##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5084344
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673353533907225	31.0	31.0	34.0	30.0	34.0
2	31.834090297588048	31.0	31.0	34.0	30.0	34.0
3	31.952330723491567	31.0	31.0	34.0	30.0	34.0
4	35.640607716551045	37.0	35.0	37.0	33.0	37.0
5	35.5537550173631	37.0	35.0	37.0	33.0	37.0
6	35.60058446084687	37.0	35.0	37.0	33.0	37.0
7	35.453201632304975	37.0	35.0	37.0	33.0	37.0
8	35.493739408663146	37.0	35.0	37.0	33.0	37.0
9	37.082707621671545	39.0	37.0	39.0	33.0	39.0
10	36.93783996519512	39.0	37.0	39.0	33.0	39.0
11	37.00097220015011	39.0	37.0	39.0	33.0	39.0
12	36.850525062820296	39.0	37.0	39.0	32.0	39.0
13	36.904664790580654	39.0	37.0	39.0	33.0	39.0
14	37.9179725054009	40.0	37.0	41.0	33.0	41.0
15	37.92638834036406	40.0	37.0	41.0	33.0	41.0
16	37.81247511970079	40.0	37.0	41.0	32.0	41.0
17	37.85733970793479	40.0	37.0	41.0	32.0	41.0
18	37.82884635658012	40.0	37.0	41.0	32.0	41.0
19	37.896568367521944	40.0	37.0	41.0	32.0	41.0
20	37.79461932552164	40.0	37.0	41.0	32.0	41.0
21	37.66408016452073	40.0	37.0	41.0	32.0	41.0
22	37.547562478069935	39.0	36.0	41.0	32.0	41.0
23	37.380102329818754	39.0	36.0	41.0	31.0	41.0
24	37.52616129042409	39.0	36.0	41.0	32.0	41.0
25	37.4819650283301	39.0	36.0	41.0	32.0	41.0
26	37.27335601210304	39.0	36.0	41.0	31.0	41.0
27	37.23369622511773	39.0	36.0	41.0	31.0	41.0
28	37.08474446260914	39.0	36.0	41.0	31.0	41.0
29	36.87127031530518	39.0	35.0	41.0	30.0	41.0
30	36.744717705961676	39.0	35.0	40.0	30.0	41.0
31	36.49486954462562	39.0	35.0	40.0	30.0	41.0
32	36.34067738138883	38.0	35.0	40.0	30.0	41.0
33	36.173978983326066	38.0	35.0	40.0	30.0	41.0
34	35.98466645844577	38.0	35.0	40.0	29.0	41.0
35	35.7939063525206	38.0	35.0	40.0	28.0	41.0
36	35.729785199427894	38.0	35.0	40.0	27.0	41.0
37	35.58985387298735	38.0	35.0	40.0	27.0	41.0
38	35.4508174505895	38.0	35.0	40.0	26.0	41.0
39	35.32408743389511	38.0	34.0	40.0	25.0	41.0
40	35.14415370006436	38.0	34.0	40.0	25.0	41.0
41	35.01037341297127	38.0	34.0	40.0	24.0	41.0
42	34.730005876864354	38.0	34.0	40.0	23.0	41.0
43	34.340922447418976	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	4.0
13	6.0
14	3.0
15	22.0
16	66.0
17	143.0
18	357.0
19	763.0
20	1703.0
21	3414.0
22	6500.0
23	11012.0
24	18193.0
25	28163.0
26	41225.0
27	57996.0
28	79362.0
29	104652.0
30	131571.0
31	161378.0
32	198861.0
33	246087.0
34	304107.0
35	379615.0
36	498642.0
37	724016.0
38	967978.0
39	1118499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23945980051704	21.334945078460464	14.598913842179051	22.82668127884345
2	18.43221072374332	24.0002053362243	35.37732694719319	22.190256992839196
3	19.049320817002155	24.436308794212195	33.78248993380464	22.731880454981017
4	13.236594534122789	16.626609057136967	36.675193495955426	33.46160291278481
5	12.066591088250519	39.151815848809605	35.049300362052605	13.732292700887271
6	31.521057583829894	38.432608021801826	13.847548474296783	16.198785920071497
7	25.49801901680925	34.148476184931624	21.25945844734345	19.094046350915676
8	27.398696862368084	34.40671992296351	19.01995616346966	19.17462705119874
9	24.667744747404974	14.452700289358864	21.887150043348758	38.992404919887406
10	16.258537974613834	28.39058490141501	33.00850611209627	22.342371011874885
11	33.36339948673811	22.945575673085848	23.411220798592698	20.27980404158334
12	21.441350152546722	27.883636512399633	30.701286144289213	19.973727190764436
13	31.85992135858628	21.168787949831877	26.06013676493959	20.911153926642257
14	20.48319311203176	22.93344038090263	28.57151286380308	28.011853643262537
15	22.545681409440434	30.405967810203244	25.269572633165655	21.77877814719067
16	21.40671441586171	28.62027038296386	26.6658589584025	23.307156242771928
17	21.60178382894627	27.43437501475117	27.272525226459894	23.69131592984267
