FastQCFastQC Report
Wed 25 May 2016
SRR2088509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053969
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT998381.6491329902746446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT732041.209190202328423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT718271.1864447934900229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304040.5022159842576003No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG194090.32059959342375227No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA190100.3140088758300546No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG175870.2905036348881205No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA157480.2601268688359653No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG156490.25849157800444633No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA125890.20794622503022397No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA120740.19943940908848393No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC104940.17334082814100965No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA96910.16007680250757808No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.1477543079589605No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA79280.1309554112351748No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT69860.11539537120193381No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68680.11344623667547686No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT63230.10444387805751895No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA62130.10262688824472012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225600.023.6170221
GTATTGG18050.020.3961221
ATTGGAC19500.018.405133
TTGGACC26200.017.8645044
GGACCCT27000.016.7870376
TGGACCC29150.016.0566045
GACCCTC28850.015.6464477
GTATCAA372600.014.2895872
TATTGGA26700.014.1348312
ACGTTCG4850.014.11340222
GATACCT23700.013.97257336
CCCTCGC30950.013.9273019
ACCCTCG32350.013.8392588
TAATACT12150.013.5514414
CTAATAC9850.013.5228413
CGTTCGC5750.013.51304323
CCTCGCA32050.013.33385410
GTATTAG9600.013.2968761
ACCGTTA3350.013.2537328
CTAGTAC8300.013.1506023
Produced by FastQC (version 0.11.5)