##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815206
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456166907505587	31.0	31.0	34.0	30.0	34.0
2	31.614422612198634	31.0	31.0	34.0	30.0	34.0
3	31.72611462624171	31.0	31.0	34.0	30.0	34.0
4	35.44517827395775	37.0	35.0	37.0	33.0	37.0
5	35.33967733309127	37.0	35.0	37.0	33.0	37.0
6	35.41256443156699	37.0	35.0	37.0	33.0	37.0
7	35.24314958427686	37.0	35.0	37.0	32.0	37.0
8	35.31207326737046	37.0	35.0	37.0	32.0	37.0
9	36.840044111549716	39.0	37.0	39.0	32.0	39.0
10	36.671088044003604	39.0	35.0	39.0	32.0	39.0
11	36.76469015193705	39.0	37.0	39.0	32.0	39.0
12	36.577542363525296	38.0	35.0	39.0	32.0	39.0
13	36.65391569738201	39.0	35.0	39.0	32.0	39.0
14	37.57226271641769	39.0	36.0	41.0	32.0	41.0
15	37.596092276062734	39.0	36.0	41.0	32.0	41.0
16	37.478471699177874	39.0	36.0	41.0	32.0	41.0
17	37.53106454074185	39.0	36.0	41.0	32.0	41.0
18	37.513231011548	39.0	36.0	41.0	32.0	41.0
19	37.59459449513375	39.0	37.0	41.0	32.0	41.0
20	37.493963488983155	39.0	36.0	41.0	32.0	41.0
21	37.342030112634106	39.0	36.0	41.0	31.0	41.0
22	37.223511603202134	39.0	36.0	41.0	31.0	41.0
23	37.03266781647829	39.0	36.0	40.0	31.0	41.0
24	37.1679268798316	39.0	36.0	41.0	31.0	41.0
25	37.10558680873301	39.0	36.0	41.0	31.0	41.0
26	36.86406503386874	39.0	36.0	41.0	30.0	41.0
27	36.80653724334708	39.0	35.0	40.0	30.0	41.0
28	36.65354278550452	39.0	35.0	40.0	30.0	41.0
29	36.41700748031786	39.0	35.0	40.0	30.0	41.0
30	36.2643061999053	38.0	35.0	40.0	30.0	41.0
31	35.977794569716124	38.0	35.0	40.0	29.0	41.0
32	35.80264129557437	38.0	35.0	40.0	29.0	41.0
33	35.61603324803792	38.0	34.0	40.0	27.0	41.0
34	35.38381096311853	38.0	34.0	40.0	27.0	41.0
35	35.178884846284255	38.0	34.0	40.0	26.0	41.0
36	35.1009744776167	38.0	34.0	40.0	25.0	41.0
37	34.93936133934245	38.0	34.0	40.0	25.0	41.0
38	34.78521011866939	38.0	33.0	40.0	24.0	41.0
39	34.631962963962486	38.0	33.0	40.0	24.0	41.0
40	34.44159513055596	38.0	33.0	40.0	23.0	41.0
41	34.28654107060056	38.0	33.0	40.0	22.0	41.0
42	33.96214944443491	38.0	33.0	40.0	20.0	41.0
43	33.55169490901686	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	9.0
16	14.0
17	33.0
18	95.0
19	184.0
20	400.0
21	797.0
22	1417.0
23	2384.0
24	3928.0
25	5986.0
26	8344.0
27	11464.0
28	15348.0
29	19808.0
30	24379.0
31	29241.0
32	35541.0
33	43391.0
34	52564.0
35	65125.0
36	84824.0
37	119277.0
38	155529.0
39	135120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69941339980324	22.624956146053883	15.804103502672945	21.871526951469935
2	18.992868060343028	24.691673024977735	34.97888877167243	21.336570143006796
3	19.73746513151277	24.85948336984762	33.647446166981105	21.7556053316585
4	14.005785040836304	17.19577628231392	35.93214475850276	32.86629391834702
5	11.895152881602932	39.980937333631005	34.47594841058579	13.647961374180268
6	31.510808311028132	38.721623736822345	13.467884191235099	16.29968376091442
7	24.53600684980238	34.84910562483593	21.29903361849643	19.315853906865257
8	27.307821581293563	34.91767724967677	18.834012507268103	18.940488661761567
9	24.428672016643645	14.841034045382395	22.24443882895857	38.48585510901539
10	16.193330274801706	29.104913359322676	32.799684987598226	21.902071378277395
11	32.93719624242216	23.42733002455821	23.373233268646207	20.26224046437342
12	20.96218624494913	28.57437261256664	30.905317183632114	19.558123958852118
13	31.82717006498971	21.851532005407222	26.249438792158053	20.071859137445013
14	19.922081044545795	23.73571833377085	29.063819451770474	27.27838116991288
15	21.92709572795097	31.187209122602138	25.763303999234548	21.122391150212337
16	20.64275778146873	29.22230699970314	27.03868715392183	23.096248064906295