18	22.72753377820226	25.691632981560648	30.00756833133242	21.57326490890467
19	22.57115175527069	25.098557454019634	31.49696401344992	20.83332677725976
20	23.29053659626493	24.920678065842907	30.76172658655669	21.027058751335474
21	22.24692113672875	25.52260429270718	31.499402872818994	20.731071697745076
22	21.08793189445875	26.13434496170991	31.006890957810878	21.77083218602046
23	22.10062891102569	26.039976838703282	31.448127821406263	20.411266428864767
24	21.553104982668366	26.2022003231882	29.887002138328956	22.357692555814477
25	21.727542432219376	26.152321715446476	30.982600705223724	21.137535147110423
26	21.12164322477	27.245796114503662	30.8313127514582	20.801247909268135
27	20.222294164202896	27.15363083221749	30.57002043921497	22.054054564364645
28	20.905489479075374	26.875954892115878	30.444124158396836	21.774431470411916
29	21.03205447939793	26.840276739732793	29.780066022283307	22.347602758585968
30	21.03913503885654	27.174321800413193	31.365639303713515	20.420903857016757
31	21.802301339169812	26.95645691951607	30.956874672524126	20.284367068789997
32	21.175337467331083	26.8780200552913	30.172427357393598	21.774215119984014
33	20.861275318900528	26.758181586454416	30.83150943366538	21.549033660979667
34	20.286904269262664	27.161989826022786	31.41602928519392	21.13507661952063
35	19.772245937725692	29.076592771850212	30.416057607431757	20.73510368299234
36	21.839159584795993	27.178137435232546	30.048320884660836	20.934382095310625
37	20.86438289777403	27.846561916345554	30.133110584177626	21.155944601702796
38	20.66325567270822	26.462351878629768	31.281656001246176	21.59273644741583
39	20.19330320686405	26.28533789216465	32.00420742577607	21.517151475195227
40	19.246258711054956	26.483377206577686	32.020886076945224	22.249478005422134
41	20.20119016337211	25.730733404348722	32.10205682384984	21.966019608429328
42	19.37359077198553	27.216352788088294	31.463056000931488	21.94700043899469
43	19.262603002471902	27.037037619799133	31.07588707609084	22.624472301638125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1866.0
1	1734.0
2	1602.0
3	3398.0
4	5194.0
5	5194.0
6	7868.0
7	10542.0
8	11052.0
9	11562.0
10	17364.5
11	23167.0
12	23167.0
13	42106.0
14	61045.0
15	105679.0
16	150313.0
17	138257.5
18	126202.0
19	126202.0
20	127768.5
21	129335.0
22	102553.0
23	75771.0
24	84335.0
25	92899.0
26	92899.0
27	103712.5
28	114526.0
29	123047.0
30	131568.0
31	145832.0
32	160096.0
33	160096.0
34	177364.0
35	194632.0
36	211748.0
37	228864.0
38	244403.0
39	259942.0
40	259942.0
41	271465.0
42	282988.0
43	298260.5
44	313533.0
45	330260.0
46	346987.0
47	346987.0
48	430494.5
49	514002.0
50	478241.5
51	442481.0
52	399172.5
53	355864.0
54	355864.0
55	316685.5
56	277507.0
57	241168.5
58	204830.0
59	183666.5
60	162503.0
61	162503.0
62	143230.0
63	123957.0
64	106462.0
65	88967.0
66	76252.0
67	63537.0
68	63537.0
69	54554.5
70	45572.0
71	39178.0
72	32784.0
73	26941.5
74	21099.0
75	21099.0
76	16971.0
77	12843.0
78	10017.5
79	7192.0
80	5667.0
81	4142.0
82	4142.0
83	3150.5
84	2159.0
85	1636.5
86	1114.0
87	862.5
88	611.0
89	611.0
90	469.0
91	327.0
92	230.5
93	134.0
94	96.0
95	58.0
96	58.0
97	39.0
98	20.0
99	13.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5084344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.258293305925946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74699521997647	31.16422154436484
2	13.300109792653195	11.240798812379134
3	5.207797829237164	6.6021794443760555
4	2.4069309963107086	4.068511840368899
5	1.346007498472873	2.8439989831221166
6	0.8396045873282201	2.1288154147390106
7	0.5621786442931537	1.662969702860753
8	0.3734197129321235	1.2624063804240275
9	0.2903727333813178	1.1043590521744047
>10	1.666387906939589	13.286793879907496
