FastQCFastQC Report
Wed 25 May 2016
SRR2088507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629474
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67481.0720061511674828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50480.8019393970203693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48880.7765213495712293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.3221737514178505No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG12010.19079421866510768No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10790.1714129574851384No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG10460.16617048519875324No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA9750.15489122664319735No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG9470.15044306833959784No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8900.14138788893584167No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7240.11501666470735886No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC6410.10183105259311742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.005494478629.5999984
GTACTAG509.08256E-625.8999981
GGTATCA12650.023.8379461
TATACCG400.001930221723.1250025
TAGAACC603.722839E-521.5833344
TCTAAAC806.949831E-720.81253
CGTTCGC555.139543E-420.18181823
TACACTA701.2180857E-418.55
GTTTAGG1002.8698014E-718.4999981
ATGCGAC500.007031433718.49999821
GGACCCT1900.017.5263166
CTAATAT752.0653944E-417.2666663
GACCCTC1851.8189894E-1217.07
GTATTGG1757.2759576E-1216.9142861
CTAAACA904.4408174E-516.4444454
CTTATCC1059.329597E-615.8571423
AGGCTAT700.00259074715.8571426
TTGGACC1802.0008883E-1015.4166664
TGGACCC2300.015.2826075
TGCCGAA2300.015.28260730
Produced by FastQC (version 0.11.5)