FastQCFastQC Report
Wed 25 May 2016
SRR2088506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815206
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148551.8222387960834439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103121.2649563423232901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98231.2049715041351512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43560.5343434665593727No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24130.29599880275660384No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20020.24558209826718644No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA19760.2423927203676126No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18450.2263231624889905No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA16760.2055922061417605No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15140.18571992845980034No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14790.18142653513345092No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.17161306467322368No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12460.1528448024180391No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT10990.1348125504473716No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9210.112977578673366No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT9100.11162822648508475No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8800.10794817506249953No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.10782550668174669No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG8160.10009739869431776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC451.3227512E-424.6666663
AGCGCTA451.3227512E-424.66666618
GGTATCA31100.024.2700981
CTCCGTT400.001930708123.12512
GCGCTAT502.701169E-422.19999919
TGGACCC3100.020.2903215
GGACCCT3150.019.3809536
GTATTGG2400.018.51
TCTAGTA701.21864534E-418.52
ACTAACT609.233678E-418.58
GACCGTG500.007033171618.4999987
ATTTGCG500.007033171618.49999824
TTGGACC4150.017.8313264
GACCCTC3450.017.6956527
TTTTTAC5100.017.4117641
ACCCCGC650.001579401317.0769236
CTAATAC650.001579401317.0769233
ATTGGAC2700.016.4444453
TAGTACC904.4434062E-516.4444454
ATACCGT803.3810563E-416.18756
Produced by FastQC (version 0.11.5)