17	21.033088569024272	27.952321253768986	27.847930461748323	23.166659715458422
18	22.11698638135637	26.219630375635116	30.652497650900507	21.010885592108007
19	22.058718900498768	25.651307767607207	32.224247613486654	20.06572571840737
20	22.751304578229306	25.40658434800529	31.564905066940135	20.277206006825267
21	21.672068164365818	25.982267058878367	32.24706393230668	20.098600844449134
22	20.315110536477896	26.607507795575597	31.741056861701217	21.336324806245294
23	21.41740860592292	26.643694967897684	32.17812430232358	19.760772123855812
24	20.918025627878105	26.754219178955996	30.665623167641066	21.662132025524837
25	21.040694008630947	26.93147498914385	31.604281617161796	20.423549385063406
26	20.536158958594513	27.956982652237595	31.47130909242572	20.035549296742172
27	19.452506483023924	27.80732722771913	31.28779719481947	21.45236909443748
28	20.193178166009574	27.561990466213448	31.159118063409743	21.08571330436724
29	20.375463379808295	27.230417833038523	30.393176693989005	22.000942093164184
30	20.456301842724418	27.630930096196543	32.13997443590945	19.772793625169587
31	21.382693454169864	27.473791900452156	31.6107830413417	19.53273160403628
32	20.519721395573633	27.398228177908408	30.923962777506542	21.158087649011414
33	20.29462491689217	27.055860727227227	31.57778524691918	21.071729108961414
34	19.60363392811142	27.423620532724247	32.2673042151309	20.705441324033437
35	19.143504831907517	29.06013940034789	31.32422970390306	20.472126063841532
36	21.318292554274628	27.106277431716645	30.997809142719756	20.577620871288975
37	20.242613523452967	27.829407536254642	31.202665338576995	20.725313601715396
38	19.997276761947287	26.344752124003012	32.36470290944865	21.293268204601045
39	19.359769187174773	26.19620071491132	33.18400502449688	21.260025073417026
40	18.464780681202054	26.47367659217425	33.01361864363118	22.04792408299252
41	19.323950019994946	25.739997006891507	33.208048027124434	21.728004945989113
42	18.682639725419097	27.37038245547751	32.2324663949971	21.714511424106302
43	18.64044180244012	27.26721834726437	31.897091042018825	22.19524880827668
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	524.0
1	402.0
2	280.0
3	517.5
4	755.0
5	755.0
6	1210.5
7	1666.0
8	1841.0
9	2016.0
10	3112.5
11	4209.0
12	4209.0
13	7723.5
14	11238.0
15	19564.5
16	27891.0
17	25557.5
18	23224.0
19	23224.0
20	23418.0
21	23612.0
22	19103.5
23	14595.0
24	15911.0
25	17227.0
26	17227.0
27	18921.0
28	20615.0
29	21717.5
30	22820.0
31	24657.0
32	26494.0
33	26494.0
34	29203.5
35	31913.0
36	34512.5
37	37112.0
38	38986.5
39	40861.0
40	40861.0
41	42109.5
42	43358.0
43	45594.0
44	47830.0
45	51166.0
46	54502.0
47	54502.0
48	69327.0
49	84152.0
50	77264.5
51	70377.0
52	62523.5
53	54670.0
54	54670.0
55	48394.0
56	42118.0
57	36515.0
58	30912.0
59	27446.5
60	23981.0
61	23981.0
62	20747.5
63	17514.0
64	15124.5
65	12735.0
66	10771.5
67	8808.0
68	8808.0
69	7551.0
70	6294.0
71	5318.0
72	4342.0
73	3600.0
74	2858.0
75	2858.0
76	2266.0
77	1674.0
78	1313.0
79	952.0
80	731.0
81	510.0
82	510.0
83	391.5
84	273.0
85	202.5
86	132.0
87	107.0
88	82.0
89	82.0
90	67.0
91	52.0
92	36.0
93	20.0
94	13.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	815206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.750760717844976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4087878726295	48.74671710915562
2	8.933959347601668	10.318858970925968
3	2.592322626881209	4.491258111854162
4	1.1068337280845129	2.55681959140196
5	0.6785040031364638	1.9592061165616932
6	0.4511183853369056	1.5631457956207333
7	0.29909610703304856	1.2091119396233176
8	0.21791278814090237	1.0067703428226962
