##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6053969
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.738888983409066	31.0	31.0	34.0	30.0	34.0
2	31.89342677506277	31.0	31.0	34.0	30.0	34.0
3	32.01411586349385	33.0	31.0	34.0	30.0	34.0
4	35.68028957531828	37.0	35.0	37.0	35.0	37.0
5	35.60038579649152	37.0	35.0	37.0	33.0	37.0
6	35.649323609024094	37.0	35.0	37.0	33.0	37.0
7	35.50516479354288	37.0	35.0	37.0	33.0	37.0
8	35.54509050178486	37.0	35.0	37.0	33.0	37.0
9	37.14356234727994	39.0	37.0	39.0	33.0	39.0
10	37.00844685527792	39.0	37.0	39.0	33.0	39.0
11	37.06982840513389	39.0	37.0	39.0	33.0	39.0
12	36.923741763461294	39.0	37.0	39.0	33.0	39.0
13	36.97773345056772	39.0	37.0	39.0	33.0	39.0
14	38.004367713148184	40.0	37.0	41.0	33.0	41.0
15	38.01203854859514	40.0	37.0	41.0	33.0	41.0
16	37.90598399165903	40.0	37.0	41.0	33.0	41.0
17	37.94819679453264	40.0	37.0	41.0	33.0	41.0
18	37.92120970556671	40.0	37.0	41.0	33.0	41.0
19	37.98213832941662	40.0	37.0	41.0	33.0	41.0
20	37.88849975941403	40.0	37.0	41.0	32.0	41.0
21	37.752300020036444	40.0	37.0	41.0	32.0	41.0
22	37.64257101415617	40.0	37.0	41.0	32.0	41.0
23	37.46500370252969	39.0	36.0	41.0	32.0	41.0
24	37.61393872350519	39.0	37.0	41.0	32.0	41.0
25	37.574930099575994	40.0	37.0	41.0	32.0	41.0
26	37.36720158296153	39.0	36.0	41.0	31.0	41.0
27	37.329178923777114	39.0	36.0	41.0	31.0	41.0
28	37.18127000650317	39.0	36.0	41.0	31.0	41.0
29	36.9724058712557	39.0	36.0	41.0	31.0	41.0
30	36.840771236192325	39.0	35.0	41.0	30.0	41.0
31	36.58549127027244	39.0	35.0	40.0	30.0	41.0
32	36.429975442556774	38.0	35.0	40.0	30.0	41.0
33	36.25475056116078	38.0	35.0	40.0	30.0	41.0
34	36.061545904843584	38.0	35.0	40.0	30.0	41.0
35	35.86099879269286	38.0	35.0	40.0	29.0	41.0
36	35.80165953938648	38.0	35.0	40.0	28.0	41.0
37	35.65697610939203	38.0	35.0	40.0	27.0	41.0
38	35.52300449506762	38.0	35.0	40.0	26.0	41.0
39	35.39636294800981	38.0	35.0	40.0	25.0	41.0
40	35.207828946596855	38.0	34.0	40.0	25.0	41.0
41	35.07400467362816	38.0	34.0	40.0	24.0	41.0
42	34.77730411239304	38.0	34.0	40.0	23.0	41.0
43	34.40157605696362	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	7.0
13	7.0
14	17.0
15	29.0
16	54.0
17	130.0
18	347.0
19	835.0
20	1887.0
21	3975.0
22	7181.0
23	12788.0
24	20532.0
25	32606.0
26	48015.0
27	67428.0
28	92313.0
29	121773.0
30	153280.0
31	187795.0
32	230290.0
33	285048.0
34	353178.0
35	438300.0
36	581789.0
37	857684.0
38	1140445.0
39	1416230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39101141746844	21.271978763023068	14.548786093883203	22.788223725625286
2	18.17781029271871	23.981853887920472	35.819476445948105	22.020859373412716
3	19.01187468915021	24.555180246215333	33.67831913245674	22.754625932177717
4	12.949141298873515	16.32538917857029	36.84174795080715	33.88372157174905
5	12.026705125183165	39.1268604117398	35.03311629114718	13.813318171929852
6	31.395866083886453	38.30161667494498	13.729191543597267	16.573325697571295
7	25.467226541794318	34.093914256911454	21.335061345705604	19.103797855588624
8	27.400272449363385	34.34923436178811	19.025452558478577	19.22504063036993
9	24.532963416231564	14.518128520314525	22.105398953975484	38.843509109478426
10	16.071952135863267	28.256289386351334	33.178795596739924	22.492962881045475
11	32.98834202818019	22.956394391844427	23.79595931198194	20.259304267993443
12	21.395550588382598	27.737968265116653	31.023217991370622	19.84326315513013
13	31.974280013657157	21.09036237218922	26.393428839823923	20.5419287743297
14	20.314259950786003	22.90991248881519	28.787775424684202	27.988052135714604
15	22.247636220139217	30.255044252786888	25.74142682263487	21.755892704439024
16	21.272110907736728	28.66725944582802	26.86315374261084	23.197475903824415
17	21.45797244749684	27.247083690055234	27.396159445150776	23.898784417297147
18	22.81192718363771	25.545538802725947	30.252417876602937	21.390116137033406
19	22.44296262501509	24.90906378939172	31.817473792812617	20.83049979278057
20	23.345791826816424	24.58555701226749	31.151282736994524	20.917368423921562
