FastQCFastQC Report
Wed 25 May 2016
SRR2088505_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088505_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936212
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202422.162117127317317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175321.872652775226124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT109551.170140950981188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.669826919543864No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG34810.3718174943282077No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA32560.34778447616565483No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG22160.23669852554763235No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.22676487804044382No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT20880.22302640854849118No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG20470.21864705857220376No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19590.20924747813529415No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16160.1726104771141579No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC15590.16652211251297783No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA14690.15690890524795664No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.15584077110739877No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT14530.155199890623064No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG13250.1415277736239228No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.13853699803036065No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT11390.1216604786095457No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11080.11834926277381617No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG10790.11525167376619826No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10430.11140639086018979No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10100.10788154819634868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9640.10296813114978232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA250.005495829529.59999814
GGTATCA46750.026.3946511
GCCGATA502.7015837E-422.1999992
CGTTTAT1005.347829E-1022.1999991
CCGTATA656.90038E-519.9230792
TATACCG656.90038E-519.9230795
CGAGTTC759.2627615E-619.73333414
CGTATAC759.2627615E-619.7333343
CGTTCGC1306.9667294E-1018.50000223
AGCCGTC902.1513479E-618.512
TATTACC902.1513479E-618.52
CTCGTTT500.00703393218.4999989
TTCGTTA1002.873512E-718.49999830
CGTGTCT1002.873512E-718.49999835
GTATCAA69750.017.7440872
GTATACT752.0667506E-417.2666664
TGTATAC650.001579637517.0769253
CCGAGTT650.001579637517.07692513
GATAGAC650.001579637517.0769253
ACGTTCG1107.803792E-716.81818222
Produced by FastQC (version 0.11.5)