9	0.18190589291111575	0.9454683325208201
>10	1.0081729980649088	10.882141391040173
>50	0.07581355737354468	3.049421455912438
>100	0.037480345488804495	4.279105421409747
>500	0.005110956200462108	2.105486141148883
>1k	0.002342521591878466	2.5832789950252506
>5k	2.1295650835258782E-4	1.2080718670856003
>10k+	4.2591301670517564E-4	3.0951384178910013
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14855	1.8222387960834439	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10312	1.2649563423232901	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9823	1.2049715041351512	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4356	0.5343434665593727	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2413	0.29599880275660384	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2002	0.24558209826718644	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1976	0.2423927203676126	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1845	0.2263231624889905	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1676	0.2055922061417605	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1514	0.18571992845980034	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1479	0.18142653513345092	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1399	0.17161306467322368	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1246	0.1528448024180391	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1099	0.1348125504473716	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	921	0.112977578673366	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	910	0.11162822648508475	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	880	0.10794817506249953	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	879	0.10782550668174669	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	816	0.10009739869431776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.4533676150568075E-4	0.0
12	0.0	0.0	0.0	6.133419037642019E-4	0.0
13	0.0	0.0	0.0	7.360102845170424E-4	0.0
14	0.0	0.0	0.0	0.0011040154267755635	0.0
15	0.0	0.0	0.0	0.0011040154267755635	0.0
16	0.0	0.0	0.0	0.0014720205690340847	0.0
17	0.0	0.0	0.0	0.0024533676150568077	0.0
18	0.0	0.0	0.0	0.0025760359958096482	0.0
19	1.2266838075284037E-4	0.0	0.0	0.0036800514225852118	0.0
20	1.2266838075284037E-4	0.0	0.0	0.007237434464417583	0.0
21	2.4533676150568075E-4	0.0	0.0	0.011408159410014156	0.0
22	2.4533676150568075E-4	0.0	0.0	0.021098961489488546	0.0
23	2.4533676150568075E-4	0.0	0.0	0.02526968643508512	0.0
24	2.4533676150568075E-4	0.0	0.0	0.03164844223423282	0.0
25	2.4533676150568075E-4	0.0	0.0	0.03508315689531235	0.0
26	2.4533676150568075E-4	0.0	0.0	0.0434246067865055	0.0
27	2.4533676150568075E-4	0.0	0.0	0.1008334089788348	0.0
28	2.4533676150568075E-4	0.0	0.0	0.24018468951406147	0.0
29	2.4533676150568075E-4	0.0	0.0	0.4445502118482936	0.0
30	2.4533676150568075E-4	0.0	0.0	0.7324529014752099	0.0
31	2.4533676150568075E-4	0.0	0.0	1.1432693086164725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	45	1.3227512E-4	24.666666	3
AGCGCTA	45	1.3227512E-4	24.666666	18
GGTATCA	3110	0.0	24.270098	1
CTCCGTT	40	0.0019307081	23.125	12
GCGCTAT	50	2.701169E-4	22.199999	19
TGGACCC	310	0.0	20.290321	5
GGACCCT	315	0.0	19.380953	6
GTATTGG	240	0.0	18.5	1
TCTAGTA	70	1.21864534E-4	18.5	2
ACTAACT	60	9.233678E-4	18.5	8
GACCGTG	50	0.0070331716	18.499998	7
ATTTGCG	50	0.0070331716	18.499998	24
TTGGACC	415	0.0	17.831326	4
GACCCTC	345	0.0	17.695652	7
TTTTTAC	510	0.0	17.411764	1
ACCCCGC	65	0.0015794013	17.076923	6
CTAATAC	65	0.0015794013	17.076923	3
ATTGGAC	270	0.0	16.444445	3
TAGTACC	90	4.4434062E-5	16.444445	4
ATACCGT	80	3.3810563E-4	16.1875	6
>>END_MODULE