	6.48989854565893E-4	8.430770898972204
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	90989	2.226869572878834	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	65621	1.606011806282979	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	64560	1.5800448364643802	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25707	0.6291544704304496	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	17318	0.423841643089996	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	16001	0.3916093158033852	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14098	0.34503519368765234	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13925	0.3408011825862221	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	12728	0.31150574161274214	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12143	0.2971884208362294	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10392	0.2543343547171289	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	9089	0.22244466416705008	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7856	0.19226815729963093	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7698	0.188401256987342	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	7506	0.18370223888633272	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	6704	0.1640740486935751	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6531	0.1598400375921448	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	5843	0.14300188939686145	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	5661	0.1385476118219464	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	5601	0.13707916866538097	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5485	0.13424017856268786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5443	0.13321226835309205	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5321	0.1302264339347424	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	5239	0.12821956162076964	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	5198	0.1272161254637833	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4853	0.11877257731353219	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4738	0.11595806126344849	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	4695	0.11490567700124328	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4664	0.11414698137035115	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	4424	0.10827320874408951	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4156	0.10171416264476402	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4112	0.10063730432994938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	2.447405260942349E-5	0.0	0.0	0.0	0.0
5	2.447405260942349E-5	0.0	0.0	0.0	0.0
6	1.7131836826596444E-4	0.0	0.0	0.0	0.0
7	1.7131836826596444E-4	0.0	0.0	0.0	0.0
8	2.202664734848114E-4	0.0	0.0	0.0	0.0
9	2.202664734848114E-4	0.0	0.0	9.789621043769396E-5	0.0
10	2.202664734848114E-4	0.0	0.0	1.2237026304711744E-4	0.0
11	2.202664734848114E-4	0.0	0.0	4.160588943601993E-4	0.0
12	2.202664734848114E-4	0.0	0.0	5.139551047978933E-4	0.0
13	3.1816268392250536E-4	0.0	0.0	5.384291574073168E-4	0.0
14	3.1816268392250536E-4	0.0	0.0	6.852734730638577E-4	0.0
15	3.1816268392250536E-4	0.0	0.0	8.565918413298221E-4	0.0
16	3.426367365319289E-4	0.0	0.0	0.0012726507356900214	0.0
17	3.9158484175077585E-4	0.0	0.0	0.002153716629629267	0.0
18	4.160588943601993E-4	0.0	0.0	0.0026676717344271605	0.0
19	4.160588943601993E-4	0.0	0.0	0.0033284711548815945	0.0
20	4.160588943601993E-4	0.0	0.0	0.006191935310184143	0.0
21	4.160588943601993E-4	0.0	0.0	0.011845441462960968	0.0
22	4.160588943601993E-4	0.0	0.0	0.01886949456186551	0.0
23	4.160588943601993E-4	0.0	0.0	0.022907713242420386	0.0
24	4.405329469696228E-4	0.0	4.894810521884698E-5	0.02931991502608934	0.0
25	4.405329469696228E-4	0.0	4.894810521884698E-5	0.03279523049662748	0.0
26	4.894810521884697E-4	0.0	4.894810521884698E-5	0.03888926959637393	0.0
27	4.894810521884697E-4	0.0	4.894810521884698E-5	0.08512075497557489	0.0
28	5.139551047978933E-4	0.0	4.894810521884698E-5	0.21439270085854978	0.0
29	6.363253678450107E-4	0.0	4.894810521884698E-5	0.4237926949847771	0.0
30	6.852734730638577E-4	0.0	4.894810521884698E-5	0.7105796434620015	0.0
31	6.852734730638577E-4	0.0	4.894810521884698E-5	1.0558351036231388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20355	0.0	23.785067	1
GTATTGG	1395	0.0	22.146954	1
ATTGGAC	1370	0.0	21.875912	3
TTGGACC	2110	0.0	19.902843	4
TGGACCC	2040	0.0	19.588236	5
GGACCCT	2045	0.0	19.26895	6
GACCCTC	2130	0.0	17.805164	7
TATTGGA	1835	0.0	16.735695	2
CGGTATA	210	9.094947E-12	14.97619	26
TATACAC	2410	0.0	14.96888	37
ATACTGT	2485	0.0	14.963783	37
GATACTG	2565	0.0	14.785576	36
ACCCTCG	2480	0.0	14.770161	8
TTTTTAC	2655	0.0	14.702449	1
CCCTCGC	2505	0.0	14.622754	9
TGATACT	2565	0.0	14.497077	35
GTATCAA	33460	0.0	14.491482	2
ACTCTGT	2235	0.0	14.154364	37
CCTCGCA	2655	0.0	13.796611	10
TACCGTG	275	0.0	13.454545	7
>>END_MODULE