FastQCFastQC Report
Wed 25 May 2016
SRR2088508_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088508_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5084344
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT821941.6166097337237606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT606411.1927005725812416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT578771.1383376105157323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239050.4701688162720697No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG141620.2785413418132211No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA136060.26760581109382053No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA112900.22205421191013036No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG112450.22116914197780482No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG103360.20329072934482795No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA98580.1938893198414584No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91740.1804362568701095No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC73410.14438440829338062No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT69970.13761854036626947No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66240.13028229403832628No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT57050.11220719919816599No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA52410.10308114478485328No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51380.1010553180508636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185700.023.650511
GTATTGG14550.019.7079051
ATTGGAC16050.018.788163
TTGGACC22350.016.8031314
GGACCCT21650.016.4919176
CTAATAC10550.015.7819913
TGGACCC22700.015.72907455
GACCCTC22150.015.618517
GTATCAA301750.014.5057162
TAATACT13150.014.349814
TATTGGA19900.014.3165832
TCTAACG3050.013.950822
ACCCTCG25500.013.7117648
TGTTACG2304.0017767E-1113.67391316
CCCTCGC24600.013.5365859
TCTACAC7450.013.4093953
GATACCT18050.013.22160636
CCGTTTA3650.013.17808227
CCTCGCA25250.013.11485210
CGTTTAC4100.013.08536528
Produced by FastQC (version 0.11.5)