21	22.09908904389831	25.335296563295913	32.015310947248	20.550303445557784
22	20.94072500206063	25.821853398985027	31.489325432621147	21.748096166333195
23	21.99978229158425	25.83620761850614	31.939592026321904	20.224418063587706
24	21.468610096946318	25.936323757191353	30.141664088468247	22.453402057394083
25	21.65141579020309	26.088372768344204	31.235871871824916	21.02433956962779
26	21.053064526759222	27.0486684024976	31.14639338258917	20.751873688154003
27	20.07643250237984	27.037849054066843	30.96692434335227	21.91879410020104
28	20.822092085374074	26.612623883604293	30.74989316925805	21.815390861763582
29	20.874074512109328	26.60732157696876	30.143910548600434	22.37469336232148
30	20.9629418320444	26.954482257837793	31.829961468253305	20.2526144418645
31	21.893868303587283	26.754266498556568	31.237887078708198	20.11397811914795
32	21.361853686399783	26.676268081319876	30.365913667546035	21.59596456473431
33	20.986876543305723	26.54960737327859	31.002586897950753	21.46092918546494
34	20.2303150214347	27.05671271194154	31.64566914696788	21.067303119655882
35	19.653800671922834	29.181252827690397	30.588544473881512	20.576402026505257
36	21.95832188767402	26.909156621053064	30.33852667564039	20.793994815632523
37	21.024620377144316	27.74075982219268	30.23013497426234	21.004484826400663
38	20.719233943880454	26.296798017961443	31.50039255239001	21.483575485768096
39	20.308891571793644	25.96428888221925	32.28680226145856	21.440017284528544
40	19.16579024438348	26.34688086443786	32.13615728788833	22.351171603290336
41	20.471446087682313	25.50254221651944	32.20412922497621	21.821882470822036
42	19.307895365833556	27.298438429400612	31.56966941852527	21.823996786240563
43	19.428097500994802	27.23704069181722	30.97054180488866	22.36432000229932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1987.0
1	1879.5
2	1772.0
3	4117.5
4	6463.0
5	6463.0
6	9637.5
7	12812.0
8	13556.0
9	14300.0
10	21637.5
11	28975.0
12	28975.0
13	52377.5
14	75780.0
15	129277.0
16	182774.0
17	167751.0
18	152728.0
19	152728.0
20	154053.5
21	155379.0
22	123417.5
23	91456.0
24	102227.0
25	112998.0
26	112998.0
27	125801.5
28	138605.0
29	148890.5
30	159176.0
31	173943.5
32	188711.0
33	188711.0
34	207662.0
35	226613.0
36	247250.5
37	267888.0
38	285167.5
39	302447.0
40	302447.0
41	314649.0
42	326851.0
43	343379.0
44	359907.0
45	383988.0
46	408069.0
47	408069.0
48	525361.0
49	642653.0
50	592929.5
51	543206.0
52	488447.5
53	433689.0
54	433689.0
55	383125.0
56	332561.0
57	283577.5
58	234594.0
59	209795.5
60	184997.0
61	184997.0
62	163307.5
63	141618.0
64	122626.0
65	103634.0
66	89441.0
67	75248.0
68	75248.0
69	63984.0
70	52720.0
71	44984.5
72	37249.0
73	30671.5
74	24094.0
75	24094.0
76	19235.0
77	14376.0
78	11261.0
79	8146.0
80	6479.0
81	4812.0
82	4812.0
83	3582.5
84	2353.0
85	1804.0
86	1255.0
87	919.5
88	584.0
89	584.0
90	442.5
91	301.0
92	207.0
93	113.0
94	80.5
95	48.0
96	48.0
97	34.5
98	21.0
99	13.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6053969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.73612609139984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7229444233023	28.897280893422028
2	13.50710013508252	10.734396681936056
3	5.332479712245639	6.356762586768728
4	2.6215080790115985	4.16674302308913
5	1.460459939137731	2.9016510146507515
6	0.9252424291356327	2.2059329897547952
7	0.6270274233749109	1.7440948530593705
8	0.42526109310881394	1.3518582734030686
9	0.30895608253888845	1.1049046067223187
>10	1.7693836575830717	13.208508422223808
>50	0.17280416347585376	4.786684202387805
>100	0.11356654030075859	8.304169525883296
>500	0.007676115354480504	2.119782520898323
>1k	0.0042969496555354866	3.558871359804446
>5k	7.92641198589009E-4	1.9634414934742515
>10k+	5.006154938456899E-4	6.59491755252182