>50	0.15586112175233907	4.5878379155865066
>100	0.09243878305965283	7.338651417498511
>500	0.0065773319932177126	1.8641163298862367
>1k	0.004478182459027748	4.015581618293332
>5k	4.1982960553378466E-4	1.205638428509671
>10k+	4.1982960553378466E-4	5.623119235508817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	82194	1.6166097337237606	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	60641	1.1927005725812416	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	57877	1.1383376105157323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23905	0.4701688162720697	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14162	0.2785413418132211	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13606	0.26760581109382053	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11290	0.22205421191013036	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11245	0.22116914197780482	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10336	0.20329072934482795	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9858	0.1938893198414584	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9174	0.1804362568701095	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7341	0.14438440829338062	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6997	0.13761854036626947	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6624	0.13028229403832628	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5705	0.11220719919816599	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5241	0.10308114478485328	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5138	0.1010553180508636	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.933644143669272E-5	0.0	0.0	0.0	0.0
6	9.834110359173179E-5	0.0	0.0	0.0	0.0
7	9.834110359173179E-5	0.0	0.0	0.0	0.0
8	9.834110359173179E-5	0.0	0.0	0.0	0.0
9	9.834110359173179E-5	0.0	0.0	9.834110359173179E-5	0.0
10	9.834110359173179E-5	0.0	0.0	9.834110359173179E-5	0.0
11	9.834110359173179E-5	0.0	0.0	1.3767754502842452E-4	0.0
12	9.834110359173179E-5	0.0	0.0	2.1635042790180996E-4	0.0
13	9.834110359173179E-5	0.0	0.0	2.1635042790180996E-4	0.0
14	1.1800932431007816E-4	0.0	0.0	3.343597522118881E-4	0.0
15	1.1800932431007816E-4	0.0	0.0	4.720372972403126E-4	0.0
16	1.1800932431007816E-4	0.0	0.0	8.850699323255861E-4	0.0
17	1.1800932431007816E-4	0.0	0.0	0.0011407568016640888	0.0
18	1.1800932431007816E-4	0.0	0.0	0.0013571072295658988	0.0
19	1.1800932431007816E-4	0.0	0.0	0.0019471538511162895	0.0
20	1.1800932431007816E-4	0.0	0.0	0.003009237769906993	0.0
21	1.1800932431007816E-4	0.0	0.0	0.005113737386770054	0.0
22	1.1800932431007816E-4	0.0	0.0	0.008142643377395392	0.0
23	1.1800932431007816E-4	0.0	0.0	0.010168470111385067	0.0
24	1.1800932431007816E-4	0.0	0.0	0.013118703219137022	0.0
25	1.1800932431007816E-4	0.0	0.0	0.0154002168224652	0.0
26	1.1800932431007816E-4	0.0	0.0	0.020120589794868325	0.0
27	1.1800932431007816E-4	0.0	0.0	0.059162007920785846	0.0
28	1.1800932431007816E-4	0.0	0.0	0.15445453730117395	0.0
29	1.1800932431007816E-4	0.0	0.0	0.30530979021089055	0.0
30	1.3767754502842452E-4	0.0	0.0	0.5099576267852844	0.0
31	1.3767754502842452E-4	0.0	0.0	0.7761866624288207	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18570	0.0	23.65051	1
GTATTGG	1455	0.0	19.707905	1
ATTGGAC	1605	0.0	18.78816	3
TTGGACC	2235	0.0	16.803131	4
GGACCCT	2165	0.0	16.491917	6
CTAATAC	1055	0.0	15.781991	3
TGGACCC	2270	0.0	15.7290745	5
GACCCTC	2215	0.0	15.61851	7
GTATCAA	30175	0.0	14.505716	2
TAATACT	1315	0.0	14.34981	4
TATTGGA	1990	0.0	14.316583	2
TCTAACG	305	0.0	13.95082	2
ACCCTCG	2550	0.0	13.711764	8
TGTTACG	230	4.0017767E-11	13.673913	16
CCCTCGC	2460	0.0	13.536585	9
TCTACAC	745	0.0	13.409395	3
GATACCT	1805	0.0	13.221606	36
CCGTTTA	365	0.0	13.178082	27
CCTCGCA	2525	0.0	13.114852	10
CGTTTAC	410	0.0	13.085365	28
>>END_MODULE