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	99838	1.6491329902746446	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	73204	1.209190202328423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	71827	1.1864447934900229	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30404	0.5022159842576003	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	19409	0.32059959342375227	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	19010	0.3140088758300546	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17587	0.2905036348881205	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	15748	0.2601268688359653	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	15649	0.25849157800444633	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12589	0.20794622503022397	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	12074	0.19943940908848393	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	10494	0.17334082814100965	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	9691	0.16007680250757808	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8945	0.1477543079589605	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7928	0.1309554112351748	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	6986	0.11539537120193381	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6868	0.11344623667547686	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	6323	0.10444387805751895	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6213	0.10262688824472012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.303617841452442E-5	0.0
2	0.0	0.0	0.0	3.303617841452442E-5	0.0
3	0.0	0.0	0.0	4.955426762178663E-5	0.0
4	0.0	0.0	0.0	4.955426762178663E-5	0.0
5	0.0	0.0	0.0	1.3214471365809768E-4	0.0
6	3.303617841452442E-5	0.0	0.0	1.3214471365809768E-4	0.0
7	3.303617841452442E-5	0.0	0.0	1.3214471365809768E-4	0.0
8	3.303617841452442E-5	0.0	0.0	1.3214471365809768E-4	0.0
9	3.303617841452442E-5	0.0	0.0	1.9821707048714652E-4	0.0
10	3.303617841452442E-5	0.0	0.0	2.4777133810893314E-4	1.651808920726221E-5
11	3.303617841452442E-5	0.0	0.0	3.303617841452442E-4	1.651808920726221E-5
12	3.303617841452442E-5	0.0	0.0	4.2947031938881747E-4	1.651808920726221E-5
13	3.303617841452442E-5	0.0	0.0	5.285788546323907E-4	1.651808920726221E-5
14	9.910853524357326E-5	0.0	0.0	5.781331222541774E-4	1.651808920726221E-5
15	9.910853524357326E-5	0.0	0.0	8.259044603631105E-4	1.651808920726221E-5
16	1.1562662445083548E-4	0.0	0.0	0.0012223386013374036	3.303617841452442E-5
17	1.1562662445083548E-4	0.0	0.0	0.0016848450991407454	3.303617841452442E-5
18	1.1562662445083548E-4	0.0	0.0	0.0020647611509077763	3.303617841452442E-5
19	1.1562662445083548E-4	0.0	0.0	0.002758520897612789	3.303617841452442E-5
20	1.1562662445083548E-4	0.0	0.0	0.004757209691691517	3.303617841452442E-5
21	1.3214471365809768E-4	0.0	0.0	0.008837177725885282	3.303617841452442E-5
22	1.3214471365809768E-4	0.0	0.0	0.01501494308940135	3.303617841452442E-5
23	1.3214471365809768E-4	0.0	0.0	0.017889090611464976	3.303617841452442E-5
24	1.3214471365809768E-4	0.0	0.0	0.022382010875840297	3.303617841452442E-5
25	1.3214471365809768E-4	0.0	0.0	0.02517356795186761	3.303617841452442E-5
26	1.3214471365809768E-4	0.0	1.651808920726221E-5	0.0312522247801401	3.303617841452442E-5
27	1.3214471365809768E-4	0.0	1.651808920726221E-5	0.0722996764601867	4.955426762178663E-5
28	1.3214471365809768E-4	0.0	1.651808920726221E-5	0.1739685155308856	4.955426762178663E-5
29	1.3214471365809768E-4	0.0	1.651808920726221E-5	0.324993405152884	4.955426762178663E-5
30	1.486628028653599E-4	0.0	1.651808920726221E-5	0.5281328662237946	4.955426762178663E-5
31	1.486628028653599E-4	0.0	1.651808920726221E-5	0.7982531790301536	4.955426762178663E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22560	0.0	23.617022	1
GTATTGG	1805	0.0	20.396122	1
ATTGGAC	1950	0.0	18.40513	3
TTGGACC	2620	0.0	17.864504	4
GGACCCT	2700	0.0	16.787037	6
TGGACCC	2915	0.0	16.056604	5
GACCCTC	2885	0.0	15.646447	7
GTATCAA	37260	0.0	14.289587	2
TATTGGA	2670	0.0	14.134831	2
ACGTTCG	485	0.0	14.113402	22
GATACCT	2370	0.0	13.972573	36
CCCTCGC	3095	0.0	13.927301	9
ACCCTCG	3235	0.0	13.839258	8
TAATACT	1215	0.0	13.551441	4
CTAATAC	985	0.0	13.522841	3
CGTTCGC	575	0.0	13.513043	23
CCTCGCA	3205	0.0	13.333854	10
GTATTAG	960	0.0	13.296876	1
ACCGTTA	335	0.0	13.253732	8
CTAGTAC	830	0.0	13.150602	3
>>END_MODULE